c-Jun
is
a
proto-oncogene
functioning
as
transcription
factor
to
activate
gene
expression
under
many
physiological
and
pathological
conditions,
particularly
in
somatic
cells.
However,
its
role
early
embryonic
development
remains
unknown.
Here,
we
show
that
acts
one-way
valve
preserve
the
primed
state
impair
reversion
naïve
state.
induced
during
naive
transition,
it
works
stabilize
chromatin
structure
inhibit
reverse
transition.
Loss
of
has
surprisingly
little
effect
on
no
phenotypic
cells,
however,
cells
loss
leads
failure
correctly
close
naïve-specific
enhancers.
When
are
reprogram
state,
these
enhancers
more
rapidly
activated
when
lost
or
impaired,
conversion
efficient.
The
results
this
study
indicate
can
function
stabilizer
EpiSCs,
maintain
epigenetic
cell
type
act
for
fate
conversions.
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 28, 2025
Patients
and
healthcare
systems
face
significant
social
financial
challenges
due
to
the
increasing
number
of
individuals
with
chronic
external
internal
wounds
that
fail
heal.
The
complexity
healing
process
remains
a
serious
health
concern,
despite
effectiveness
conventional
wound
dressings
in
promoting
healing.
Recent
advancements
materials
science
fabrication
techniques
have
led
development
innovative
enhance
To
further
expedite
process,
novel
approaches
such
as
nanoparticles,
3D-printed
dressings,
biomolecule-infused
emerged,
along
cell-based
methods.
Additionally,
gene
therapy
technologies
are
being
harnessed
generate
stem
cell
derivatives
more
functional,
selective,
responsive
than
their
natural
counterparts.
This
review
highlights
potential
biomaterials,
3D
bioprinting,
gene-
therapies
However,
it
also
underscores
necessity
for
research
address
existing
integrate
these
strategies
into
standard
clinical
practice.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Ноя. 17, 2022
Abstract
The
thyroid
gland
captures
iodide
in
order
to
synthesize
hormones
that
act
on
almost
all
tissues
and
are
essential
for
normal
growth
metabolism.
Low
plasma
levels
of
lead
hypothyroidism,
which
is
one
the
most
common
disorder
humans
not
always
satisfactorily
treated
by
lifelong
hormone
replacement.
Therefore,
addition
lack
vitro
tractable
models
study
human
development,
differentiation
maturation,
functional
organoids
could
pave
way
explore
new
therapeutic
approaches.
Here
we
report
generation
transplantable
derived
from
embryonic
stem
cells
capable
restoring
athyreotic
mice
as
a
proof
concept
future
development.
Journal of Cell Science,
Год журнала:
2025,
Номер
138(9)
Опубликована: Май 1, 2025
As
we
have
learned
more
about
mitochondria
over
the
past
decades,
including
their
essential
cellular
roles
and
how
altered
mitochondrial
biology
results
in
disease,
it
has
become
apparent
that
they
are
not
just
powerplants
pumping
out
ATP
at
whim
of
cell.
Rather,
dynamic
information
energy
processors
play
crucial
directing
dozens
processes
behaviors.
They
provide
instructions
to
enact
programs
regulate
various
operations,
such
as
complex
metabolic
networks,
signaling
innate
immunity,
even
control
cell
fate,
dictating
when
cells
should
divide,
differentiate
or
die.
To
help
current
future
generations
biologists
incorporate
dynamic,
multifaceted
nature
assimilate
modern
discoveries
into
scientific
framework,
need
a
21st
century
'rebranding'.
In
this
Opinion
article,
argue
be
considered
'Chief
Executive
Organelle'
-
CEO
Global Change Biology,
Год журнала:
2021,
Номер
28(1), С. 54 - 71
Опубликована: Окт. 20, 2021
Rapid
climate
change
is
placing
many
marine
species
at
risk
of
local
extinction.
Recent
studies
show
that
epigenetic
mechanisms
(e.g.
DNA
methylation,
histone
modifications)
can
facilitate
both
within
and
transgenerational
plasticity
to
cope
with
changing
environments.
However,
reprogramming
(erasure
re-establishment
marks)
during
gamete
early
embryo
development
may
hinder
inheritance.
Most
our
knowledge
about
stems
from
mammals
model
organisms,
whereas
the
prevalence
extent
among
non-model
wild
populations
rarely
investigated.
Moreover,
whether
dynamics
are
sensitive
environmental
conditions
not
well
known,
representing
a
key
gap
in
pursuit
identify
underlying
links
between
parental
exposure
patterns
environmentally
adapted
offspring
phenotypes.
Here,
we
investigated
(DNA
methylation/hydroxymethylation)
gene
expression
across
gametogenesis
embryogenesis
stickleback
(Gasterosteus
aculeatus)
under
three
ocean
warming
scenarios
(ambient,
+1.5
+4°C).
We
found
acclimation
led
dynamic
temperature-sensitive
throughout
development.
Both
global
methylation/hydroxymethylation
genes
involved
modifications
were
strongly
differentially
affected
by
increased
scenarios.
Comparing
transcriptomic
profiles
gonads,
mature
gametes
embryonic
stages
showed
sex-specific
accumulation
temperature
sensitivity
several
actors.
methyltransferase
induction
was
primarily
maternally
inherited
(suggesting
maternal
control
remethylation),
histone-modifying
enzymes
shaped
parents.
Importantly,
massive,
temperature-specific
changes
landscape
occurred
blastula,
critical
stage
for
successful
development,
which
could,
thus,
translate
substantial
consequences
phenotype
resilience
In
summary,
study
identified
regulation,
reflecting
potential
'windows
opportunity'
adaptive
responses
future
change.
Nucleic Acids Research,
Год журнала:
2021,
Номер
49(16), С. 9132 - 9153
Опубликована: Авг. 3, 2021
Transposable
elements
(TEs)
occupy
nearly
40%
of
mammalian
genomes
and,
whilst
most
are
fragmentary
and
no
longer
capable
transposition,
they
can
nevertheless
contribute
to
cell
function.
TEs
within
genes
transcribed
by
RNA
polymerase
II
be
copied
as
parts
primary
transcripts;
however,
their
full
contribution
mature
transcript
sequences
remains
unresolved.
Here,
using
long
short
read
(LR
SR)
sequencing
data,
we
show
that
26%
coding
65%
noncoding
transcripts
in
human
pluripotent
stem
cells
(hPSCs)
contain
TE-derived
sequences.
Different
TE
families
incorporated
into
RNAs
unique
patterns,
with
consequences
structure
The
presence
a
is
correlated
TE-type
specific
changes
its
subcellular
distribution,
alterations
steady-state
levels
half-life,
differential
association
Binding
Proteins
(RBPs).
We
identify
hPSC-specific
incorporation
endogenous
retroviruses
(ERVs)
LINE:L1
protein-coding
mRNAs,
which
generate
sequence-derived
peptides.
Finally,
single
RNA-seq
reveals
hPSCs
express
ERV-containing
transcripts,
differentiating
subpopulations
lack
ERVs
SINE
LINE-containing
transcripts.
Overall,
our
comprehensive
analysis
demonstrates
the
more
widespread
has
greater
impact
than
previously
appreciated.
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
9
Опубликована: Янв. 11, 2022
Wound
healing
is
one
of
the
most
complex
physiological
regulation
mechanisms
human
body.
Stem
cell
technology
has
had
a
significant
impact
on
regenerative
medicine.
Adipose
stem
cells
(ASCs)
have
many
advantages,
including
their
ease
harvesting
and
high
yield,
rich
content
components
cytokines,
strong
practicability.
They
rapidly
become
favored
tool
in
Here,
we
summarize
mechanism
clinical
therapeutic
potential
ASCs
wound
repair.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(3), С. 2658 - 2658
Опубликована: Янв. 31, 2023
Complex
functioning
of
the
genome
in
cell
nucleus
is
controlled
at
different
levels:
(a)
DNA
base
sequence
containing
all
relevant
inherited
information;
(b)
epigenetic
pathways
consisting
protein
interactions
and
feedback
loops;
(c)
architecture
organization
activating
or
suppressing
genetic
between
parts
genome.
Most
research
so
far
has
shed
light
on
puzzle
pieces
these
levels.
This
article,
however,
attempts
an
integrative
approach
to
expression
regulation
incorporating
layers.
Under
environmental
stress
during
development,
differentiation
towards
specialized
types,
dysfunctional
tumor,
seems
react
as
a
whole
through
coordinated
changes
levels
control.
implies
need
for
framework
which
biological,
chemical,
physical
manifestations
can
serve
basis
coherent
theory
gene
self-organization.
An
international
symposium
held
Biomedical
Research
Study
Center
Riga,
Latvia,
25
July
2022
addressed
novel
aspects
abovementioned
topic.
The
present
article
reviews
most
recent
results
conclusions
state-of-the-art
this
multidisciplinary
field
science,
were
delivered
discussed
by
scholars
Riga
symposium.
Germline
epigenetic
programming,
including
genomic
imprinting,
substantially
influences
offspring
development.
Polycomb
Repressive
Complex
2
(PRC2)
plays
an
important
role
in
Histone
3
Lysine
27
trimethylation
(H3K27me3)-dependent
loss
of
which
leads
to
growth
and
developmental
changes
mouse
offspring.
In
this
study,
we
show
that
from
oocytes
lacking
the
PRC2
protein
Embryonic
Ectoderm
Development
(EED)
were
initially
developmentally
delayed,
characterised
by
low
blastocyst
cell
counts
substantial
delay
mid-gestation
embryos.
This
initial
was
resolved
as
underwent
accelerated
fetal
development
late
gestation
resulting
similar
stage
weight
controls
at
birth.
The
Eed
-null
associated
with
remodelling
placenta,
involved
increase
maternal
tissue
size,
conspicuous
expansion
glycogen-enriched
population,
delayed
parturition.
Despite
placental
development,
efficiency,
blood
glucose
levels
low,
metabolome
unchanged.
Moreover,
while
expression
H3K27me3-imprinted
gene
amino
acid
transporter
Slc38a4
increased,
individual
acids
controls,
indicating
transport
not
enhanced.
Genome-wide
analyses
identified
extensive
transcriptional
dysregulation
DNA
methylation
affected
placentas,
a
range
imprinted
non-imprinted
genes.
Together,
deletion
growing
resulted
hyperplasia,
our
data
indicate
remarkable
capacity
for
be
normalised
despite
inefficient
function
H3K27me3-dependent
imprinting.
