Methods for Processing and Analyzing Images of Vascularized Micro-Organ and Tumor Systems DOI Creative Commons
Stephanie J. Hachey, Christopher J. Hatch,

Daniela Gaebler

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Our group has developed and validated an advanced microfluidic platform to improve preclinical modeling of healthy disease states, enabling extended culture detailed analysis tissue-engineered miniaturized organ constructs, or "organs-on-chips." Within this system, diverse cell types self-organize into perfused microvascular networks under dynamic flow within tissue chambers, effectively mimicking the structure function native tissues. This setup facilitates physiological intravascular delivery nutrients, immune cells, therapeutic agents, creates a realistic microenvironment study cellular interactions responses. Known as vascularized micro-organ (VMO), adaptable can be customized represent various systems tumors, forming micro-tumor (VMT) for cancer studies. The VMO/VMT system closely simulates in vivo nutrient exchange drug 3D microenvironment, establishing high-fidelity model screening mechanistic studies vascular biology, cancer, organ-specific pathologies. Furthermore, optical transparency device supports high-resolution, real-time imaging fluorescently labeled cells molecules construct, providing key insights responses, interactions, processes such epithelial-mesenchymal transition. To manage extensive data generated, we created standardized, high-throughput workflows image analysis. manuscript presents our processing pipeline, utilizing suite tools Fiji/ImageJ streamline extraction from model, substantially reducing manual time. Additionally, demonstrate how these adapted analyzing traditional vitro models microphysiological by other researchers.

Язык: Английский

Methods for Processing and Analyzing Images of Vascularized Micro-Organ and Tumor Systems DOI Creative Commons
Stephanie J. Hachey, Christopher J. Hatch,

Daniela Gaebler

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Our group has developed and validated an advanced microfluidic platform to improve preclinical modeling of healthy disease states, enabling extended culture detailed analysis tissue-engineered miniaturized organ constructs, or "organs-on-chips." Within this system, diverse cell types self-organize into perfused microvascular networks under dynamic flow within tissue chambers, effectively mimicking the structure function native tissues. This setup facilitates physiological intravascular delivery nutrients, immune cells, therapeutic agents, creates a realistic microenvironment study cellular interactions responses. Known as vascularized micro-organ (VMO), adaptable can be customized represent various systems tumors, forming micro-tumor (VMT) for cancer studies. The VMO/VMT system closely simulates in vivo nutrient exchange drug 3D microenvironment, establishing high-fidelity model screening mechanistic studies vascular biology, cancer, organ-specific pathologies. Furthermore, optical transparency device supports high-resolution, real-time imaging fluorescently labeled cells molecules construct, providing key insights responses, interactions, processes such epithelial-mesenchymal transition. To manage extensive data generated, we created standardized, high-throughput workflows image analysis. manuscript presents our processing pipeline, utilizing suite tools Fiji/ImageJ streamline extraction from model, substantially reducing manual time. Additionally, demonstrate how these adapted analyzing traditional vitro models microphysiological by other researchers.

Язык: Английский

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