Cardiometry,
Год журнала:
2022,
Номер
24, С. 134 - 144
Опубликована: Ноя. 30, 2022
The
aim
hereof
is
to
develop
a
method
for
revealing
the
properties
of
mitochondria
isolated
from
melanoma
skin
form
tumor
nodules
in
body
mice
parenteral
transplantation
mitochondria.
Materials
and
methods.
We
used
experimental
animals
according
following
design:
intraperitoneal
with
B16/F10
Balb/c
Nude
male
mice,
n
=
8.
All
underwent
one-time
based
on
5.2
mg
protein
per
1
animal
0.4
ml
saline
solution.
were
as
references,
which
transplanted
solution
once
into
muscle
intraperitoneally.
Morphological
examination
sections
internal
organs
foci
was
carried
out
after
specimen
paraffin
embedding
hematoxylin-eosin
staining
microscopy
specimens
Axiovert
(Carl
44
Zeiss,
Germany)
Axiovision
4
image
visualization
software
Germany).
Results.
Upon
autopsies
100%
2
weeks
experiment
total
damage
by
revealed.
morphological
B16
demonstrated
lesion
visceral
tissue
abdominal
organs.
Conclusion.
Thus,
use
C57BL/6
caused
growth
development
body.
Our
study
revealed
previously
unknown
melanoma,
consisting
their
ability
induce
upon
cell
transplantation.
Current Cancer Drug Targets,
Год журнала:
2023,
Номер
24(3), С. 288 - 307
Опубликована: Июль 13, 2023
This
review
describes
the
comprehensive
portrait
of
tumor
microenvironment
(TME).
Additionally,
we
provided
a
panoramic
perspective
on
transformation
and
functions
diverse
constituents
in
TME,
underlying
mechanisms
drug
resistance,
beginning
with
immune
cells
metabolic
dynamics
within
TME.
Lastly,
summarized
most
auspicious
potential
therapeutic
strategies.
The
development
of
using
mitochondrial
transplantation
in
medical
therapy
are
currently
discussed.
According
to
the
endosymbiotic
theory,
movement
mitochondria
between
different
cells
is
possible.
Therefore,
an
idea
transferring
purified
from
with
a
limited
lifespan
shorten
immortal
cancer
was
generated.
Human
umbilical
vein
endothelial
cell
(HUVEC)
kind
primary
lifespan.
In
present
study,
HUVEC
were
applied
alter
malignance
B16F10
melanoma
cell.
comparison
basic
function
mitochondria,
exhibited
higher
membrane
potential
(MMP),
while
lower
levels
ATP,
DNA
(mt-DNA)
and
protein
(mt-protein)
as
compared
B16F10.
After
transplanted
migration,
invasion,
proteins
involved
stemness
decreased,
however,
proliferation,
such
cyclin
D1
E1
increased.
addition,
mt-DNA
mt-protein
also
reduced.
As
for
dynamics
reduced
expressions
DRP1
LC3
II
revealed
that
transferred
can
attenuate
mitophagy
mice,
inoculated
carrying
resulted
larger
size
tumor,
which
corresponded
E1.
Interestingly,
N-cadherin,
α-SMA
MMP-9
reveled
tumor
decreased
mesenchymal
characteristics.
despite
did
not
inhibit
proliferation
B16F10,
metastatic
characteristic
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 13, 2023
Abstract
Background
Mitochondrial
transfer
has
been
found
to
play
an
important
role
in
various
diseases,
and
many
mitochondrial
biological
behaviors
can
be
regulated
by
HMGB1.
However,
the
of
HCC
its
relationship
with
HMGB1
are
currently
unknown.
Method
Field
emission
scanning
electron
microscope,
immunofluorescence,
cell
flow
cytometry
were
used
detect
between
cells.
Analyze
change
significance
related
molecules
clinical
samples
through
bioinformatics
analysis
tissue
microarrays.
The
specific
mechanism
was
determined
co-immunoprecipitation,
luciferase
reporter
assay
chromatin
immunoprecipitation.
Cell
metastasis
invasion
ability
detected
vitro
migration
analysis,
as
well
vivo
tail
vein
lung
mice.
Result
We
confirmed
mitochondria
cells
tunnel
nanotubes.
from
higher
invasive
lower
could
enhance
latter,
hypoxic
conditions
increased
During
this
process,
RHOT1,
transport
protein,
promoted
Under
hypoxia,
further
expression
RHOT1
increasing
NFYA
NFYC
subunits
NF-Y
complex.
RAC1,
protein
associated
formation
nanotubes,
also
development
HCC.
Besides,
RAC1
aggregation
membrane
under
hypoxia.
In
samples,
patients
high
HMGB1,
or
had
relatively
shorter
overall
survival
period.
Conclusion
conditions,
promotes
nanotube
RAC1.
Cardiometry,
Год журнала:
2022,
Номер
24, С. 134 - 144
Опубликована: Ноя. 30, 2022
The
aim
hereof
is
to
develop
a
method
for
revealing
the
properties
of
mitochondria
isolated
from
melanoma
skin
form
tumor
nodules
in
body
mice
parenteral
transplantation
mitochondria.
Materials
and
methods.
We
used
experimental
animals
according
following
design:
intraperitoneal
with
B16/F10
Balb/c
Nude
male
mice,
n
=
8.
All
underwent
one-time
based
on
5.2
mg
protein
per
1
animal
0.4
ml
saline
solution.
were
as
references,
which
transplanted
solution
once
into
muscle
intraperitoneally.
Morphological
examination
sections
internal
organs
foci
was
carried
out
after
specimen
paraffin
embedding
hematoxylin-eosin
staining
microscopy
specimens
Axiovert
(Carl
44
Zeiss,
Germany)
Axiovision
4
image
visualization
software
Germany).
Results.
Upon
autopsies
100%
2
weeks
experiment
total
damage
by
revealed.
morphological
B16
demonstrated
lesion
visceral
tissue
abdominal
organs.
Conclusion.
Thus,
use
C57BL/6
caused
growth
development
body.
Our
study
revealed
previously
unknown
melanoma,
consisting
their
ability
induce
upon
cell
transplantation.
