Infrared nanoimaging of neuronal ultrastructure and nanoparticle interaction with cells

Nanoscale, Год журнала: 2024, Номер 16(12), С. 6190 - 6198

Опубликована: Янв. 1, 2024

We use infrared nanoscopy to image subcellular components of hippocampal neurons in a chemistry specific way and study their interaction with therapeutic nanoparticles.

Язык: Английский

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Altering Cell Junctional Tension in Spheroids through E-Cadherin Engagement Modulation

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(6), С. 3766 - 3776

Опубликована: Май 10, 2024

Cadherin-mediated tension at adherens junctions (AJs) is fundamental for cell–cell adhesion and maintaining epithelial integrity. Despite the importance of manipulating AJs to dissect interactions, existing three-dimensional (3D) multicellular models have not adequately addressed precise manipulation these junctions. To fill this gap, we introduce E-cadherin-modified gauge tethers (TGTs) within spheroids. The system enables both quantification modulation junctional with specific DNA triggers. Using rupture-induced fluorescence, successfully measure mechanical forces in 3D Furthermore, mechanically strong TGTs can maintain normal E-cadherin-mediated adhesion. Employing toehold-mediated strand displacement allowed us disrupt E-cadherin-specific adhesion, consequently altering intracellular Our methodology offers a robust way manipulate mechanics spheroid models.

Язык: Английский

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Calpain Regulation and Dysregulation—Its Effects on the Intercalated Disk

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(14), С. 11726 - 11726

Опубликована: Июль 21, 2023

The intercalated disk is a cardiac specific structure composed of three main protein complexes—adherens junctions, desmosomes, and gap junctions—that work in concert to provide mechanical stability electrical synchronization the heart. Each substructure regulated through variety mechanisms including proteolysis. Calpain proteases, class cysteine proteases dependent on calcium for activation, have recently emerged as important regulators individual components. In this review, we will examine how homeostasis regulates normal calpain function. We also explore calpains modulate adherens junctions activity by targeting proteins, describe molecular dysregulation leads structural signaling defects within then changes affects cardiomyocytes, such underlie various heart diseases.

Язык: Английский

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Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 9, 2023

Differentiated transporting epithelial cells present an extensive apical array of microvilli - a "brush border" where neighboring are linked together by intermicrovillar adhesion complexes (IMACs) composed protocadherins CDHR2 and CDHR5. Although loss-of-function studies provide strong evidence that IMAC function is needed to build mature brush border, how the contributes stabilization accumulation nascent remains unclear. We found that, early in differentiation, surface exhibits marginal microvilli, characterized higher packing density relative medial regions surface. While highly dynamic sample multiple orientations over time, protrusions exhibit constrained motion maintain vertical orientation. Unexpectedly, we span junctional space contact on cells, mediated CDHR2/CDHR5. FRAP analysis indicated these transjunctional IMACs stable between which explains restricted zone. Finally, long-term live imaging revealed at cell margins consistently leads cell. Collectively, our findings suggest stabilized capture mechanism localized enabled formation IMACs. These results inform understanding specializations assembled diverse systems.

Язык: Английский

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Exploring the evolving function of soluble intercellular adhesion molecule-1 in junction dynamics during spermatogenesis

Frontiers in Endocrinology, Год журнала: 2024, Номер 14

Опубликована: Янв. 8, 2024

Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane glycoprotein expressed on immune, endothelial, and epithelial cells. Its ectodomain can be proteolytically cleaved to release circulating soluble form called sICAM-1. Clinical studies demonstrate sICAM-1 upregulated in various diseases associated with disease severity. Research has identified as regulator of the blood-testis barrier (BTB) spermatogenesis. Overexpression weakened BTB vitro vivo, downregulated junction proteins including N-cadherin, γ-catenin, connexin 43, caused germ cell loss. This contrasts barrier-strengthening effects membrane-bound ICAM-1. may act molecular switch enabling cells open Sertoli-germ for transport across seminiferous epithelium. While mechanism remains unclear, reduced SRC family kinase (SFK) signaling was observed following overexpression. promotes protein endocytosis degradation, influences cytoskeletal dynamics, affects polarity. As overexpression phenocopies inhibition, operate downstream regulating dynamics Investigating sICAM-1's structure-function regions targets will elucidate mechanisms disruption. knowledge could enable strategies targeting sICAM-1/SRC modulate permeability treat male infertility or involving endothelial/epithelial dysfunction.

Язык: Английский

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Too old for hide-and-seek: Cell maturation reveals hidden apical junctional organization

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(12)

Опубликована: Март 11, 2024

Язык: Английский

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Morphology of cells undergoing epithelial mesenchymal transition in microinvasive and early invasive oral squamous cell carcinom a – a light microscopic study

Polish Journal of Pathology, Год журнала: 2022, Номер 73(3), С. 244 - 254

Опубликована: Янв. 1, 2022

The present study focuses on identification of cancer attributes epithelial mesenchymal transition (EMT) at the earliest possible stage (microinvasion) under a light microscope by using hematoxylin and eosin stains, making it feasible for researchers to investigate such cases with ease without use extensive setups. is first in English literature define EMT features micro-invasive early invasive oral squamous cell carcinoma (OSCC) microscope. This retrospective histological sections 43 OSCC from Department Oral Pathology Microbiology. data collected were later statistically analyzed. A total 11 32 assessed core EMT. predominant feature defining found was dense inflammatory infiltrate both microinvasive (91%) (88%) followed individualization 82% 75% OSCC, which then other features. Reporting histopathological reports daily basis can aid diagnosis as well understanding carcinogenesis stages. Thereby, inclusion targeting therapeutics stages significantly alter prognosis cancer.

Язык: Английский

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ПЕРВЫЕ ДАННЫЕ О МИКРОМИНЕРАЛЬНЫХ ФОРМАХ НАХОЖДЕНИЯ ЭЛЕМЕНТОВ В ВОЛОСЯНОМ ПОКРОВЕ МЛЕКОПИТАЮЩИХ ПРИМОРСКОГО КРАЯ

Bulletin of the Tomsk Polytechnic University Geo Assets Engineering, Год журнала: 2023, Номер 334(2), С. 34 - 45

Опубликована: Фев. 5, 2023

Ссылка для цитирования: Первые данные о микроминеральных формах нахождения элементов в волосяном покрове млекопитающих Приморского Края /С.С. Ильенок, Н.В. Барановская, А.М, Паничев, И.В. Серёдкин, А.И. Беляновская, И.Ю. Чекрыжов, Р.А. Макаревич, Б.Р. Соктоев // Известия Томского политехнического университета. Инжиниринг георесурсов. – 2023. Т. 334. № 2. С. 34-45. Актуальность работы состоит необходимости изучения вариативности химического состава организма зависимости от геолого-геохимических факторов среды на территориях их обитания условиях нормального функционирования и при наличии патологических процессов. Выполненные исследования привносят новые знания понимание процессов миграции обширного набора химических из горных пород живые организмы. В рамках выполнения гранта РНФ изучен волосяной покров благородного оленя (Cervus elaphus) дикого кабана (Sus scrofа), добытых Тернейском районе края, территории, прилегающей к Сихотэ-Алинскому государственному биосферному заповеднику, а также домашней свиньи scrofa domesticus) частного подворья п. Терней. На некоторых участках территории заповедника развито явление геофагии среди диких копытных, следствием которого является формирование характерных ландшафтных комплексов кудуров. последние годы качестве причины все чаще рассматриваются редкоземельные элементы, причем возможен как дефицит организме, так избыток. Как следствие у животных может возникать необходимость либо пополнения редкоземельных элементов, избавления них. Источниками могут быть вода, кормовая растительность, коры выветривания (в Сихотэ-Алине это преимущественно риолиты, туфы туффиты). Химические включая поступлении организм накапливаются тканях организма, том числе покрове. Именно поэтому изучение включений вызывает интерес. Цель: поиск определение микроминералов с Тернейского района выявление мест локализации микроминералов. Методы. Образцы волосяного покрова изучались сканирующем электронном микроскопе Hitachi S-3400N энерго-дисперсионным спектрометром Bruker X@Flash 5010. Полученные результаты интерпретировались применением современных интернет-баз минералов. Результаты. Электронно-микроскопические показали, что содержатся алюмосиликаты сложного состава, оксиды меди железа, ильменит, кварц, галенит, мусковит, кальцит, калиевый полевой шпат, альбит, барит, циркон, пироксен, апатит, фосфаты редких земель. выявлены плагиоклаз, титанит монтмориллонит. обнаружены мусковит-иллит, каолинит, силикат титана, хлорит, титанит, рутил, никель самородный, сильвин, церия, Ce-Al фазы, комплексные фазы Si-Al-S-Na-O. При этом существенная часть перечисленных минералов обнаружена толще кутикулы волос всех животных, позволяет предполагать эндогенное происхождение (за счет поступления крови). Выводы. Изучение внутренних срезов поверхности выявило существование экзогенного, эндогенного накоплении виде минеральных включений.

Язык: Русский

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Imaging Techniques to Study Tight Junctions

Опубликована: Янв. 1, 2023

Tight junctions (TJs) are important regulators of paracellular permeability that act as protective barriers against pathogens and maintain cell polarity homeostasis by regulating the flow ions, solutes, macromolecules. TJs essential to barrier integrity epithelial endothelial cells, loss their function is linked various pathological conditions. To better understand molecular mechanisms underlying junctional architecture, it vital nanoscale organization components TJs, such occludins, adhesion molecules (JAMs), and, most importantly claudins. Molecular imaging techniques ultrasound, positron emission tomography (PET), single-photon computed (SPECT), magnetic resonance (MRI) enable visualization microstructures at a cellular level. In this chapter, we discuss significance modalities application in visualizing TJ diseases. We posit other complexes can provide insight into nanoarchitecture model organisms help study specificity therapeutic agents involved modulating permeability. Improved knowledge regulation using recognizing lead compromised lay groundwork for developing strategies target improved drug delivery with minimal toxicity facilitate development imaging-based biomarkers enhance mechanistic understanding dysfunction pathologies.

Язык: Английский

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Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Дек. 19, 2023

The manipulation of biological materials at cellular level constitutes a sine qua non and provocative research area regarding the development micro/nano‐medicine. In this study, we report on 3D superparamagnetic microcage‐like structures that, in conjunction with an externally applied static magnetic field, were highly efficient entrapping cells. fabricated using Laser Direct Writing via Two‐Photon Polymerization (LDW TPP) IP‐L780 biocompatible photopolymer/iron oxide nanoparticles (MNPs) composite. unique properties LDW TPP technique enabled reproduction complex architecture structures, very high accuracy i.e., about 90 nm lateral resolution. hyperspectral microscopy was employed to investigate structural compositional characteristics structures. Scanning Electron Microscopy coupled Energy Dispersive X‐Ray Spectroscopy used prove features morphology functionality seeded MG‐63 osteoblast‐like Comparative studies made photopolymer alone (i.e., without properties). We found that cell‐seeded by IP‐L780/MNPs composite actuated fields 1.3 T 13.66 ± 5.11 folds ( p < 0.01) more terms cells entrapment than could not be magnetically). their actuation external acted synergy for cells, showing significant potential bone tissue engineering applications.

Язык: Английский

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