Microorganisms,
Год журнала:
2022,
Номер
10(5), С. 944 - 944
Опубликована: Апрель 30, 2022
The
dynamics
of
microbial
processes
are
difficult
to
study
in
natural
soil,
owing
the
small
spatial
scales
on
which
microorganisms
operate
and
opacity
chemical
complexity
soil
habitat.
To
circumvent
these
challenges,
we
have
created
a
3D-bioprinted
habitat
that
mimics
aspects
aggregates
while
providing
chemically
defined
translucent
alternative
culturing
method
for
microorganisms.
Our
Synthetic
Soil
Aggregates
(SSAs)
retain
porosity,
permeability,
patchy
resource
distribution
aggregates-parameters
expected
influence
emergent
community
interactions.
We
demonstrate
printability
viability
several
different
within
SSAs
show
how
can
be
integrated
into
multi-omics
workflow
single
SSA
resolution
genomics,
metabolomics,
proteomics,
lipidomics,
biogeochemical
assays.
impact
structured
model
co-culture
find
it
is
significantly
from
organization
same
liquid
culture,
indicating
potential
reproduce
naturally
occurring
phenotypes.
as
tool
help
researchers
quantify
scale
situ
achieve
high-resolution
data
interplay
between
environmental
properties
ecology.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(26), С. 9474 - 9494
Опубликована: Июнь 19, 2023
The
global
spread
of
antimicrobial
resistance
(AMR)
is
concerning
for
the
health
humans,
animals,
and
environment
in
a
One
Health
perspective.
Assessments
AMR
associated
environmental
hazards
mostly
focus
on
parent
compounds,
while
largely
overlooking
their
transformation
products
(TPs).
This
review
lists
TPs
identified
surface
water
environments
examines
potential
promotion,
ecological
risk,
as
well
human
using
silico
models.
Our
also
summarizes
key
compartments
TPs,
related
pathways
reaching
waters
methodologies
studying
fate
TPs.
56
covered
by
were
prioritized
via
scoring
ranking
various
risk
hazard
parameters.
Most
data
occurrences
to
date
have
been
reported
Europe,
little
known
about
antibiotic
Africa,
Central
South
America,
Asia,
Oceania.
Occurrence
antiviral
other
antibacterial
are
even
scarcer.
We
propose
evaluation
structural
similarity
between
compounds
TP
assessment.
predicted
13
especially
tetracyclines
macrolides.
estimated
ecotoxicological
effect
concentrations
from
experimental
chemical
bacteria,
algae
fleas,
scaled
potency
differences
quantitative
structure–activity
relationships
(QSARs)
baseline
toxicity
scaling
factor
similarity.
Inclusion
mixtures
with
increased
quotient
over
threshold
one
7
24
antimicrobials
included
this
analysis,
only
had
above
one.
Thirteen
which
6
macrolide
posed
at
least
three
tested
species.
There
12/21
that
likely
exhibit
similar
or
higher
level
mutagenicity/carcinogenicity,
respectively,
than
compound,
tetracycline
often
showing
mutagenicity.
carcinogenicity
belonged
sulfonamides.
be
mobile
but
not
bioaccumulative,
14
persistent.
six
highest-priority
originated
family
antivirals.
review,
particular
our
concern,
can
support
authorities
planning
intervention
strategies
source
mitigation
toward
sustainable
future.
Biosensors,
Год журнала:
2022,
Номер
12(2), С. 64 - 64
Опубликована: Янв. 25, 2022
In
recent
years,
small-molecule
biosensors
have
become
increasingly
important
in
synthetic
biology
and
biochemistry,
with
numerous
new
applications
continuing
to
be
developed
throughout
the
field.
For
many
biosensors,
however,
their
utility
is
hindered
by
poor
functionality.
Here,
we
review
known
types
of
mechanisms
within
bacterial
cells,
approaches
for
optimizing
different
biosensor
functional
parameters.
Discussed
improving
functionality
include
methods
directly
engineering
genes,
considerations
choosing
genetic
reporters,
tuning
gene
expression,
strategies
incorporating
additional
modules.
Water Research,
Год журнала:
2022,
Номер
217, С. 118384 - 118384
Опубликована: Апрель 4, 2022
Due
to
the
sheer
number
of
contaminated
sites,
bioavailability-based
measurement
and
modeling
toxicity
is
used
triage
response;
despite
advances,
both
remain
relatively
cumbersome.
Cadmium
(Cd)
lead
(Pb)
are
two
most
toxic
globally
prevalent
pollutants,
disproportionately
impacting
disadvantaged
communities.
Here
we
demonstrate
use
high
throughput
lights-on
bioreporter
technology
measure
speciation
toxicity.
The
organism's
response
fit-for-purpose
parameterize
Biotic
Ligand
Model
in
risk
assessment
aquatic
ecotoxicity
setting
environmental
Water
Quality
Criteria.
Toxicity
endpoints
for
analogous
Cd
Pb
models
reported
literature
average
71st
44th
rank-percentile
sensitivity
Genus
Mean
Acute
Values
acute
(i.e.,
insensitive)
comparison
bioreporter,
unique
dual-mode
ability
which
can
predict
from
below
5th
percentile
up
50th
rank-percentile.
These
results
extensible
other
reporters,
paving
way
cost-efficient
a
wide
range
priority
pollutants.
Frontiers in Chemistry,
Год журнала:
2022,
Номер
10
Опубликована: Сен. 30, 2022
Water
contamination
has
become
increasingly
a
critical
global
environmental
issue
that
threatens
human
and
ecosystems'
health.
Monitoring
risk
assessment
of
toxic
pollutants
in
water
bodies
is
essential
to
identifying
pollution
treatment
needs.
Compared
with
the
traditional
monitoring
approaches,
biosensing
via
whole-cell
bioreporters
(WCBs)
exhibited
excellent
capabilities
for
detecting
bioavailability
multiple
by
providing
fast,
simple,
versatile
economical
way
assessment.
The
performance
WCBs
determined
its
elements
construction,
such
as
host
strain,
regulatory
reporter
genes,
well
experimental
conditions.
Previously,
numerous
studies
have
focused
on
design
construction
WCB
rather
than
improving
detection
process
commercialization
this
technology.
For
investigators
working
field,
can
be
used
detect
more
important
how
they
are
constructed.
This
work
provides
review
development
brief
introduction
genetic
strategies
aims
summarize
key
application
technology
contaminants,
including
organic
heavy
metals.
In
addition,
current
status
highlighted.
