PubMed,
Год журнала:
2023,
Номер
37(1), С. 65 - 73
Опубликована: Янв. 15, 2023
To
investigate
the
neuroprotective
effect
of
conducting
hydrogel
loaded
with
tetramethylpyrazine
sustained-release
microparticles
(hereinafter
referred
to
as
"TGTP
hydrogel")
on
spinal
cord
injury
rats.Forty-eight
adult
female
Sprague
Dawley
rats
were
randomly
divided
into
4
groups:
sham
operation
group
(group
A),
model
B),
conductive
C),
and
TGTP
D),
12
in
each
group.
Only
laminectomy
was
performed
A,
complete
transection
groups
B,
C,
D.
Basso-Bettie-Bresnahan
(BBB)
score
used
evaluate
recovery
hind
limb
motor
function
before
modeling
at
1,
3,
7,
14,
28
days
after
modeling,
respectively.
At
sacrificed
for
luxol
fast
blue
(LFB)
staining
detect
myelin
regeneration.
Nissl
survival
neurons.
Immunohistochemical
expression
inflammation-related
factors
[nuclear
factor
кB
(NF-кB),
tumor
necrosis
α
(TNF-α),
interleukin
10
(IL-10)].
Immunofluorescence
Western
blot
neurofilament
200
(NF200).BBB
scores
A
significantly
better
than
those
other
three
all
time
points
(P<0.05);
14
there
no
significant
difference
BBB
between
C
D
(P>0.05),
but
that
B
(P<0.05).
LFB
showed
structure
neurons
intact,
integrity
number
C.
absorbency
(A)
value
NF-кB
TNF-α
lower
(P<0.05),
IL-10
higher
NF-κB
while
nerve
fibers
clear
fluorescence
intensity
high.
The
NF200
some
could
be
seen.
analysis
relative
highest,
(P<0.05).The
can
effectively
promote
injury,
its
mechanism
may
related
regulation
inflammatory
response.探讨携载川芎嗪缓释微粒导电水凝胶（以下简称“TGTP水凝胶”）对脊髓损伤大鼠的神经保护作用。.将48只成年雌性SD大鼠随机分为4组，假手术组（A组）、模型组（B组）、导电水凝胶组（C组）、TGTP水凝胶组（D组），每组12只。A组仅行椎板切除术，B～D组均制备脊髓完全横断大鼠模型。分别于造模前及造模后1、3、7、14、28
d采用BBB评分评估各组大鼠后肢运动功能恢复情况。造模后28
d处死动物取材行劳克坚牢蓝（luxol
blue，LFB）染色检测髓鞘再生情况；Nissl染色检测神经元存活情况；免疫组织化学染色评估炎症相关因子（NF-кB、TNF-α、IL-10）表达情况；免疫荧光染色和Western
blot评估神经丝蛋白200（neurofilament
200，NF200）表达。.造模后各时间点A组BBB评分均优于其余3组（P<0.05）；14、28
d
C、D组BBB评分差异无统计学意义（P>0.05），但D组BBB评分明显优于B组（P<0.05）。LFB染色与Nissl染色示，A组神经元及髓鞘结构完整，D组髓鞘完整性与神经元存活数量均明显优于B、C组。免疫组织化学染色示，A组NF-κB、TNF-α吸光度（A）值明显低于B、C组（P<0.05），IL-10
A值明显高于其余3组（P<0.05）；D组NF-κB
A值明显低于B、C组，TNF-α
A值明显低于B组，IL-10
A值明显高于B组，差异均有统计学意义（P<0.05）。免疫荧光染色示，A组神经元及神经纤维结构清晰，荧光强度高；D组较B、C组NF200荧光强度高，可见部分神经纤维。Western
blot检测示A组NF200蛋白相对表达量最高，D组NF200蛋白相对表达量明显高于B、C组（P<0.05）。.TGTP水凝胶能有效促进脊髓损伤大鼠运动功能恢复，其机制可能与调节炎症反应有关。.
Biomedicines,
Год журнала:
2022,
Номер
10(10), С. 2563 - 2563
Опубликована: Окт. 13, 2022
On
the
slow
path
to
improving
life
expectancy
and
quality
of
patients
post
spinal
cord
injury
(SCI),
recovery
remains
controversial.
The
potential
role
regenerative
capacity
nervous
system
has
led
numerous
attempts
stimulate
SCI
re-establish
interrupted
sensorimotor
loop
understand
its
in
process.
Numerous
resources
are
now
available,
from
pharmacological
biomolecular
approaches
neuromodulation
rehabilitation
interventions
based
on
use
various
neural
interfaces,
exoskeletons,
virtual
reality
applications.
integration
existing
seems
be
a
promising
field
research,
especially
perspective
living
conditions
short
medium
term.
Goals
such
as
reducing
chronic
forms
neuropathic
pain,
regaining
control
over
certain
physiological
activities,
enhancing
residual
abilities
often
more
urgent
than
complete
functional
recovery.
In
this
article,
we
provide
an
overview
latest
for
treatment
through
broad
phases
rehabilitation.
underlying
intention
work
is
introduce
neuroplasticity-based
multimodal
approach
promote
improve
after
SCI.
Nonetheless,
when
used
separately,
therapeutic
have
been
shown
modest
outcomes.
Biomedicines,
Год журнала:
2023,
Номер
11(5), С. 1436 - 1436
Опубликована: Май 12, 2023
This
study
explores
the
therapeutic
efficacy
of
heparin-based
hydrogel
micropatches
containing
human
adipose-derived
stem
cells
(hASCs)
in
treating
neuropathic
pain
caused
by
nerve
damage.
Our
results
showed
that
hASCs
exhibited
neuroregenerative
and
pain-relieving
effects
when
used
with
animal
model.
The
use
this
combination
also
produced
enhanced
cell
viability
regeneration.
We
conducted
various
neurological
behavioral
tests,
dynamic
plantar
histological
examinations,
neuroelectrophysiological
examinations
to
confirm
effect.
findings
suggest
approach
could
maximize
improve
quality
life
for
patients
suffering
from
pain.
Tissue Engineering Part A,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 18, 2024
Tissue
engineering
provides
a
path
forward
for
emerging
personalized
medicine
therapies
as
well
the
ability
to
bring
about
cures
diseases
or
chronic
injuries.
Traumatic
spinal
cord
injuries
(SCIs)
are
an
example
of
injury
in
which
no
cure
complete
functional
recovery
treatment
has
been
developed.
In
part,
this
due
complex
and
interconnected
nature
central
nervous
system
(CNS),
cellular
makeup,
its
extracellular
matrix
(ECM),
site
pathophysiology.
One
way
combat
SCI
create
tissue-engineered
scaffolds
that
replace
replenish
aspects
CNS
tissue/ECM
damaged
following
immediate
subsequent
immune
response.
This
can
be
achieved
by
employing
tissue-engineering
triad
consisting
cells,
biomaterial(s),
environmental
factors.
Stem
with
their
innate
proliferate
differentiate,
common
choice
therapies.
Natural
synthetic
biomaterials
have
tunable
characteristics
normally
used
scaffold
base.
Environmental
factors
range
from
drugs
growth
(GFs)
proteins,
depending
on
if
idea
would
stimulate
exogeneous
endogenous
cell
populations
just
simply
retain
cells
effective
transplantation.
For
regeneration
integration
SCI,
must
promote
neuroprotection
neuroplasticity.
Tissue-engineering
strategies
shown
benefits
including
neuronal
differentiation,
axonal
regeneration,
outgrowth,
into
native
cord,
partial
recovery.
Overall,
review
focuses
background
causes
so
difficult
treat,
individual
components
triad,
how
combinatorial
beneficial
toward
prospects
future
PubMed,
Год журнала:
2023,
Номер
37(1), С. 65 - 73
Опубликована: Янв. 15, 2023
To
investigate
the
neuroprotective
effect
of
conducting
hydrogel
loaded
with
tetramethylpyrazine
sustained-release
microparticles
(hereinafter
referred
to
as
"TGTP
hydrogel")
on
spinal
cord
injury
rats.Forty-eight
adult
female
Sprague
Dawley
rats
were
randomly
divided
into
4
groups:
sham
operation
group
(group
A),
model
B),
conductive
C),
and
TGTP
D),
12
in
each
group.
Only
laminectomy
was
performed
A,
complete
transection
groups
B,
C,
D.
Basso-Bettie-Bresnahan
(BBB)
score
used
evaluate
recovery
hind
limb
motor
function
before
modeling
at
1,
3,
7,
14,
28
days
after
modeling,
respectively.
At
sacrificed
for
luxol
fast
blue
(LFB)
staining
detect
myelin
regeneration.
Nissl
survival
neurons.
Immunohistochemical
expression
inflammation-related
factors
[nuclear
factor
кB
(NF-кB),
tumor
necrosis
α
(TNF-α),
interleukin
10
(IL-10)].
Immunofluorescence
Western
blot
neurofilament
200
(NF200).BBB
scores
A
significantly
better
than
those
other
three
all
time
points
(P<0.05);
14
there
no
significant
difference
BBB
between
C
D
(P>0.05),
but
that
B
(P<0.05).
LFB
showed
structure
neurons
intact,
integrity
number
C.
absorbency
(A)
value
NF-кB
TNF-α
lower
(P<0.05),
IL-10
higher
NF-κB
while
nerve
fibers
clear
fluorescence
intensity
high.
The
NF200
some
could
be
seen.
analysis
relative
highest,
(P<0.05).The
can
effectively
promote
injury,
its
mechanism
may
related
regulation
inflammatory
response.探讨携载川芎嗪缓释微粒导电水凝胶（以下简称“TGTP水凝胶”）对脊髓损伤大鼠的神经保护作用。.将48只成年雌性SD大鼠随机分为4组，假手术组（A组）、模型组（B组）、导电水凝胶组（C组）、TGTP水凝胶组（D组），每组12只。A组仅行椎板切除术，B～D组均制备脊髓完全横断大鼠模型。分别于造模前及造模后1、3、7、14、28
d采用BBB评分评估各组大鼠后肢运动功能恢复情况。造模后28
d处死动物取材行劳克坚牢蓝（luxol
blue，LFB）染色检测髓鞘再生情况；Nissl染色检测神经元存活情况；免疫组织化学染色评估炎症相关因子（NF-кB、TNF-α、IL-10）表达情况；免疫荧光染色和Western
blot评估神经丝蛋白200（neurofilament
200，NF200）表达。.造模后各时间点A组BBB评分均优于其余3组（P<0.05）；14、28
d
C、D组BBB评分差异无统计学意义（P>0.05），但D组BBB评分明显优于B组（P<0.05）。LFB染色与Nissl染色示，A组神经元及髓鞘结构完整，D组髓鞘完整性与神经元存活数量均明显优于B、C组。免疫组织化学染色示，A组NF-κB、TNF-α吸光度（A）值明显低于B、C组（P<0.05），IL-10
A值明显高于其余3组（P<0.05）；D组NF-κB
A值明显低于B、C组，TNF-α
A值明显低于B组，IL-10
A值明显高于B组，差异均有统计学意义（P<0.05）。免疫荧光染色示，A组神经元及神经纤维结构清晰，荧光强度高；D组较B、C组NF200荧光强度高，可见部分神经纤维。Western
blot检测示A组NF200蛋白相对表达量最高，D组NF200蛋白相对表达量明显高于B、C组（P<0.05）。.TGTP水凝胶能有效促进脊髓损伤大鼠运动功能恢复，其机制可能与调节炎症反应有关。.
