Transgenic Eucalyptus urophylla × Eucalyptus grandis Superior Clone DH3229: Achieving Glyphosate Resistance through Genetic Modification DOI Creative Commons
Wang Xue-jun, Haoyu Wei, Shuang Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Abstract Eucalyptus species are known for their high productivity and adaptability, making them a reliable source of timber. However, hybrid eucalyptus, particularly in the initial months plantation, is susceptible to weed competition. Glyphosate most commonly used herbicide control eucalyptus plantations. To incorporate glyphosate resistance into cultivars (Eucalyptus urophylla × E. grandis DH3229), g10-epsps gene, driven by 35S promoter, was introduced using an efficient Agrobacterium-mediated genetic transformation method. Following PCR-based selection detection, six plants were confirmed have successfully integrated epsps gene genome at various stages. Realtime fluorescence quantitative PCR enzyme-linked immunosorbent assay further validated gene's integration expression all independent events. Additionally, transgenic demonstrated significant after being treated with 200-fold diluted Roundup solution. Physiological analysis revealed that maintaining lower levels shikimic acid crucial tolerance. The successful development herbicide-resistant elite clones presents potential future breeding programs may be directly applicable plantation field testing.

Язык: Английский

Endophytic Bacillus spp. of coffee plants (Coffea arabica L.) and its potential in the biocontrol of phytopathogenic fungi and Lepidoptera larvae DOI Creative Commons
Marcelo Tavares de Castro, Antônia Débora Camila de Lima Ferreira, Izabela Nunes do Nascimento

и другие.

Egyptian Journal of Biological Pest Control, Год журнала: 2025, Номер 35(1)

Опубликована: Фев. 21, 2025

Abstract Background Research with coffee ( Coffea arabica L., Rubiaceae) endophytic microorganisms demonstrates that there is a rich microbiota has diverse abilities to protect plants and act against phytopathogens. Therefore, the isolation knowledge of are important for future explorations aimed at using promising strains in controlling pests diseases promoting plant growth. The present work to: (i) isolate identify Bacillus from leaves C. plants; (ii) carry out morphological molecular characterization; (iii) evaluate mycelial inhibition capacity soil phytopathogens vitro; (iv) pathogenicity tested lepidopteran insect pests. Results As result, three species were isolated: B. mycoides, thuringiensis velezensi s. was virulent Spodoptera frugiperda, Helicoverpa armigera, Chrysodeixis includens Diatraea saccharalis showed 100% pest mortality, lethal concentration (LC 50 values) estimated: 6.78 × 10 3 spores/ml S. frugiperda ; 6.23 2 H. armigera 9.28 5.81 1 D. . In total, seven genes related insects found based on genome: cry1Ab24, cry1Bd2, cry2Aa9, cry2Ab41, cry1Ea7, cry1Ia37 Spp1Aa1. Furthermore, velezensis able inhibit growth phytopathogens: Fusarium oxysporum (49%) , Rhizoctonia solan i (72%) Macrophomina phaseolina (65%) provided above 50% mortality Regarding mycoides it not efficient inhibiting pathogens causing mortality. Conclusion Two ) bacteria isolated potential use biological control agriculture, high larvae laboratory thurigiensis More studies these two must be carried greenhouse field prove its beneficial properties.

Язык: Английский

Процитировано

1
Genetic Engineering for Crop Improvement Against Stresses and Future Agriculture DOI
Neeraj Kumar Dubey, Rakesh Srivastava, Manali Singh

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Transgenic Eucalyptus urophylla × Eucalyptus grandis superior clone DH3229: achieving glyphosate resistance through genetic modification DOI
Wang Xue-jun, Haoyu Wei, Shuang Zhang

и другие.

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2025, Номер 160(2)

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Addressing the UN sustainable development goals of woody plants through genetic engineering DOI
Rafael Urrea‐López, Andrés Gatica-Arias

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 158(3)

Опубликована: Авг. 22, 2024

Язык: Английский

Процитировано

1
Transgenic Eucalyptus urophylla × Eucalyptus grandis Superior Clone DH3229: Achieving Glyphosate Resistance through Genetic Modification DOI Creative Commons
Wang Xue-jun, Haoyu Wei, Shuang Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Abstract Eucalyptus species are known for their high productivity and adaptability, making them a reliable source of timber. However, hybrid eucalyptus, particularly in the initial months plantation, is susceptible to weed competition. Glyphosate most commonly used herbicide control eucalyptus plantations. To incorporate glyphosate resistance into cultivars (Eucalyptus urophylla × E. grandis DH3229), g10-epsps gene, driven by 35S promoter, was introduced using an efficient Agrobacterium-mediated genetic transformation method. Following PCR-based selection detection, six plants were confirmed have successfully integrated epsps gene genome at various stages. Realtime fluorescence quantitative PCR enzyme-linked immunosorbent assay further validated gene's integration expression all independent events. Additionally, transgenic demonstrated significant after being treated with 200-fold diluted Roundup solution. Physiological analysis revealed that maintaining lower levels shikimic acid crucial tolerance. The successful development herbicide-resistant elite clones presents potential future breeding programs may be directly applicable plantation field testing.

Язык: Английский

Процитировано

0