Optogenetic control of mitochondrial aggregation and function
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 6, 2025
The
balance
of
mitochondrial
fission
and
fusion
plays
an
important
role
in
maintaining
the
stability
cellular
homeostasis.
Abnormal
fragmentation
have
been
shown
to
be
associated
with
oxidative
stress,
which
causes
a
variety
human
diseases
from
neurodegeneration
disease
cancer.
Therefore,
induction
aggregation
may
provide
alternative
approach
alleviate
these
conditions.
Here,
optogenetic-based
system
(Opto-MitoA)
developed,
is
based
on
CRY2clust/CIBN
light-sensitive
module.
Upon
blue
light
illumination,
CRY2clust
relocates
cytosol
mitochondria
where
it
induces
by
homo-oligomerization
CRY2clust-CIBN
hetero-dimerization.
Our
functional
experiments
demonstrate
that
Opto-MitoA-induced
potently
alleviates
niclosamide-caused
cell
dysfunction
ATP
production.
This
study
establishes
novel
strategy
regulate
dynamics
cells,
potential
therapy
for
treating
mitochondrial-related
diseases.
Язык: Английский
Photosynthetic Bacteria: Light-Responsive Biomaterials for Anti-Tumor Photodynamic Therapy
International Journal of Nanomedicine,
Год журнала:
2025,
Номер
Volume 20, С. 465 - 482
Опубликована: Янв. 1, 2025
Abstract:
Photodynamic
therapy
(PDT)
is
a
promising
noninvasive
tumor
treatment
modality
that
relies
on
generating
reactive
oxygen
species
(ROS)
and
requires
an
adequate
supply
to
the
target
tissue.
However,
hypoxia
common
feature
of
solid
tumors
profoundly
restricts
anti-tumor
efficacy
PDT.
In
recent
years,
scholars
have
focused
exploring
nanomaterial-based
strategies
for
supplementation
integrating
non-oxygen-consuming
approaches
overcome
hypoxic
limitations
Some
harnessed
photosynthetic
production
cyanobacteria
under
light
irradiation
engineered
them
as
carriers
photosensitizers
instead
inorganic
nanomaterials,
resulting
in
bacteria
(PSB)
attracting
significant
attention.
Recent
studies
shown
light-triggered
PSB
can
exhibit
additional
properties,
such
hydrogen
production,
ROS
generation,
photothermal
conversion,
facilitating
their
use
light-responsive
biomaterials
enhancing
Therefore,
understanding
provide
new
insights
ideas
future
research.
This
review
mainly
introduces
characteristics
research
PDT
enrich
our
knowledge
this
area.
Finally,
challenges
prospects
using
enhance
were
also
discussed.
Keywords:
bacteria,
PDT,
hypoxia,
cyanobacteria,
purple
PTT
Язык: Английский
Targeting tumor angiogenesis and metabolism with photodynamic nanomedicine
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
13
Опубликована: Апрель 1, 2025
Photodynamic
therapy
(PDT)
holds
considerable
promise
as
a
tumor
treatment
modality,
characterized
by
its
targeted
action,
compatibility
with
other
therapeutic
approaches,
and
non
-
invasive
features.
PDT
can
achieve
remarkable
spatiotemporal
precision
in
ablation
through
the
generation
of
reactive
oxygen
species
(ROS).
Nevertheless,
despite
potential
treatment,
encounters
multiple
challenges
practical
applications.
is
highly
dependent,
thus
effectiveness
be
markedly
influenced
hypoxia.
The
co-existence
abnormal
vasculature
metabolic
deregulation
gives
rise
to
hypoxic
microenvironment,
which
not
only
sustains
survival
but
also
undermines
efficacy
PDT.
Consequently,
targeting
angiogenesis
metabolism
essential
for
revitalizing
This
review
emphasizes
mechanisms
strategies
predominantly
concentrating
on
interfering
reprogramming
cell
metabolism.
Lastly,
outlining
future
perspectives
current
limitations
are
summarized.
could
provide
new
insights
methodologies
overcoming
associated
ultimately
advancing
field
Язык: Английский
Redefining cancer photodynamic therapy with gold nanoparticles
Photochemistry and Photobiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Despite
advancements
made
in
treatment
options,
cancer
continues
to
be
one
of
the
leading
causes
death
worldwide.
Photodynamic
therapy
(PDT)
has
gained
attention
as
a
minimally
invasive
and
highly
selective
option
for
cancer.
However,
challenges
due
hydrophobicity
photosensitizers
their
poor
tumor
selectivity
have
limited
use
therapy.
Recent
developments
nanotechnology,
particularly
gold
nanoparticles
(AuNPs),
help
overcome
these
challenges.
AuNPs
provide
stable
biocompatible
platform
deliver
photosensitizers,
improving
solubility,
stability,
ability
target
tumors
while
reducing
side
effects.
Functionalized
take
advantage
mechanisms
like
enhanced
permeability
retention
(EPR)
effect
active
targeting,
reactive
oxygen
species
(ROS)
production
overall
therapeutic
efficacy.
This
review
explores
innovations
AuNP-based
PDT
systems,
including
ligand-functionalized
nanoparticles,
bioresponsive
coatings,
theranostic
approaches
that
combine
imaging
with
By
delving
into
important
aspects
synthesis,
characterization,
functionalization,
we
show
how
improve
delivery
performance
photosensitizers.
For
instance,
systems
functionalized
prostate-specific
membrane
antigen
(PSMA)
shown
increased
precision
efficacy
vivo.
These
are
paving
way
more
targeted
safer
treatments,
establishing
promising
approach
developing
effective
oncological
therapies
greater
fewer
Язык: Английский
Harnessing Metal Nanoparticles: Revolutionizing Cancer Therapy Through Targeted Drug Delivery and Tumor Microenvironment Modulation
NANO,
Год журнала:
2024,
Номер
19(10)
Опубликована: Май 4, 2024
Cancer
remains
a
significant
global
health
burden,
claiming
countless
lives
annually
and
incurring
substantial
direct
indirect
economic
costs.
Despite
advancements
in
therapeutic
modalities,
conventional
cancer
treatments,
including
chemotherapy,
radiotherapy
surgery,
are
often
hampered
by
limitations
such
as
insufficient
efficacy,
dose-dependent
side
effects,
high
costs
patient
tolerability.
This
has
spurred
extensive
research
into
novel
approaches
for
this
life-threatening
disease.
Metal
nanoparticles
(MNPs)
have
emerged
promising
tools
therapy
due
to
their
unique
physicochemical
properties
spanning
the
past
two
decades.
Owing
size
similarity
biological
molecules,
MNPs
possess
inherent
ability
penetrate
cellular
barriers,
making
them
ideal
carriers
targeted
drug
delivery.
These
multifunctional
nanoplatforms
offer
distinct
advantages
over
therapeutics
enabling
delivery
of
anticancer
agents
specific
tumor
sites,
thereby
enhancing
efficacy
minimizing
systemic
effects.
Photodynamic
sonodynamic
represent
particularly
strategies
that
leverage
treatment.
review
delves
potential
applications
modulating
microenvironment,
paving
way
exploration
vast
rapidly
evolving
field
nanomedicine.
Язык: Английский
CO-delivery of paclitaxel and indocyanine green with Koliphore-elp35/folic acid nanoparticles for NIR-triggered chemo/photodynamic therapy in MCF-7 and MCF-10 cells
Journal of Radiation Research and Applied Sciences,
Год журнала:
2024,
Номер
17(4), С. 101086 - 101086
Опубликована: Авг. 27, 2024
Drug
delivery
with
photodynamic
therapy
may
improve
breast
cancer
treatment.
based
polymeric
nanoparticles,
especially
those
made
from
natural
polysaccharides,
are
promising
treatment
carriers
due
to
their
biocompatibility
and
potential.
This
study
involved
the
synthesis
of
Koliphore-elp35/Folic
Acid-conjugated
(KoliFA)
nanoparticles
for
concurrent
paclitaxel
photosensitizer
indocyanine
green
(ICG)
cells,
aiming
effectiveness
combined
chemotherapy
PDT
by
using
near-infrared
(NIR)
laser
activate
The
MCF-7
normal
MCF-10
cell
lines
were
exposed
nanoparticle
formulation.
MTT
assay
evaluated
cytotoxic
effects
established
IC
50
values.
result
showed
that
KoliFA
is
a
near
sphere-shaped
particle
average
size
core
390
nm
layer
FA
on
outer
side
its
increased
1372
after
loading
PTX,
zeta
potential
−12.5
mV
decreased
−11mV
loading.
cytotoxicity
KoliFA-PTX-ICG
in
cells
increased,
reducing
9.360
μg/ml
compared
this
decrease
suggests
effect
ICG
key
factor
improving
efficacy
increasing
ROS
generation
sensitivity
Flow
cytometry
indicated
apoptosis
+
NIR
laser.
demonstrated
could
be
platform
combination
chemo-photodynamic
Язык: Английский
Harnessing the power of targeted metal nanocarriers mediated photodynamic and photothermal therapy
Nanomedicine,
Год журнала:
2024,
Номер
unknown, С. 1 - 19
Опубликована: Ноя. 15, 2024
The
treatment
of
cancer
has
become
a
profoundly
intricate
procedure.
Traditional
methods,
including
chemotherapy,
surgery
and
radiotherapy,
have
been
utilized,
while
notable
progress
achieved
in
recent
years.
Among
targeted
therapies
for
cancer,
folic
acid
(FA)
conjugated
metal-based
nanoparticles
(NP)
emerged
as
an
innovative
strategy,
namely
photodynamic
therapy
(PDT)
photothermal
(PTT).
These
NP
exploit
the
strong
attraction
between
FA
folate
receptors,
which
are
excessively
produced
several
cells,
order
to
enable
precise
administration
improved
effectiveness
treatment.
During
PDT,
functionalized
with
used
photosensitizers
activated
by
light,
produce
reactive
oxygen
species
that
cause
cells
undergo
apoptosis.
Within
framework
PTT,
these
effectively
transform
light
energy
into
concentrated
heat,
specifically
targeting
destroying
tumor
cells.
This
review
examines
fundamental
mechanisms
improve
PDT
PTT
simultaneously
presenting
important
findings
demonstrate
FA-functionalized
MNP
laboratory
animal
models.
In
addition,
paper
also
discusses
problems
potential
directions
their
clinical
translation.
Язык: Английский