Vaccines,
Год журнала:
2024,
Номер
12(11), С. 1282 - 1282
Опубликована: Ноя. 15, 2024
Milk-derived
extracellular
vesicles
(mEVs)
are
emerging
as
promising
therapeutic
candidates
due
to
their
unique
properties
and
versatile
functions.
These
play
a
crucial
role
in
immunomodulation
by
influencing
macrophage
differentiation
cytokine
production,
potentially
aiding
the
treatment
of
conditions
such
bone
loss,
fibrosis,
cancer.
mEVs
also
have
capacity
modulate
gut
microbiota
composition,
which
may
alleviate
symptoms
inflammatory
bowel
diseases
promote
intestinal
barrier
integrity.
Their
potential
drug
delivery
vehicles
is
significant,
enhancing
stability,
solubility,
bioavailability
anticancer
agents
while
supporting
wound
healing
reducing
inflammation.
Additionally,
bovine
exhibit
anti-aging
protect
skin
cells
from
UV
damage.
As
vaccine
platforms,
offer
advantages
including
biocompatibility,
antigen
protection,
ability
elicit
robust
immune
responses
through
targeted
specific
cells.
Despite
these
applications,
challenges
persist,
complex
roles
cancer,
effective
loading,
regulatory
hurdles,
need
for
standardized
production
methods.
Achieving
high
targeting
specificity
understanding
long-term
effects
mEV-based
therapies
essential
clinical
translation.
Ongoing
research
aims
optimize
mEV
methods,
enhance
capabilities,
conduct
rigorous
preclinical
studies.
By
addressing
challenges,
hold
revolutionize
development
delivery,
ultimately
improving
outcomes
across
various
medical
fields.
Bioengineering,
Год журнала:
2025,
Номер
12(1), С. 92 - 92
Опубликована: Янв. 20, 2025
Critical
limb
ischemia
(CLI)
poses
a
substantial
and
intricate
challenge
in
vascular
medicine,
necessitating
the
development
of
innovative
therapeutic
strategies
to
address
its
multifaceted
pathophysiology.
Conventional
revascularization
approaches
often
fail
adequately
complexity
CLI,
identification
alternative
methodologies.
This
review
explores
uncharted
territory
beyond
traditional
therapies,
focusing
on
potential
two
distinct
yet
interrelated
entities:
cell-derived
extracellular
vesicles
(EVs)
artificial
nanovesicles.
Cell-derived
EVs
are
small
membranous
structures
naturally
released
by
cells,
nanovesicles
artificially
engineered
nanosized
vesicles.
Both
these
represent
promising
avenues
for
intervention.
They
act
as
carriers
bioactive
cargo,
including
proteins,
nucleic
acids,
lipids,
that
can
modulate
cellular
responses
associated
with
ischemic
tissue
repair
angiogenesis.
also
assesses
evolving
landscape
CLI
through
unique
perspective
The
spans
spectrum
from
early
preclinical
investigations
latest
translational
advancements,
providing
comprehensive
overview
current
state
research
this
emerging
field.
These
groundbreaking
vesicle
therapies
hold
immense
revolutionizing
treatment
paradigms.
Pharmaceutics,
Год журнала:
2024,
Номер
16(4), С. 567 - 567
Опубликована: Апрель 21, 2024
Extracellular
vesicles
(EVs),
acting
as
inherent
nanocarriers
adept
at
transporting
a
range
of
different
biological
molecules
such
proteins,
lipids,
and
genetic
material,
exhibit
diverse
functions
within
the
gastroenteric
tract.
In
states
normal
health,
they
participate
in
upkeep
systemic
organ
homeostasis.
Conversely,
pathological
conditions,
significantly
contribute
to
pathogenesis
gastrointestinal
diseases
(GIDs).
Isolating
EVs
from
patients'
biofluids
facilitates
discovery
new
biomarkers
that
have
potential
offer
rapid,
cost-effective,
non-invasive
method
for
diagnosing
prognosing
specific
GIDs.
Furthermore,
demonstrate
considerable
therapeutic
naturally
targeted
physiological
carriers
intercellular
delivery
cargo
or
nanoscale
tools
engineered
specifically
regulate
physio-pathological
conditions
disease
progression.
Their
attributes
including
safety,
high
permeability,
stability,
biocompatibility,
low
immunogenicity,
homing/tropism
capabilities
their
promising
clinical
applications.
This
review
will
delve
into
various
examples
serving
context
GIDs,
highlighting
both
functional
structural
conditions.
The
versatile
advantageous
properties
position
them
candidates
innovative
strategies
advancing
personalized
medicine
approaches
tailored
tract,
addressing
Journal of Agriculture and Food Research,
Год журнала:
2024,
Номер
18, С. 101370 - 101370
Опубликована: Авг. 25, 2024
The
polyphenol
resveratrol
(RSV)
undergoes
extensive
phase
II
metabolism,
which
limits
its
bioavailability
and
bioactivity,
including
anticancer
effects.
Growing
preclinical
evidence
reinforces
milk-derived
exosomes
(EXOs)
as
promising
nanocarrier
drugs
bioactives
delivery
systems.
Herein,
to
overcome
the
mentioned
RSV's
drawbacks,
EXOs
were
loaded
with
RSV
(EXO-RSV)
evaluate
brain
in
Sprague-Dawley
rats
vitro
antiproliferative
effects
against
human
neuroblastoma
SH-SY5Y
glioblastoma
U-87MG
cancer
cells
compared
non-encapsulated
RSV.
Pharmacokinetic
analyses
perfused
brains
showed
detection
only
after
EXO-RSV
administration,
not
administration.
Encapsulation
also
resulted
lower
concentrations
of
II-derived
metabolites
circulation.
Additionally,
blood-brain
barrier
(BBB)
transport
assays
using
microvascular
endothelial
(HBMECs)
corroborated
that
encapsulation
enhanced
efficiency
protected
it
from
cellular
metabolism.
In
silico
phase-II
conjugates,
primarily
3-glucuronide,
capacity
than
cross
BBB.
Finally,
(47−750
nM)
an
increased
dose-dependent
efficacy
on
both
cell
lines
(10
μM),
was
ineffective
72
h
treatment.
Our
findings
suggest
protect
metabolism
enhance
activity.
Pharmaceutics,
Год журнала:
2025,
Номер
17(2), С. 238 - 238
Опубликована: Фев. 12, 2025
Metabolic
diseases
like
obesity
and
diabetes
are
on
the
rise,
therapies
with
biomacromolecules
(such
as
proteins,
peptides,
antibodies,
oligonucleotides)
play
a
crucial
role
in
their
treatment.
However,
these
drugs
traditionally
injected.
For
patients
chronic
(e.g.,
metabolic
diseases),
long-term
injections
accompanied
by
inconvenience
low
compliance.
Oral
administration
is
preferred,
but
delivery
of
challenging
due
to
gastrointestinal
barriers.
In
this
article,
we
introduce
available
biomacromolecule
for
treatment
diseases.
The
barriers
oral
drug
strategies
overcome
also
explored.
We
then
discuss
alleviating
defects,
including
glucose
metabolism,
lipid
energy
such
insulin,
glucagon-like
peptide-1
receptor
agonists,
proprotein
convertase
subtilisin/kexin
type
9
inhibitors,
fibroblast
growth
factor
21
analogues,
peptide
YY
analogues.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 21, 2025
Abstract
Maternal
consumption
of
a
high-fat
diet
(mHFD)
during
perinatal
life
(the
collective
prenatal
and
postnatal
periods)
influences
neonatal
development,
initiates
hypothalamic-pituitary-adrenal
(HPA)
axis
activation,
impacts
the
long-term
physiological
metabolic
health
offspring.
Milk-derived
extracellular
vesicles
(MEVs)
are
lipid-coated
nanovesicles
found
in
mammalian
milk
that
survive
intestinal
degradation
cross
complex
biological
barriers,
including
blood-brain
barrier.
MEVs
have
known
cytoprotective
activity
peripheral
organs;
however,
their
pro-survival
functions
response
to
chronic
pro-inflammation
stemming
from
early
nutrient
stress
remain
unknown
brain.
Further,
sex
differences
resulting
MEV
treatment
require
investigation,
as
male
female
neonates
illicit
variable
responses
stress.
We
investigated
whether
promote
heat
shock
(HSR),
principal
mechanism
responsible
for
refolding
or
degrading
misfolded
protein
aggregates
through
action
(HSP)
chaperones.
interaction
between
HSR
liver,
hypothalamus,
prefrontal
cortex
rats
exposed
mHFD
within
hyporesponsive
period
at
day
11.
robustly
modulated
with
largest
recorded
cortex.
Specifically,
cortex,
led
an
upregulation
main
transcription
factor
(HSF1),
while
downregulating
negative
regulators
HSF1
(Hsp70
Hsp90).
These
results
suggest
may
influence
outcomes
by
activating
HSF1-mediated
specific
manner
mHFD.
Biotechnology Reports,
Год журнала:
2025,
Номер
46, С. e00891 - e00891
Опубликована: Март 30, 2025
Crocins
are
potent
antioxidants
with
significant
therapeutic
potential,
exhibiting
anti-inflammatory,
anticancer,
and
antidepressant
properties.
The
ornamental
plant
Crocos-mia
x
crocosmiiflora
is
widely
cultivated
for
its
aromatic
vibrant
flowers.
In
this
study,
we
identified
unique
crocins
as
the
primary
pigments
responsible
flower's
coloration.
These
metabolites
predominantly
consist
of
molecules
eight
glucose
units,
followed
by
containing
six
seven
units.
To
elucidate
mo-lecular
mechanisms
underlying
crocin
biosynthesis
in
C.
crocosmiiflora,
tran-scriptomic
analysis
was
performed
to
identify
key
carotenoid
cleavage
dioxygenase
(CCD)
genes.
Using
Crocus
sativus
CsCCD2L
gene
a
bait,
CCD
transcript
from
transcriptome
data.
Phylogenetic
revealed
that
belongs
CCD2
subfamily,
it
designated
CroCCD2.
Functional
characterization
CroCCD2
carried
out
using
bacterial
expression
systems
Nicotiana
benthamiana
plants
virus-mediated
system.
experiments
demonstrated
efficiently
converts
precursor
zeaxanthin
into
crocetin,
intermediate
biosynthesis.
Furthermore,
investigated
bioactivity
discovered
their
anti-inflammatory
effects
depend
on
vehiculation
within
exosomes
or
liposomes.
This
suggests
transport
mechanism
critical
biological
activity
crocins.
Our
findings
highlight
specialization
subfamilies
monocots
dicots
provide
evidence
exosome-transported
study
establishes
foundation
further
research
metabolic
network
could
be
introduced
other
crop
produce
these
bioactive
apocarotenoids.
Advanced Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
Abstract
Extracellular
vesicles
(EVs)
are
nanoscale
luminal
vesicles,
which
play
an
important
role
in
intercellular
communication
through
surface
signaling
and
molecular
cargo
delivery
(proteins,
lipids,
nucleic
acids,
etc.).
Recently,
plant‐derived
extracellular
(PDVs)
containing
multiple
biological
activities
have
received
increasing
attention
due
to
their
better
biocompatibility
lower
cytotoxicity
healthy
tissues.
In
the
biomedical
field,
PDVs
employed
as
vehicles,
enabling
diverse
functionalities
engineering
modification
techniques.
Nonetheless,
there
certain
issues
with
study
of
PDVs,
such
lack
standardization
identification
isolation
criteria.
This
review
provides
a
quick
overview
biogenesis,
physicochemical
properties,
techniques,
applications
current
studies,
while
critically
analyzing
challenges
opportunities.
paper
is
expected
provide
some
theoretical
guidance
for
development
further
applications.
Exosomes
have
garnered
attention
as
a
potential
cell-free
therapy
for
inflammatory
diseases
due
to
their
immunomodulatory
and
anti-inflammatory
properties.
