Free Radical Biology and Medicine, Год журнала: 2021, Номер 175, С. 226 - 235
Опубликована: Сен. 5, 2021
Язык: Английский
Frontiers in Molecular Biosciences, Год журнала: 2022, Номер 8
Опубликована: Янв. 18, 2022
Background: Ferroptosis is a novel regulated cell death that characterized by iron-dependent oxidative damage. Renal cancer the second most common of urinary system, which highly correlated with iron metabolism. The aim our present study was to identify suitable ferroptosis-related prognosis signatures for renal cancer. Methods: We downloaded RNA-seq count data from Cancer Genome Atlas database and used DESeq2, Survival, Cox regression analyses screen signatures. Results: identified 5 differentially expressed lncRNAs (FR-DELs) (DOCK8-AS1, SNHG17, RUSC1-AS1, LINC02609, LUCAT1) be independently overall survival (OS) patients risk assessment model diagnosis constructed those FR-DELs could well predict outcome Conclusion: Our not only suggested as but also provided strategies other cancers in screening biomarkers.
Язык: Английский
Процитировано
22
Frontiers in Oncology, Год журнала: 2022, Номер 12
Опубликована: Окт. 31, 2022
The tumor immune microenvironment has been a research hot spot in recent years. cytokines and metabolites the can promote occurrence development of various ways help cells get rid surveillance system complete escape. Many studies have shown that existence is an important reason for failure immunotherapy. impact on systematic study. current this aspect may be only tip iceberg, relative lack integrity, related to heterogeneity tumor. This review mainly discusses status glucose metabolism lipid microenvironment, including phenotype microenvironment; effects these metabolic methods their three Impact: regulatory T (Tregs), tumor-associated macrophages (TAM), natural killer (NK cells); targeted At end article,the potential relationship between Ferroptosis years also briefly described.
Язык: Английский
Процитировано
21
Frontiers in Pharmacology, Год журнала: 2023, Номер 14
Опубликована: Фев. 15, 2023
Background: There is a rapid increase in lung adenocarcinomas (LUAD), and studies suggest associations between cuproptosis the occurrence of various types tumors. However, it remains unclear whether plays role LUAD prognosis. Methods: Dataset TCGA-LUAD was treated as training cohort, while validation cohort consisted merged datasets GSE29013, GSE30219, GSE31210, GSE37745, GSE50081. Ten studied cuproptosis-related genes (CRG) were used to generated CRG clusters cluster-related differential expressed gene (CRG-DEG) clusters. The differently lncRNA that with prognosis ability CRG-DEG put into LASSO regression for signature (CRLncSig). Kaplan-Meier estimator, Cox model, receiver operating characteristic (ROC), time-dependent AUC (tAUC), principal component analysis (PCA), nomogram predictor further deployed confirm model's accuracy. We examined connections other forms regulated cell death, including apoptosis, necroptosis, pyroptosis, ferroptosis. immunotherapy demonstrated by applying eight mainstream immunoinformatic algorithms, TMB, TIDE, immune checkpoints. evaluated potential drugs high risk CRLncSig LUADs. Real-time PCR human tissues performed verify expression pattern, signature's pan-cancer's also assessed. Results: A nine-lncRNA signature, CRLncSig, built owning prognostic power applied cohort. Each confirmed differentially real world real-time PCR. correlated 2,469/3,681 (67.07%) apoptosis-related genes, 13/20 (65.00%) necroptosis-related 35/50 (70.00%) pyroptosis-related 238/380 (62.63%) ferroptosis-related genes. Immunotherapy suggested status, checkpoints, KIR2DL3, IL10, IL2, CD40LG, SELP, BTLA, CD28, linked closely our potentially suitable targets. For those high-risk patients, we found three agents, gemcitabine, daunorubicin, nobiletin. Finally, some lncRNAs play vital cancer need more attention studies. Conclusion: results this study can help determine outcome effectiveness immunotherapy, well better select targets therapeutic agents.
Язык: Английский
Процитировано
12
Frontiers in Oncology, Год журнала: 2021, Номер 11
Опубликована: Дек. 2, 2021
Lung cancer is the second commonly diagnosed malignancy worldwide and has highest mortality rate among all cancers. Tremendous efforts have been made to develop novel strategies against lung cancer; however, overall survival of patients still low. Uncovering underlying molecular mechanisms this disease can open up new horizons for its treatment. Ferroptosis a newly discovered type programmed cell death that, in an iron-dependent manner, peroxidizes unsaturated phospholipids results accumulation radical oxygen species. Subsequent oxidative damage caused by ferroptosis contributes tumor cells. Therefore, understanding appears as promising strategy induce selectively. According evidence published now, significant numbers research done identify regulators cancer. review aims provide comprehensive standpoint address these molecules’ prognostic therapeutic values, hoping that road future studies field will be paved more efficiently.
Язык: Английский
Процитировано
27
Scientific Reports, Год журнала: 2022, Номер 12(1)
Опубликована: Янв. 24, 2022
Abstract Osteosarcoma (OS) is the most common type of primary malignant bone tumor. The high-throughput sequencing technology has shown potential abilities to illuminate pathogenic genes in OS. This study was designed find a powerful gene signature that can predict clinical outcomes. We selected OS cases with expression and survival data TARGET-OS dataset GSE21257 datasets as training cohort validation cohort, respectively. univariate Cox regression Kaplan–Meier analysis were conducted determine prognostic from cohort. These underwent LASSO regression, which then generated signature. harvested signature’s predictive ability further examined by analysis, receiver operating characteristic (ROC curve). More importantly, we listed similar studies recent year compared theirs ours. Finally, performed functional annotation, immune relevant correlation identification, infiltrating better he mechanism cells’ roles prognosis ability. A seventeen-gene ( UBE2L3, PLD3, SLC45A4, CLTC, CTNNBIP1, FBXL5, MKL2, SELPLG, C3orf14, WDR53, ZFP90, UHRF2, ARX, CORT, DDX26B, MYC, SLC16A3 ) regression. confirmed having strong stable capacity all studied cohorts several statistical methods. revealed superiority our after comparing it predecessors, GO KEGG annotations uncovered specifically action related Six signatures, including PRF1, CD8A, HAVCR2, LAG3, CD274, GZMA identified associating immune-infiltrating recognized vital T cells CD8 Mast activated, potentially support robust accurately prognosis. immunotherapy targets activated linked power.
Язык: Английский
Процитировано
18
Gut Microbes, Год журнала: 2025, Номер 17(1)
Опубликована: Апрель 13, 2025
Liver injury is an independent risk factor for multiple organ dysfunction and high mortality in patients with sepsis. However, the pathological mechanisms therapeutic strategies sepsis-associated liver have not been fully elucidated. Time-restricted feeding (TRF) a promising dietary regime, but its role septic remains unknown. Using 16S rRNA gene sequencing, Q200 targeted metabolomics, transcriptomics, germ-free mice, Hmgcs2/Lpin1 knockout Aml12 cells experiments, we revealed that TRF can mitigate by modulating gut microbiota, particularly increasing Lactobacillus murinus (L. murinus) abundance, which was significantly reduced mice. Further study live L. could markedly elevate serum levels of metabolite 3-hydroxybutyrate (3-HB) alleviate sepsis-related injury, while key enzyme 3-HB synthesis (3-hydroxy-3-methylglutaryl-CoA synthase 2, Hmgcs2) negated this protective effect. Additionally, were positively correlated abundance negatively indicators patients, demonstrating strong predictive value (AUC = 0.8429). Mechanistically, inhibited hepatocyte ferroptosis activating PI3K/AKT/mTOR/LPIN1 pathway, reducing ACSL4, MDA, LPO, Fe2+ levels. This demonstrates reduces microbiota to increase murinus, elevates activate inhibit ferroptosis. Overall, elucidates mechanism against identifies as potential target biomarker, thereby providing new insights into clinical management diagnosis injury.
Язык: Английский
Процитировано
0
Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)
Опубликована: Апрель 22, 2025
Язык: Английский
Процитировано
0
Biochimica et Biophysica Acta (BBA) - General Subjects, Год журнала: 2024, Номер 1868(11), С. 130706 - 130706
Опубликована: Авг. 23, 2024
Язык: Английский
Процитировано
3
Frontiers in Pharmacology, Год журнала: 2022, Номер 13
Опубликована: Окт. 3, 2022
Cuproptosis is a novel and unique cell death mode that has attracted significant interest in recent years. Little currently known about whether cuproptosis-related genes (CRGs) are associated with the pathophysiology survival of patients lung adenocarcinoma (LUAD). The present study sought to characterize transcriptional genetic alteration CRGs LUAD its potential significance tumor microenvironment predicting prognosis LUAD. secondary eventual aim was role immunotherapy response clinical value combined TNM stage. We found several CRGs, including FDX1, DLD, SLC31A1, MTF1, were enriched macrophages our single-cell RNA-seq data. Three distinct molecular subtypes identified correlated clinicopathological characteristics, prognosis, biological pathways, (TME) developed gene score (CRG_score) validated it three independent cohorts subtypes. low CRG_score group, characterized by greater immune score, immunophenoscore (IPS), lower dysfunction exclusion (TIDE) T-cell had better suggesting group responded more favorably immunotherapy, which anti-PD-1/L1 cohort (IMvigor210). In contrast, high sensitive targeted therapy chemotherapy, higher cancer stem (CSC) index half-maximal inhibitory concentration (IC50) for many drugs. Given established crosstalk between stage, we an accurate nomogram application CRG_score. Taken together, rigorous comprehensive examination their functions TME, drug sensitivity, prognosis. These findings improve current understanding cuproptosis LUAD, paving way assessment tailored treatment this patient population.
Язык: Английский
Процитировано
13
Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 9
Опубликована: Янв. 20, 2022
The Golgi apparatus (GA) is a cellular organelle that participates in the packaging, modification, and transport of proteins lipids from endoplasmic reticulum to be further fabricated before being presented other components. Recent studies have demonstrated GA facilitates numerous processes cancer development. Therefore, this study aimed establish novel lung adenocarcinoma (LUAD) risk evaluation model based on gene signatures. In study, we used TCGA-LUAD (n = 500) as training cohort GSE50081 127), GSE68465 (442), GSE72094 (398) validation cohorts. Two immunotherapy datasets (GSE135222 GSE126044) were also obtained previous study. Based machine algorithms bioinformatics methods, gene-related score (GARS) was established. We found GARS independently predicted prognosis LUAD patients remained effective across stages IA IIIA. Then, identified highly correlated with mutations P53 TTN. Further, related multiple immune microenvironmental characteristics. Furthermore, investigated GSE135222 GSE126044 lower may indicative an improved therapeutic effect PD-1/PD-L1 therapy. high lead better response anticancer drugs. Finally, established nomogram guide clinical application. To our knowledge, first demonstrate signature-based formula predict treatment patients.
Язык: Английский
Процитировано
12