Identification and verification of ferroptosis-related genes in gastric intestinal metaplasia DOI Creative Commons
Biao Song, Tingting Li, Yi Zhang

и другие.

Frontiers in Genetics, Год журнала: 2023, Номер 14

Опубликована: Апрель 18, 2023

Background: Gastric intestinal metaplasia (IM) is the key link of gastric precancerous lesions. Ferroptosis a novel form programmed cell death. However, its impact on IM unclear. The focus this study to identify and verify ferroptosis-related genes (FRGs) that may be involved in by bioinformatics analysis. Materials methods: Differentially expressed (DEGs) were obtained from microarray dataset GSE60427 GSE78523 downloaded Gene Expression Omnibus (GEO) database. (DEFRGs) overlapping DEGs FRGs got FerrDb. DAVID database was used for functional enrichment Protein-protein interaction (PPI) analysis Cytoscape software screen hub gene. In addition, we built receiver operating characteristic (ROC) curve verified relative mRNA expression quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Finally, CIBERSORT algorithm analyze immune infiltration IM. Results: First, total 17 DEFRGs identified. Second, gene module identified considered as gene: PTGS2, HMOX1, IFNG, NOS2. Third, ROC showed HMOX1 NOS2 had good diagnostic characteristics. qRT-PCR experiments confirmed differential normal tissues. immunoassay proportion T cells regulatory (Tregs) macrophages M0 relatively higher, while CD4 memory activated dendritic lower. Conclusion: We found significant associations between IM, biomarkers therapeutic targets These results enhance our understanding contribute treatment.

Язык: Английский

Overcoming cancer chemotherapy resistance by the induction of ferroptosis DOI
Yumin Wang,

Xiaorui Wu,

Ren Zhao

и другие.

Drug Resistance Updates, Год журнала: 2022, Номер 66, С. 100916 - 100916

Опубликована: Дек. 29, 2022

Язык: Английский

Процитировано

179
Targeting ferroptosis in gastric cancer: Strategies and opportunities DOI

Jiahan Le,

Guangzhao Pan,

Che Zhang

и другие.

Immunological Reviews, Год журнала: 2023, Номер 321(1), С. 228 - 245

Опубликована: Окт. 30, 2023

Summary Ferroptosis is a novel form of programmed cell death morphologically, genetically, and biochemically distinct from other pathways characterized by the accumulation iron‐dependent lipid peroxides oxidative damage. It now understood that ferroptosis plays an essential role in various biological processes, especially metabolism iron, lipids, amino acids. Gastric cancer (GC) prevalent malignant tumor worldwide with low early diagnosis rates high metastasis rates, accounting for its relatively poor prognosis. Although chemotherapy commonly used to treat GC, drug resistance often leads therapeutic outcomes. In last several years, extensive research on has highlighted significant potential GC therapy, providing promising strategy address associated standard therapies. this review, we offer summary key regulatory factors related mechanisms underlying ferroptosis. Various inducers inhibitors specifically targeting are uncovered. Additionally, explore prospective applications outcomes these agents field emphasizing their capacity improve patient population.

Язык: Английский

Процитировано

24
Ferroptosis-Based Therapeutic Strategies toward Precision Medicine for Cancer DOI

Tianqi Shi,

Xiaofei Chen, Huihui Ti

и другие.

Journal of Medicinal Chemistry, Год журнала: 2024, Номер 67(4), С. 2238 - 2263

Опубликована: Фев. 2, 2024

Ferroptosis is a type of iron-dependent programmed cell death characterized by the dysregulation iron metabolism and accumulation lipid peroxides. This nonapoptotic mode implicated in various physiological pathological processes. Recent findings have underscored its potential as an innovative strategy for cancer treatment, particularly against recalcitrant malignancies that are resistant to conventional therapies. article focuses on ferroptosis-based therapeutic strategies precision covering molecular mechanisms ferroptosis, four major types ferroptosis inducers their inhibitory effects diverse carcinomas, detection fluorescent probes, implementation image-guided therapy. These state-of-the-art tactics manifested enhanced selectivity efficacy malignant carcinomas. Given administration therapy still at burgeoning stage, some challenges future perspectives discussed clinical translation into treatment.

Язык: Английский

Процитировано

14
POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer DOI Creative Commons
Jingyun Wang,

Qiaoyu Jia,

Shuqin Jiang

и другие.

Cell Biology and Toxicology, Год журнала: 2024, Номер 40(1)

Опубликована: Янв. 25, 2024

Abstract Objective This study investigated the effect and mechanism of POU6F1 lncRNA-CASC2 on ferroptosis gastric cancer (GC) cells. Methods GC cells treated with erastin RSL3 were detected for ferroptosis, reactive oxygen species (ROS) level, cell viability. The expression levels POU6F1, lncRNA-CASC2, SOCS2, ferroptosis-related molecules (GPX4 SLC7A11) also measured. regulations among FMR1, SLC7A11 determined. Subcutaneous tumor models established, in which expressions Ki-67, GPX4 by immunohistochemistry. Results patients decreased had lower survival rate. Overexpression or proliferation GSH cells, addition to increasing total iron, Fe2+, MDA, ROS levels. directly binds promoter promote its transcription. LncRNA-CASC2 can target FMR1 increase SOCS2 mRNA stability ubiquitination degradation activate signaling. Knockdown inhibited sensitivity reversed effects overexpression Conclusion Transcription factor expression, while upregulated increases targeting thereby inhibiting signaling inhibit progression.

Язык: Английский

Процитировано

12
Interplay of CD36, autophagy, and lipid metabolism: insights into cancer progression DOI
Yuxuan Yang, Xiaokun Liu, Di Yang

и другие.

Metabolism, Год журнала: 2024, Номер 155, С. 155905 - 155905

Опубликована: Март 26, 2024

Язык: Английский

Процитировано

12
HDAC inhibitors activate lipid peroxidation and ferroptosis in gastric cancer DOI Creative Commons
Robert Jenke, Denys Oliinyk,

Tamara Zenz

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 225, С. 116257 - 116257

Опубликована: Май 4, 2024

Gastric cancer remains among the deadliest neoplasms worldwide, with limited therapeutic options. Since efficacies of targeted therapies are unsatisfactory, drugs broader mechanisms action rather than a single oncogene inhibition needed. Preclinical studies have identified histone deacetylases (HDAC) as potential targets in gastric cancer. However, mechanism(s) HDAC inhibitors (HDACi) only partially understood. This is particularly true regard to ferroptosis an emerging concept cell death. In panel lines different molecular characteristics, tumor inhibitory effects HDACi were studied. Lipid peroxidation levels measured and proteome analysis was performed for in-depth characterization alterations upon inhibition. on important genes validated mRNA protein level. Upon treatment, lipid found increased all lines. Class I (VK1, entinostat) showed same toxicity profile pan-HDACi vorinostat. Proteome revealed significant concordant expression proteins related induction. Key enzymes like ACSL4, POR or SLC7A11 distinct their patterns, providing explanation peroxidation. Results also confirmed primary human tissue cultures relevant ex vivo model. We identify induction new mechanism class Notably, these findings independent genetic background lines, thus introducing more general principle.

Язык: Английский

Процитировано

10
Circular RNA circBNC2 inhibits tumorigenesis by modulating ferroptosis and acts as a nanotherapeutic target in prostate cancer DOI Creative Commons
Xiang Pan, Kaiwen Chen, Wei Gao

и другие.

Molecular Cancer, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 24, 2025

Язык: Английский

Процитировано

2
Mendelian randomization analysis of plasma proteins reveals potential novel tumor markers for gastric cancer DOI Creative Commons

Wenhai Fan,

Zhenjiang Wu, Shenghao Xu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 28, 2025

This study aimed to elucidate the potential causal relationship between 4,907 plasma proteins and risk of gastric cancer using a two-sample Mendelian randomization approach. We utilized genome-wide association (GWAS) data perform analyses, treating as exposure factors outcome. Instrumental variables for were selected based on strongly correlated SNPs identified through processing screening GWAS provided by deCode database. employed set statistical methods centered inverse variance weighting (IVW) analysis estimate odds ratios (ORs) effects these susceptibility. According IVW method, 14 associated with (p < 0.005). Specifically, CHST15 (OR = 0.7553, 95% CI 0.6346 − 0.8988), L1CAM 0.7230, 0.5876 0.8896), FTMT 0.8246, 0.7241 0.9391), PMM2 0.5767, 0.3943 0.8433) negatively GASTRIC CANCER, whereas ABO 1.1868, 1.0638 1.3240), FAM3D 1.2109, 1.0850 1.3515), FAM3B 1.2988, 1.0953 1.5402), ADH7 1.3568, 1.1044 1.6670), MAP1LC3A 1.3704, 1.1194 1.6778), PGLYRP1 1.4071, 1.1235 1.7623), PDE5A 1.7446, 1.2693 2.3978), GLUL 3.1203, 1.5017 6.4839), NFE2L1 3.1759, 1.6163 6.2402), MAFG 3.1945, 1.5329 6.6575) positively correlated. Convergent results from Weighted Median MR-Egger analyses confirmed associations. Reverse indicated that does not significantly alter levels > 0.05). Sensitivity including assessments heterogeneity horizontal pleiotropy, robustness reliability our findings without significant bias. Pathway enrichment gene expression proteins, GO KEGG pathways, revealed CHST15, L1CAM, FTMT, may serve protective against cancer, while ABO, FAM3D, FAM3B, ADH7, MAP1LC3A, PGLYRP1, PDE5A, GLUL, NFE2L1, contribute pathogenesis. These highlight complex biological interactions tumorigenesis, providing valuable insights preventive therapeutic strategies in malignancy management.

Язык: Английский

Процитировано

1
Glycine regulates lipid peroxidation promoting porcine oocyte maturation and early embryonic development DOI
Lepeng Gao, Chang Zhang, Yingying Zheng

и другие.

Journal of Animal Science, Год журнала: 2022, Номер 101

Опубликована: Дек. 27, 2022

Abstract In vitro-cultured oocytes are separated from the follicular micro-environment in vivo and more vulnerable than to changes external environment. This vulnerability disrupts homeostasis of intracellular environment, affecting oocyte meiotic completion, subsequent embryonic developmental competence vitro. Glycine, one main components glutathione (GSH), plays an important role protection porcine However, protective mechanism glycine needs be further clarified. Our results showed that supplementation promoted cumulus cell expansion maturation. Detection development ability significantly increased cleavage rate blastocyst during vitro fertilization (IVF). SMART-seq revealed this effect was related glycine-mediated regulation membrane structure function. Exogenous addition levels anti-oxidant GSH expression anti-oxidant-related genes (glutathione peroxidase 4 [GPX4], catalase [CAT], superoxide dismutase 1 [SOD1], 2 [SOD2], mitochondrial solute carrier family 25, member 39 [SLC25A39]), decreased lipid peroxidation caused by reactive oxygen species (ROS) reduced level malondialdehyde (MDA) enhancing functions mitochondria, peroxisomes droplets (LDs) metabolism-related factors (peroxisome proliferator activated receptor coactivator alpha [PGC-1α], peroxisome proliferator-activated γ [PPARγ], sterol regulatory element binding factor [SREBF1], autocrine motility [AMFR], ATP). These effects ferroptosis maintained normal function membrane. suggest maturation later regulating ROS-induced metabolism, thereby protecting against biomembrane damage.

Язык: Английский

Процитировано

29
Reactive Oxygen Species and H. pylori Infection: A Comprehensive Review of Their Roles in Gastric Cancer Development DOI Creative Commons
Dhiraj Kumar Sah, Archana Arjunan, Bora Lee

и другие.

Antioxidants, Год журнала: 2023, Номер 12(9), С. 1712 - 1712

Опубликована: Сен. 2, 2023

Gastric cancer (GC) is the fifth most common worldwide and makes up a significant component of global burden. Helicobacter pylori (H. pylori) influential risk factor for GC, with International Agency Research on Cancer classifying it as Class I carcinogen GC. H. has been shown to persist in stomach acid decades, causing damage stomach’s mucosal lining, altering gastric hormone release patterns, potentially function. Epidemiological studies have that eliminating reduces metachronous cancer. Evidence shows various molecular alterations are present precancerous lesions associated an infection. However, although can cause oxidative stress-induced cancer, antioxidants being treatment exact mechanism underlying GC etiology not fully understood. This review provides overview recent research exploring pathophysiology pylori-induced stress antioxidant supplements reduce or even eliminate occurrence.

Язык: Английский

Процитировано

19