The sodium/ascorbic acid co-transporter SVCT2 distributes in a striated membrane-enriched domain at the M-band level in slow-twitch skeletal muscle fibers DOI Creative Commons

Daniel Sandoval,

Jessica Mella, Jorge Ojeda

и другие.

Biological Research, Год журнала: 2024, Номер 57(1)

Опубликована: Ноя. 6, 2024

Abstract Background Vitamin C plays key roles in cellular homeostasis, functioning as a potent antioxidant and positive regulator of cell differentiation. In skeletal muscle, the vitamin C/sodium co-transporter SVCT2 is preferentially expressed oxidative slow fibers. up-regulated during early fusion primary myoblasts decreases initial myotube growth, indicating relevance uptake via for muscle differentiation fiber-type definition. However, our understanding expression function adult muscles still limited. Results this study, we demonstrate that exhibits an intracellular distribution chicken muscles, following highly organized striated pattern. A similar was observed human samples, cultured myotubes, isolated mouse myofibers. Immunohistochemical analyses, combined with biochemical fractionation experiments, reveal strong co-localization detergent-soluble membrane fractions at central sarcomeric M-band, where it co-solubilizes sarcoplasmic reticulum proteins. Remarkably, electrical stimulation myofibers induces redistribution into vesicular Conclusions Our results provide novel insights dynamic different compartments response to functional demands.

Язык: Английский

Loss of popdc3 Impairs Mitochondrial Function and Causes Skeletal Muscle Atrophy and Reduced Swimming Ability in Zebrafish DOI Creative Commons

Chen‐Chen Sun,

Zhanglin Chen, Dong Kwon Yang

и другие.

Journal of Cachexia Sarcopenia and Muscle, Год журнала: 2025, Номер 16(2)

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Muscle-Derived Mitochondria as a Novel Therapy for Muscle Degeneration After Rotator Cuff Tears DOI
Ding Xu,

Qing Liu,

Jingzeng Wang

и другие.

Journal of Bone and Joint Surgery, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Background: Rotator cuff tears (RCTs) commonly lead to muscle atrophy, fatty infiltration, and fibrosis, resulting in pain, weakness, impaired shoulder mobility. These pathological changes are often irreversible pose substantial treatment challenges. The aim of this study was evaluate the therapeutic potential muscle-derived mitochondria (Mito) mitigating degeneration fibrosis following RCTs. Methods: Sprague Dawley rats were assigned 3 groups: sham surgery, RCTs treated with Mito, or phosphate-buffered saline solution (PBS). Following RCTs, vivo Mito PBS treatments administered supraspinatus muscles (SSPs) immediately then biweekly for 12 weeks. Data collected on morphology, oxidative stress, mitochondrial function, macrophage phenotypes, serum inflammatory cytokines. In vitro experiments included tracking bone marrow–derived macrophages (BMDMs), characterization polarization, cytokine profiling. Results: Isolated preserved their morphology function. improved wet weight (p < 0.0001) fiber cross-sectional area while reducing infiltration 0.0001). It upregulated markers cytochrome c oxidase (COX IV) translocase outer membrane 20 (TOMM20) enhanced antioxidative activity, as shown by increased superoxide dismutase (SOD) activity 0.0001), elevated glutathione peroxidase (GSH-PX) levels = 0.038), decreased malondialdehyde (MDA) 0.0002). Mitochondrial density restored SSPs after treatment. Additionally, induced an anti-inflammatory phenotype reduced pro-inflammatory cytokines vitro. Conclusions: mitigated degeneration, fostered environment through modulation, demonstrating its a cell-free strategy RCT-related pathologies. Clinical Relevance: Although is preclinical study, approach offers novel avenue improving RCT outcomes. However, further validation large animal models needed address translational applicability these findings, given inherent regenerative capacity rodent muscles.

Язык: Английский

Процитировано

0
New perspective on mechanism of L-arginine in promoting skeletal muscle growth of grass carp (Ctenopharyngodon Idella): Via enhancing skeletal muscle functions and regulating energy metabolism DOI
Qiuyan Chen, Yulong Ma,

Wei‐Dan Jiang

и другие.

Aquaculture, Год журнала: 2025, Номер unknown, С. 742636 - 742636

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Evaluation of muscle quality and movement-related traits in two common carp strains under a rice–fish farming system DOI

Yating Gong,

Mingkun Luo,

Xianbo Zhang

и другие.

Aquaculture International, Год журнала: 2025, Номер 33(4)

Опубликована: Май 2, 2025

Язык: Английский

Процитировано

0
Enhanced Breast Cancer Treatment Using Phototherapy and RNS Therapy with Macrophage Membrane-Coated Liposomes DOI Creative Commons
Feiran Wang, Chenhao Li, Xiaodong Xu

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2024, Номер 239, С. 113961 - 113961

Опубликована: Май 11, 2024

Breast cancer, the predominant malignancy afflicting women, continues to pose formidable challenges despite advancements in therapeutic interventions. This study elucidates potential of phototherapy, comprising both photothermal and photodynamic therapy (PTT/PDT), as a novel promising modality. To achieve this goal, we devised liposomes coated with macrophage cell membranes including macrophage-associated membrane proteins, which have demonstrated promise biomimetic delivery systems for targeting tumors while preserving their inherent tumor-homing capabilities. integrated system comprised IR780, NONOate, perfluorocarbon. strategic encapsulation aims synergistic combination (PDT) reactive nitrogen species (RNS) therapy. Under near-infrared laser irradiation at 808 nm, IR780 demonstrates its ability prolifically generate oxygen (ROS), superoxide anion (O

Язык: Английский

Процитировано

3
Antibacterial activity of SnO2 in visible light enhanced by erbium–cobalt co-doping DOI

Xingkun Liang,

Rong Dai,

Qisheng Wang

и другие.

Colloids and Surfaces A Physicochemical and Engineering Aspects, Год журнала: 2023, Номер 676, С. 132257 - 132257

Опубликована: Авг. 18, 2023

Язык: Английский

Процитировано

6
Regimen on Dnaja3 haploinsufficiency mediated sarcopenic obesity with imbalanced mitochondrial homeostasis and lipid metabolism DOI Creative Commons

Yu‐Ning Fann,

Wan‐Huai Teo,

Hsin‐Chen Lee

и другие.

Journal of Cachexia Sarcopenia and Muscle, Год журнала: 2024, Номер 15(5), С. 2013 - 2029

Опубликована: Авг. 12, 2024

Sarcopenic obesity is characterized by excess fat mass and diminished muscular mass/function. DNAJA3, a mitochondrial co-chaperone protein, plays crucial role in skeletal muscle development. GMI, an immunomodulatory promotes myogenic differentiation through DNAJA3 activation. This study aims to elucidate the physiological effects of Dnaja3 haploinsufficiency on dysfunction dysregulated lipid metabolism assess efficacy GMI rescuing sarcopenic both vitro vivo.

Язык: Английский

Процитировано

1
Unveiling Cellular Secrets: Illuminating Carbon Dot Lighthouses for Improved Mitochondrial Exploration DOI

Sathyaprakash Kumarasamy,

Vinay Sharma

Chemistry of Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 4, 2024

Язык: Английский

Процитировано

1
Research on the Application of Isotope Ratio Mass Spectrometry Technology in Traceability Detection of Stimulants DOI Open Access

Yue Zhuo,

Yirang Wang,

Xiaomeng Xiang

и другие.

Journal of engineering system., Год журнала: 2024, Номер 2(1), С. 75 - 82

Опубликована: Март 1, 2024

Endogenous substances, which are produced by the body itself, have become a focus of concern due to their potential abuse as stimulants in sports. The chemical structure and physicochemical properties externally synthesized endogenous substances similar those internally, making them difficult detect. In response this challenge, it is crucial develop effective methods for detecting both exogenous substances. One promising approach leverages differences stable carbon isotope abundance between internal external sources, leading changes Compound-Specific Isotope Ratio (CIR) after ingestion. GC-C-IRMS method has emerged commonly used highly accurate technique tracing origin Recognizing its significance fight against doping, World Anti-Doping Agency (WADA) standardized established relevant benchmarks. This review article examines advancements made over past decade S1 anabolic steroids, S4 hormones metabolic modulators, S9 glucocorticoids. By offering comprehensive overview progress detection using ratio mass spectrometry, aim provide valuable insights enhancing existing anti-doping measures. research serves critical reference point ongoing efforts combat misuse realm

Язык: Английский

Процитировано

0
A meta-analysis of the efficacy of physical exercise interventions on activities of daily living in patients with Alzheimer's disease DOI Creative Commons

Yang Xiao,

Yu Fan, Ziding Feng

и другие.

Frontiers in Public Health, Год журнала: 2024, Номер 12

Опубликована: Ноя. 27, 2024

This study aimed to systematically review published randomized controlled trials on the effects of physical exercise activities daily living (ADL) in Alzheimer's patients through meta-analysis, thereby synthesizing existing evidence provide scientific intervention recommendations for prescriptions patients.

Язык: Английский

Процитировано

0