The influence of different crosslinking agents onto the physical properties, integration behavior and immune response of collagen-based barrier membranes
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 6, 2025
This
study
investigates
the
mechanical
properties
as
well
in
vitro
and
vivo
cyto-
biocompatibility
of
collagen
membranes
cross-linked
with
glutaraldehyde
(GA),
proanthocyanidins
(PC),
hexamethylendiisocyanate
(HMDI)
1-Ethyl-3-(3-dimethylaminopropyl)
carbodiimide/N-hydroxysuccinimide
(EC/NHS).
A
non-crosslinked
membrane
was
used
reference
control
(RF).
The
initial
cytotoxic
analyses
revealed
that
PC,
EC,
HMDI
crosslinked
were
cytocompatible,
while
GA
thus
selected
positive
further
study.
Cross-linking
enhances
tensile
strength
collagenase
resistance,
effectively
prolonging
membrane's
standing
time
vivo.
Using
(immune-)
histochemistry
histomorphometrical
analyses,
cellular
inflammatory
responses,
tissue
integration
vascularization
patterns
at
10-,
30-,
90-day
post-implantation
a
subcutaneous
implantation
model
rats
analyzed.
PC
elicited
mildest
cell
levels,
akin
to
RF
membrane,
other
groups
induced
an
M1-dominated
macrophage
response
numerous
multinucleated
giant
cells
throughout
period.
EC
maintained
structural
stability
up
30
days
post-implantation,
similar
group,
whereas
others
collapsed
prematurely.
Concurrent
collapse,
transmembrane
occurred
across
all
groups.
Histopathological
histomorphometry
results
reveal
intricate
interplay
populations
vascularization.
These
findings
offer
valuable
insights
into
pivotal
role
cross-linkers
modulating
responses
membranes.
Язык: Английский
Fabrication and characterization of hydroxyapatite substituted with Mg2+, Sr2+, and ampicillin for bone and antibacterial applications
Materials Science and Engineering B,
Год журнала:
2025,
Номер
316, С. 118131 - 118131
Опубликована: Фев. 18, 2025
Язык: Английский
Dose-dependent enhancement of in vitro osteogenic activity on strontium-decorated polyetheretherketone
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 24, 2025
Polyetheretherketone
(PEEK)
is
widely
used
in
orthopedic
and
dental
implants
due
to
its
excellent
mechanical
properties,
chemical
stability,
biocompatibility.
However,
inherently
bioinert
nature
makes
it
present
weak
osteogenic
activity,
which
greatly
restricts
clinical
adoption.
Herein,
strontium
(Sr)
incorporated
onto
the
surface
of
PEEK
using
mussel-inspired
polydopamine
coating
improve
activity.
X-ray
photoelectron
spectroscopy
ion
release
assay
results
confirm
that
different
concentrations
Sr
are
substrate
surfaces.
The
strontium-modified
samples
show
a
stable
35
days
detection.
Better
MC3T3-E1
pre-osteoblasts
adhesion,
spreading,
proliferation
can
be
observed
groups,
demonstrates
with
improved
compatibility.
boosted
activity
has
been
demonstrated
by
better
performed
ALP
extracellular
matrix
mineralization,
collagen
secretion,
remarkable
up-regulation
ALP,
OCN,
OPN,
Runx2,
Col-I,
BSP,
OSX
pre-osteoblasts.
Additionally,
exhibit
dose-dependent
enhancement
osteoblasts
compatibility
PEEK-Sr10
group
shows
best.
These
findings
indicate
strontium-decorated
promising
application
implants.
Язык: Английский