Classical and emerging approximations for the screening of antimicrobial peptide libraries
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown, С. 195 - 232
Опубликована: Янв. 1, 2025
Язык: Английский
Nanotheranostics Revolutionizing Gene Therapy: Emerging Applications in Gene Delivery Enhancement
Journal of Nanotheranostics,
Год журнала:
2025,
Номер
6(2), С. 10 - 10
Опубликована: Апрель 9, 2025
Nanotheranostics—where
nanoscale
materials
serve
both
diagnostic
and
therapeutic
functions—are
rapidly
transforming
gene
therapy
by
tackling
critical
delivery
challenges.
This
review
explores
the
design
engineering
of
various
nanoparticle
systems
(lipid-based,
polymeric,
inorganic,
hybrid)
to
enhance
stability,
targeting,
endosomal
escape
genetic
payloads.
We
discuss
how
real-time
imaging
capabilities
integrated
into
these
platforms
enable
precise
localization
controlled
release
genes,
improving
treatment
efficacy
while
reducing
off-target
effects.
Key
strategies
overcome
barriers
(such
as
proton
sponge
effect
photothermal
disruption)
achieve
nuclear
are
highlighted,
along
with
recent
advances
in
stimuli-responsive
that
facilitate
spatiotemporal
control
expression.
Clinical
trials
preclinical
studies
demonstrate
expanding
role
nanotheranostics
managing
cancer,
inherited
disorders,
cardiovascular
neurological
diseases.
further
address
regulatory
manufacturing
hurdles
must
be
for
widespread
clinical
adoption
nanoparticle-based
therapies.
By
synthesizing
progress
ongoing
challenges,
this
underscores
transformative
potential
effective,
targeted,
image-guided
delivery.
Язык: Английский
Enhancing Magnetic Micro- and Nanoparticle Separation with a Cost-Effective Microfluidic Device Fabricated by Laser Ablation of PMMA
Micromachines,
Год журнала:
2024,
Номер
15(8), С. 1057 - 1057
Опубликована: Авг. 22, 2024
Superparamagnetic
iron
oxide
micro-
and
nanoparticles
have
significant
applications
in
biomedical
chemical
engineering.
This
study
presents
the
development
evaluation
of
a
novel
low-cost
microfluidic
device
for
purification
hyperconcentration
these
magnetic
particles.
The
device,
fabricated
using
laser
ablation
polymethyl
methacrylate
(PMMA),
leverages
precise
control
over
fluid
dynamics
to
efficiently
separate
particles
from
non-magnetic
ones.
We
assessed
device’s
performance
through
Multiphysics
simulations
empirical
tests,
focusing
on
separation
magnetite
blue
carbon
dots
microparticles
polystyrene
at
various
total
flow
rates
(TFRs).
For
nanoparticle
separation,
achieved
recall
up
93.3
±
4%
precision
95.9
1.2%
an
optimal
TFR
2
mL/h,
significantly
outperforming
previous
models,
which
only
50%
recall.
Microparticle
demonstrated
accuracy
98.1
1%
mL/h
both
experimental
conditions.
Lagrangian
model
effectively
captured
microparticle
microparticles,
with
close
agreement
between
simulated
results.
Our
findings
underscore
robust
capability
distinguishing
nanoscales.
highlights
potential
low-cost,
non-cleanroom
manufacturing
techniques
produce
high-performance
devices,
thereby
expanding
their
accessibility
applicability
industrial
research
settings.
integration
continuous
magnet,
as
opposed
segmented
magnets
designs,
was
identified
key
factor
enhancing
efficiency.
Язык: Английский
A Novel Method for In Vivo Gene Editing in the Brain of Guppies Using Unique Nanoparticles as Delivery Vehicles
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Abstract
Background
Uncovering
the
genomic
basis
of
traits
has
advanced
rapidly
in
evolutionary
biology
and
neuroscience,
largely
through
phenotypic
with
adaptive
value
been
advancing
biology,
behavior,
due
to
research
using
non-traditional
model
systems.
Direct
gene
editing
adult
brain
represents
a
crucial
next
step
linking
genotype
phenotype,
avoiding
confounding
effects
that
arise
from
modifications
during
development.
However,
implementing
these
technologies
beyond
traditional
laboratory
models
remains
challenging
delivery
limitations.
Methods
We
developed
an
intracranial
microinjection
protocol
for
guppies
(
Poecilia
reticulata)
deliver
gene-editing
elements
cells.
designed
magnetic
nanoparticles
functionalized
novel
translocating
agent,
non-viral
carrier
capable
transporting
linearized
nucleic
acids
across
cellular
nuclear
membranes.
comprehensively
assessed
nanoparticle
uptake,
colocalization,
potential
health
impacts
histological
analysis,
liver
enzyme
activity
assays,
behavioral
assessments.
Results
Our
successfully
entered
cells
colocalized
nuclei
at
rates
exceeding
50%
after
two
weeks,
demonstrating
their
efficient
in
vivo
editing.
Health
assessments
showed
no
significant
cell
death
(>
80%
viability),
toxicity
(normal
ALT,
AST,
ALP
levels),
alterations
individual
social
behaviors,
confirming
nanoparticles’
biocompatibility
systemic
safety.
Conclusions
results,
combined
previous
vitro
work
our
are
effective
system
editing,
show
they
can
be
used
safe
interventions
P.
reticulata.
This
overcomes
major
technical
barrier
acid-carrying
vehicle
brain.
approach
provides
versatile
platform
studying
genetic
mechanisms
underlying
behavior
small
freshwater
fish
while
helping
overcome
limitations
conducting
functional
studies
on
non-model
organisms.
Язык: Английский
Magnetoliposomes for nanomedicine: synthesis, characterization, and applications in drug, gene, and peptide delivery
Expert Opinion on Drug Delivery,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 15, 2025
Magnetoliposomes
represent
a
transformative
advancement
in
nanomedicine
by
integrating
magnetic
nanoparticles
with
liposomal
structures,
creating
multifunctional
delivery
platforms
that
overcome
key
limitations
of
conventional
drug
carriers.
These
hybrid
systems
enable
precision
targeting
through
external
fields,
controlled
release
via
hyperthermia,
and
real-time
theranostic
capabilities,
offering
unprecedented
spatiotemporal
control
over
therapeutic
administration.
This
manuscript
focused
primarily
on
studies
from
2023-2025
however,
few
select
older
references
were
included
to
provide
background
context.This
review
examines
the
fundamental
design
principles
Magnetoliposomes,
including
bilayer
composition,
nanoparticle
integration
strategies,
physicochemical
properties
governing
their
biological
performance.
We
comprehensively
assess
synthesis
methodologies
-
traditional
thin-film
hydration
advanced
microfluidic
approaches
highlighting
impact
colloidal
stability,
encapsulation,
scaling
potential.
Characterization
techniques
essential
for
quality
regulatory
approval
are
systematically
reviewed,
followed
applications
across
oncology,
gene
delivery,
neurology,
infectious
disease
treatment,
supported
recent
experimental
evidence.
While
magnetoliposomes
show
remarkable
versatility,
clinical
translation
requires
addressing
biocompatibility
concerns,
manufacturing
scalability,
hurdles.
Integration
artificial
intelligence,
organ-on-chip
technologies,
personalized
medicine
will
likely
accelerate
development
toward
reality,
potentially
revolutionizing
treatment
paradigms
complex
diseases
tailored
interventions.
Язык: Английский
A mathematical phase field model predicts superparamagnetic nanoparticle accelerated fusion of HeLa spheroids for field guided biofabrication
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Июнь 5, 2025
In
vitro
tissue
models
are
crucial
for
regenerative
medicine,
drug
discovery,
and
the
reduction
of
animal
testing.
3D
bioprinting,
particularly
when
utilizing
magnetic
manipulation
cell
spheroids,
provides
precise
control
over
architecture.
However,
existing
mathematical
lack
precision
to
capture
interplay
between
biological
dynamics
forces
during
spheroid
fusion.
This
study
developed
validated
a
novel
model
that
simulates
magnetically
assisted
fusion,
taking
into
account
migration,
adhesion,
effects
external
fields.
The
integrates
principles
mechanics,
fluid
dynamics,
magnetostatics,
implemented
in
COMSOL
Multiphysics.
Experimental
validation
used
HeLa
spheroids
bioprinted
with
superparamagnetic
iron
oxide
nanoparticles
(SPIONs).
Spheroid
fusion
was
monitored
without
an
field
using
confocal
microscopy.
Rigorous
statistical
analysis
(MAE,
RMSE,
MAPE,
R²,
Chi-Square,
Bland-Altman,
variance-weighted
metrics)
evaluate
performance.
accurately
predicted
accelerated
under
manipulation,
reducing
time
from
approximately
7
days
(without
field)
2
days.
High
R²
values
(>
0.99
two-spheroid
>
0.97
multi-spheroid
systems)
narrow
confidence
intervals
demonstrated
strong
agreement
simulation
experiment.
Increased
system
complexity
introduced
slightly
higher
error
variability,
but
maintained
robust
predictive
capabilities.
disassembly
observed
four-spheroid
case,
highlighting
complex
cellular
reorganization.
validated,
high-precision
represents
significant
advancement
engineering,
providing
powerful
tool
optimizing
bioprinting
protocols,
designing
constructs,
advancing
development.
breakthrough
has
implications
medicine
discovery
while
also
importance
addressing
nanoparticle
safety
concerns.
Язык: Английский
Production and purification of outer membrane vesicles encapsulating green fluorescent protein from Escherichia coli: a step towards scalable OMV technologies
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 14, 2024
Outer
membrane
vesicles
(OMVs)
are
spherical
structures
that
contain
a
small
fraction
of
the
periplasm
Gram-negative
bacteria,
surrounded
by
its
outer
membrane.
They
naturally
produced
and
detached
from
bacterial
surface,
participate
in
diverse
biological
processes,
their
diameter
size
is
range
10–300
nm.
OMVs
have
gained
interest
different
applications,
such
as
development
biosensors,
vaccines,
protein
chips,
encapsulation
heterologous
proteins
peptides
expressed
these
microorganisms.
However,
use
applications
limited
due
to
low
yields
high
purification
costs.
In
this
study,
we
green
fluorescent
(GFP)
encapsulated
into
using
Escherichia
coli
JC8031
transformed
with
pTRC99A-ssTorA-GFP
establish
production
route.
Results
showed
motility
strain
prevents
immobilization
alginate,
which
hampers
OMVs.
To
address
issue,
zeolite-based
column
was
used
chromatographically
separate
smaller
particles.
Further
experiments
will
be
focused
on
standardizing
at
scalable
level.
Язык: Английский