Опубликована: Янв. 1, 2024
Язык: Английский
ACS Omega, Год журнала: 2025, Номер 10(10), С. 9869 - 9889
Опубликована: Март 10, 2025
The limitation of animal models to imitate a therapeutic response in humans is key problem that challenges their use fundamental research. Organ-on-a-chip (OOC) devices, also called microphysiological systems (MPS), are devices containing lining living cells grown under dynamic flow recapitulate the important features human physiology and pathophysiology with high precision. Recent advances microfabrication tissue engineering techniques have led wide adoption OOC next-generation experimental platforms. This review presents comprehensive analysis systems, categorizing them by types (single-pass multipass), operational mechanisms (pumpless pump-driven), configurations (single-organ multiorgan systems), along respective advantages limitations. Furthermore, it explores integration qualitative quantitative assay techniques, providing comparative evaluation without sensor integration. aims fill essential knowledge gaps, driving progress development paving way for breakthroughs biomedical research, pharmaceutical innovation, engineering.
Язык: Английский
Процитировано
0
Rapid Communications in Mass Spectrometry, Год журнала: 2025, Номер 39(14)
Опубликована: Апрель 21, 2025
ABSTRACT Rationale To perform native mass spectrometry (MS) studies, there are a limited number of volatile and electrospray ionization (ESI)‐MS compatible solutions, such as ammonium bicarbonate acetate (AA). These solutions could induce the unfolding proteins due to formation CO 2 bubbles or induced acidification during ESI. Hence, it was important introduce buffer suitable preserve form while simulating physiological conditions. Methods The 4‐ethylmorpholinium/acetate (4EM/A) compared AA for analysis protein complexes with ranges from 5 103 kDa isoelectric points (pI) between 3 11. evaluations were conducted by comparing native‐MS profiles, CCS values, arrival time distributions (ATDs), bioactivities. human cardiac troponin complex (cTn complex) its subunit T (cTnT) analyzed proof applicability this challenging complexes. Results 4EM/A led lower charge states AA, supporting likelihood preserving folding nano‐ESI in high vacuum environment MS. Ion mobility measurements revealed that exhibit degree conformational variation suggesting enhanced stability potential retention natural‐like compactness. Additionally, testing impact on bioactivity, lysozyme showed increased biological activity relative highlighting buffer's real‐time assessment interaction kinetics bioactivity. enabled cTnT first time. Conclusion We introduced 4EM/A, pK 7.72/4.76, promising studies maintain bioactivity integrity.
Язык: Английский
Процитировано
0
Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(5), С. 166 - 166
Опубликована: Май 8, 2025
The rapid development of microfluidics has driven innovations in material engineering, particularly through its ability to precisely manipulate fluids and cells at microscopic scales. Microfluidic biomaterials, a cutting-edge interdisciplinary field integrating microfluidic technology with biomaterials science, are revolutionizing biomedical research. This review focuses on the functional design fabrication organ-on-a-chip (OoAC) platforms via 3D bioprinting, explores applications drug delivery, cell culture, tissue evaluates potential systems advancing personalized healthcare. We systematically analyze evolution materials—from silicon glass polymers paper—and highlight advantages bioprinting over traditional methods. Currently, despite significant advances medicine, challenges scalability, stability, clinical translation remain. future will depend combining dynamic design, developing hybrid strategies that combine molds bio-printed structures, using artificial intelligence monitor delivery or response real time. believe collaborations between materials micromachining, medicine accelerate into therapies high-throughput screening tools.
Язык: Английский
Процитировано
0
Annals of Medicine and Surgery, Год журнала: 2024, Номер unknown
Опубликована: Апрель 16, 2024
Introduction: Approximately 50 million people worldwide have epilepsy, with many not achieving seizure freedom. Organ-on-chip technology, which mimics organ-level physiology, could revolutionize drug development for epilepsy by replacing animal models in preclinical studies. Our goal is to determine if customized micro-physiological systems can lead tailored treatments epileptic patients. Materials and methods: A comprehensive literature search was conducted utilizing various databases, including PubMed, Ebscohost, Medline, the National Library of Medicine, using a predetermined strategy. We focused on articles that addressed role personalized individual responses discussed different types diagnosis, current treatment options. Additionally, explored components design considerations were reviewed identify challenges opportunities challenging cases. Results: The system offers more accurate cost-effective alternative traditional assessing effects, toxicities, disease mechanisms. Nevertheless, designing patient-specific presents critical considerations, integration analytical biosensors patient-derived cells, while addressing regulatory, material, biological complexities. Material selection, standardization, vascular systems, cost efficiency, real-time monitoring, ethical are also crucial successful use this technology development. Conclusion: future organ-on-chip holds great promise, potential integrate artificial intelligence machine learning
Язык: Английский
Процитировано
2
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12
Опубликована: Окт. 2, 2024
Our previous article entitled "Proteomics and its applications in breast cancer", proposed a Breast Cancer Continuum Concept (BCCC), including Cell as well Proteomic Concept. cancer-on-chip (BCoC), cancer liquid biopsy-on-chip (BCLBoC), metastasis-on-chip (BCMoC) models successfully recapitulate reproduce
Язык: Английский
Процитировано
1
Biomolecules, Год журнала: 2024, Номер 14(12), С. 1569 - 1569
Опубликована: Дек. 9, 2024
‘Organ-on-a-chip’ technology is a promising and rapidly evolving model in biological research. This innovative microfluidic cell culture device was created using microchip with continuously perfused chambers, populated by living cells arranged to replicate physiological processes at the tissue organ levels. By consolidating multicellular structures, tissue–tissue interfaces, physicochemical microenvironments, these microchips can key functions. They also enable high-resolution, real-time imaging analysis of biochemical, genetic, metabolic activities functional contexts. accelerate research into development, physiology disease etiology, therapeutic approaches, drug testing. It enables replication entire functions (e.g., liver-on-a-chip, hypothalamus–pituitary-on-a-chip) or creation models amyotrophic lateral sclerosis-on-a-chip, Parkinson’s disease-on-a-chip) specialized combining them an integrated system. allows for significant reduction number animals used experiments, high reproducibility results, possibility simultaneous use multiple types single model. However, its application requires equipment, advanced expertise, currently incurs costs. Additionally, achieving level standardization needed commercialization remains challenge this stage development.
Язык: Английский
Процитировано
1
Опубликована: Янв. 29, 2024
this paper gives a singular method for analyzing actual time-implemented applications in silicon-on-chip (SOC) architectures. The proposed technique uses mixture of analytical answers and hardware degree simulations to benefit insights on system-level performance. An in-depth dialogue the implementation testing approach is furnished, along with detailed description simulation outcomes. highlights ability combination simulators understand machine's behavior better attain more accurate estimation machine Numerous examples reveal effectiveness obtained from consequences. In end, identifies several capability areas destiny research.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0