Organoids in COVID-19: can we break the glass ceiling? DOI Creative Commons
Chiu Wang Chau, Ryohichi Sugimura

Journal of Leukocyte Biology, Год журнала: 2023, Номер 115(1), С. 85 - 99

Опубликована: Авг. 24, 2023

COVID-19 emerged in September 2020 as a disease caused by the virus SARS-CoV-2. The presented pneumonia at first but later was shown to cause multisystem infections and long-term complications. Many efforts have been put into discovering exact pathogenesis of disease. In this review, we aim discuss an emerging tool modeling, organoids, investigation COVID-19. This review will introduce some methods breakthroughs achieved organoids limitations system.

Язык: Английский

3D Airway Epithelial–Fibroblast Biomimetic Microfluidic Platform to Unravel Engineered Nanoparticle-Induced Acute Stress Responses as Exposome Determinants DOI

Melissa Kao Hui Lee,

Hong Kit Lim, Chengxun Su

и другие.

Environmental Science & Technology, Год журнала: 2023, Номер 57(48), С. 19223 - 19235

Опубликована: Ноя. 7, 2023

Insights into how biological systems respond to high- and low-dose acute environmental stressors are a fundamental aspect of exposome research. However, studying the impact low-level exposure in conventional vitro settings is challenging. This study employed three-dimensional (3D) biomimetic microfluidic lung-on-chip (μLOC) platform RNA-sequencing examine effects two model anthropogenic engineered nanoparticles (NPs): zinc oxide (Nano-ZnO) copier center (Nano-CCP). The airway epithelium exposed these NPs exhibited dose-dependent increases cytotoxicity barrier dysregulation (dominance external exposome). Interestingly, even nontoxic (10 μg/mL) compartment Nano-ZnO triggered chemotaxis lung fibroblasts toward epithelium. An increase α smooth muscle actin (α-SMA) expression contractile activity was also observed cells, indicating bystander-like adaptive response internal Further bioinformatics network analysis showed that significantly induced robust transcriptomic upregulated several hub genes associated with development fibrosis. We propose Nano-ZnO, at no observable effect level (NOEL) dose according standards, can function as potent nanostressor disrupt homeostasis. leads cascade profibrotic events cross-tissue fashion. Our findings offer new insights early respiratory harm exposure, paving way for better exposomic understanding this emerging class nanopollutants.

Язык: Английский

Процитировано

4

Review of in-vitro studies evaluating respiratory toxicity of aerosols: impact of cell types, chemical composition, and atmospheric processing DOI
Sudheer Salana, Vishal Verma

Environmental Science Processes & Impacts, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

In recent decades, several cell-based and acellular methods have been developed to evaluate ambient particulate matter (PM) toxicity. Although provide a more comprehensive assessment of PM toxicity, their results are difficult comprehend due the diversity in cellular endpoints, cell types, assays interference chemical components with some assays' techniques. this review, we attempt clarify these issues. We first discuss morphological immunological differences among various macrophage epithelial cells, belonging respiratory systems human murine species, used

Язык: Английский

Процитировано

1

Advanced approaches to overcome biological barriers in respiratory and systemic routes of administration for enhanced nucleic acid delivery to the lung DOI
Gijung Kwak, Daiheon Lee, Jung Soo Suk

и другие.

Expert Opinion on Drug Delivery, Год журнала: 2023, Номер 20(11), С. 1531 - 1552

Опубликована: Ноя. 2, 2023

Introduction Numerous delivery strategies, primarily novel nucleic acid carriers, have been developed and explored to enable therapeutically relevant lung gene therapy. However, its clinical translation is yet be achieved despite over 30 years of efforts, which attributed the inability overcome a series biological barriers that hamper efficient transfer target cells in lung.

Язык: Английский

Процитировано

2

Activation of TLRs by Opportunistic Fungi in Lung Organoids DOI
Veronika Bosáková, Jan Frič, Teresa Zelante

и другие.

Methods in molecular biology, Год журнала: 2023, Номер unknown, С. 271 - 284

Опубликована: Янв. 1, 2023

Язык: Английский

Процитировано

1

Organoids in COVID-19: can we break the glass ceiling? DOI Creative Commons
Chiu Wang Chau, Ryohichi Sugimura

Journal of Leukocyte Biology, Год журнала: 2023, Номер 115(1), С. 85 - 99

Опубликована: Авг. 24, 2023

COVID-19 emerged in September 2020 as a disease caused by the virus SARS-CoV-2. The presented pneumonia at first but later was shown to cause multisystem infections and long-term complications. Many efforts have been put into discovering exact pathogenesis of disease. In this review, we aim discuss an emerging tool modeling, organoids, investigation COVID-19. This review will introduce some methods breakthroughs achieved organoids limitations system.

Язык: Английский

Процитировано

1