Biological Research,
Год журнала:
2024,
Номер
57(1)
Опубликована: Ноя. 6, 2024
Abstract
Background
Vitamin
C
plays
key
roles
in
cellular
homeostasis,
functioning
as
a
potent
antioxidant
and
positive
regulator
of
cell
differentiation.
In
skeletal
muscle,
the
vitamin
C/sodium
co-transporter
SVCT2
is
preferentially
expressed
oxidative
slow
fibers.
up-regulated
during
early
fusion
primary
myoblasts
decreases
initial
myotube
growth,
indicating
relevance
uptake
via
for
muscle
differentiation
fiber-type
definition.
However,
our
understanding
expression
function
adult
muscles
still
limited.
Results
this
study,
we
demonstrate
that
exhibits
an
intracellular
distribution
chicken
muscles,
following
highly
organized
striated
pattern.
A
similar
was
observed
human
samples,
cultured
myotubes,
isolated
mouse
myofibers.
Immunohistochemical
analyses,
combined
with
biochemical
fractionation
experiments,
reveal
strong
co-localization
detergent-soluble
membrane
fractions
at
central
sarcomeric
M-band,
where
it
co-solubilizes
sarcoplasmic
reticulum
proteins.
Remarkably,
electrical
stimulation
myofibers
induces
redistribution
into
vesicular
Conclusions
Our
results
provide
novel
insights
dynamic
different
compartments
response
to
functional
demands.
Journal of Bone and Joint Surgery,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Background:
Rotator
cuff
tears
(RCTs)
commonly
lead
to
muscle
atrophy,
fatty
infiltration,
and
fibrosis,
resulting
in
pain,
weakness,
impaired
shoulder
mobility.
These
pathological
changes
are
often
irreversible
pose
substantial
treatment
challenges.
The
aim
of
this
study
was
evaluate
the
therapeutic
potential
muscle-derived
mitochondria
(Mito)
mitigating
degeneration
fibrosis
following
RCTs.
Methods:
Sprague
Dawley
rats
were
assigned
3
groups:
sham
surgery,
RCTs
treated
with
Mito,
or
phosphate-buffered
saline
solution
(PBS).
Following
RCTs,
vivo
Mito
PBS
treatments
administered
supraspinatus
muscles
(SSPs)
immediately
then
biweekly
for
12
weeks.
Data
collected
on
morphology,
oxidative
stress,
mitochondrial
function,
macrophage
phenotypes,
serum
inflammatory
cytokines.
In
vitro
experiments
included
tracking
bone
marrow–derived
macrophages
(BMDMs),
characterization
polarization,
cytokine
profiling.
Results:
Isolated
preserved
their
morphology
function.
improved
wet
weight
(p
<
0.0001)
fiber
cross-sectional
area
while
reducing
infiltration
0.0001).
It
upregulated
markers
cytochrome
c
oxidase
(COX
IV)
translocase
outer
membrane
20
(TOMM20)
enhanced
antioxidative
activity,
as
shown
by
increased
superoxide
dismutase
(SOD)
activity
0.0001),
elevated
glutathione
peroxidase
(GSH-PX)
levels
=
0.038),
decreased
malondialdehyde
(MDA)
0.0002).
Mitochondrial
density
restored
SSPs
after
treatment.
Additionally,
induced
an
anti-inflammatory
phenotype
reduced
pro-inflammatory
cytokines
vitro.
Conclusions:
mitigated
degeneration,
fostered
environment
through
modulation,
demonstrating
its
a
cell-free
strategy
RCT-related
pathologies.
Clinical
Relevance:
Although
is
preclinical
study,
approach
offers
novel
avenue
improving
RCT
outcomes.
However,
further
validation
large
animal
models
needed
address
translational
applicability
these
findings,
given
inherent
regenerative
capacity
rodent
muscles.
Colloids and Surfaces B Biointerfaces,
Год журнала:
2024,
Номер
239, С. 113961 - 113961
Опубликована: Май 11, 2024
Breast
cancer,
the
predominant
malignancy
afflicting
women,
continues
to
pose
formidable
challenges
despite
advancements
in
therapeutic
interventions.
This
study
elucidates
potential
of
phototherapy,
comprising
both
photothermal
and
photodynamic
therapy
(PTT/PDT),
as
a
novel
promising
modality.
To
achieve
this
goal,
we
devised
liposomes
coated
with
macrophage
cell
membranes
including
macrophage-associated
membrane
proteins,
which
have
demonstrated
promise
biomimetic
delivery
systems
for
targeting
tumors
while
preserving
their
inherent
tumor-homing
capabilities.
integrated
system
comprised
IR780,
NONOate,
perfluorocarbon.
strategic
encapsulation
aims
synergistic
combination
(PDT)
reactive
nitrogen
species
(RNS)
therapy.
Under
near-infrared
laser
irradiation
at
808
nm,
IR780
demonstrates
its
ability
prolifically
generate
oxygen
(ROS),
superoxide
anion
(O
Journal of Cachexia Sarcopenia and Muscle,
Год журнала:
2024,
Номер
15(5), С. 2013 - 2029
Опубликована: Авг. 12, 2024
Sarcopenic
obesity
is
characterized
by
excess
fat
mass
and
diminished
muscular
mass/function.
DNAJA3,
a
mitochondrial
co-chaperone
protein,
plays
crucial
role
in
skeletal
muscle
development.
GMI,
an
immunomodulatory
promotes
myogenic
differentiation
through
DNAJA3
activation.
This
study
aims
to
elucidate
the
physiological
effects
of
Dnaja3
haploinsufficiency
on
dysfunction
dysregulated
lipid
metabolism
assess
efficacy
GMI
rescuing
sarcopenic
both
vitro
vivo.
Journal of engineering system.,
Год журнала:
2024,
Номер
2(1), С. 75 - 82
Опубликована: Март 1, 2024
Endogenous
substances,
which
are
produced
by
the
body
itself,
have
become
a
focus
of
concern
due
to
their
potential
abuse
as
stimulants
in
sports.
The
chemical
structure
and
physicochemical
properties
externally
synthesized
endogenous
substances
similar
those
internally,
making
them
difficult
detect.
In
response
this
challenge,
it
is
crucial
develop
effective
methods
for
detecting
both
exogenous
substances.
One
promising
approach
leverages
differences
stable
carbon
isotope
abundance
between
internal
external
sources,
leading
changes
Compound-Specific
Isotope
Ratio
(CIR)
after
ingestion.
GC-C-IRMS
method
has
emerged
commonly
used
highly
accurate
technique
tracing
origin
Recognizing
its
significance
fight
against
doping,
World
Anti-Doping
Agency
(WADA)
standardized
established
relevant
benchmarks.
This
review
article
examines
advancements
made
over
past
decade
S1
anabolic
steroids,
S4
hormones
metabolic
modulators,
S9
glucocorticoids.
By
offering
comprehensive
overview
progress
detection
using
ratio
mass
spectrometry,
aim
provide
valuable
insights
enhancing
existing
anti-doping
measures.
research
serves
critical
reference
point
ongoing
efforts
combat
misuse
realm
Frontiers in Public Health,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 27, 2024
This
study
aimed
to
systematically
review
published
randomized
controlled
trials
on
the
effects
of
physical
exercise
activities
daily
living
(ADL)
in
Alzheimer's
patients
through
meta-analysis,
thereby
synthesizing
existing
evidence
provide
scientific
intervention
recommendations
for
prescriptions
patients.