Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions
Biotechnology Advances,
Год журнала:
2025,
Номер
unknown, С. 108514 - 108514
Опубликована: Янв. 1, 2025
Язык: Английский
Recent advances in recombinant production of soluble proteins in E. coli
Microbial Cell Factories,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 16, 2025
E.
coli
still
remains
the
most
commonly
used
organism
to
produce
recombinant
proteins
in
research
labs.
This
condition
is
mirrored
by
attention
that
researchers
dedicate
understanding
biology
behind
protein
expression,
which
then
exploited
improve
effectiveness
of
technology.
effort
witnessed
an
impressive
number
publications,
and
this
review
aims
organize
relevant
novelties
proposed
recent
years.
The
examined
contributions
address
several
known
bottlenecks
related
expression
coli,
such
as
improved
glycosylation
pathways,
more
reliable
production
whose
folding
depends
on
formation
disulfide
bonds,
possibility
controlling
even
benefiting
from
aggregates
or
need
overcome
dependence
bacteria
antibiotics
during
bacterial
culture.
Nevertheless,
majority
published
papers
aimed
at
identifying
conditions
for
optimal
control
translation
process
achieve
maximal
yields
functional
exogenous
proteins.
Despite
community
commitment,
critical
question
what
really
metabolic
burden
how
it
affects
both
host
metabolism
elusive
because
some
experimental
results
are
contradictory.
contribution
offer
a
tool
orient
themselves
complexity.
new
capacities
offered
artificial
intelligence
tools
could
help
clarifying
issue,
but
training
phase
will
probably
require
systematic
approaches
collect
sufficiently
uniform
data.
Язык: Английский
Response of Escherichia coli to Acid Stress: Mechanisms and Applications—A Narrative Review
Microorganisms,
Год журнала:
2024,
Номер
12(9), С. 1774 - 1774
Опубликована: Авг. 28, 2024
Change
in
pH
growth
conditions
is
the
primary
stress
for
most
neutralophilic
bacteria,
including
model
microorganism
Язык: Английский
Overview of Phage Defense Systems in Bacteria and Their Applications
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13316 - 13316
Опубликована: Дек. 12, 2024
As
natural
parasites
of
bacteria,
phages
have
greatly
contributed
to
bacterial
evolution
owing
their
persistent
threat.
Diverse
phage
resistance
systems
been
developed
in
bacteria
during
the
coevolutionary
process
with
phages.
Conversely,
contamination
has
a
devastating
effect
on
microbial
fermentation,
resulting
fermentation
failure
and
substantial
economic
loss.
Accordingly,
defense
derived
from
can
be
employed
obtain
robust
phage-resistant
host
cells
that
overcome
threats
posed
by
bacteriophages
industrial
processes.
In
this
review,
diverse
mechanisms,
including
remarkable
research
progress
potential
applications,
are
systematically
summarized.
addition,
development
prospects
challenges
discussed.
This
review
provides
useful
reference
for
developing
bacteria.
Язык: Английский