Folia Biologica,
Год журнала:
2023,
Номер
69(5-6), С. 194 - 196
Опубликована: Янв. 1, 2023
Cyclin-dependent
kinases
(CDKs)
are
master
regulators
of
proliferation,
and
therefore
they
represent
attractive
targets
for
cancer
therapy.
Development
selective
CDK4/6
inhibitors
including
palbociclib
revolutionized
the
treatment
advanced
HR
+
/HER2
–
breast
cancer.
Inhibition
leads
to
cell
cycle
arrest
in
G0/G1
phase
eventually
a
permanent
exit
called
senescence.
One
main
features
senescence
is
an
increased
size.
For
many
years,
it
was
believed
that
non-dividing
cells
simply
continue
grow
as
result,
become
excessively
large.
There
now
emerging
evidence
size
cause
rather
than
consequence
arrest.
This
review
aims
summarize
recent
advances
our
understanding
induction,
particular
resulting
from
with
inhibitors.
European Journal of Cell Biology,
Год журнала:
2023,
Номер
102(2), С. 151312 - 151312
Опубликована: Март 23, 2023
Cell
size
has
profound
effects
on
biological
function,
influencing
a
wide
range
of
processes,
including
biosynthetic
capacity,
metabolism,
and
nutrient
uptake.
As
result,
is
typically
maintained
within
narrow,
population-specific
through
control
mechanisms,
which
are
an
active
area
study.
While
the
physiological
consequences
cell
relatively
well-characterized,
less
known
about
its
developmental
consequences,
specifically
transitions.
In
this
review,
we
compare
systems
where
linked
to
transitions,
paying
particular
attention
examples
from
plants.
We
conclude
by
proposing
that
can
offer
simple
readout
complex
inputs,
enabling
flexible
decisions
during
plant
development.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 26, 2023
Single
cells
actively
coordinate
growth
and
division
to
regulate
their
size,
yet
how
this
size
homeostasis
at
the
single-cell
level
propagates
over
multiple
generations
impact
clonal
expansion
remains
fundamentally
unexplored.
Classical
Defining
the
cellular
factors
that
drive
growth
rate
and
proteome
composition
is
essential
for
understanding
manipulating
systems.
In
bacteria,
ribosome
concentration
known
to
be
a
constraining
factor
of
cell
rate,
while
gene
usually
assumed
not
limiting.
Here,
using
single-molecule
tracking,
quantitative
single-cell
microscopy,
modeling,
we
show
genome
dilution
in
Escherichia
coli
cells
arrested
DNA
replication
results
decrease
active
RNA
polymerases
ribosomes.
The
resulting
sub-linear
scaling
total
ribosomes
with
size
leads
sub-exponential
growth,
even
within
physiological
sizes.
Cell
scales
proportionally
number
concentration-dependent
manner.
Tandem-mass-tag
mass
spectrometry
experiments
further
reveal
DNA-to-cell-volume
ratio
remodels
independently
environment.
Altogether,
our
findings
indicate
an
important
driver
exponential
global
modulator
E.
.
Comparison
studies
on
eukaryotic
suggests
principles
expression
across
domains
life.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Сен. 8, 2022
Abstract
Cellular
senescence
plays
an
important
role
in
development,
ageing,
and
cancer
biology.
Senescence
is
associated
with
increased
cell
size,
but
how
this
contributes
to
permanent
cycle
exit
poorly
understood.
Using
reversible
G1
arrests
combined
growth
rate
modulation,
we
examined
the
effects
of
excess
size
on
progression
human
cells.
We
show
that
enlarged
cells
paradoxically
have
high
levels
G1/S
regulators
relative
were
maintained
at
physiological
also
induce
p21,
which
restrains
entry
protects
against
division
failure.
Furthermore,
find
bear
propensity
for
DNA
breakage
concomitant
damage
repair
defects
are
established
during
G1.
Based
these
observations,
propose
impaired
pathways
prime
persistent
replication-acquired
damage,
ultimately
leading
catastrophic
failure
exit.
arXiv (Cornell University),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 1, 2023
Cell
growth
and
gene
expression,
essential
elements
of
all
living
systems,
have
long
been
the
focus
biophysical
interrogation.
Advances
in
single-cell
methods
invigorated
theoretical
studies
into
these
processes.
However,
until
recently,
there
was
little
dialog
between
two
areas
study.
Most
models
for
regulation
assumed
activity
to
be
oblivious
progression
cell
cycle
birth
division.
But
are
numerous
ways
which
periodic
character
cellular
observables
can
modulate
expression.
The
molecular
factors
required
transcription
translation
increase
number
during
cycle,
but
also
diluted
due
continuous
volume.
replication
genome
changes
dosage
those
same
players
provides
competing
targets
regulatory
binding.
Finally,
division
reduces
their
again,
so
forth.
Stochasticity
is
inherent
biological
processes,
manifested
fluctuations
synthesis
degradation
new
components
as
well
random
partitioning
molecules
at
each
notion
expression
stationary
thus
hard
justify.
In
this
review,
we
survey
emerging
paradigm
cell-cycle
regulated
with
an
emphasis
on
global
patterns
rather
than
gene-specific
regulation.
We
discuss
recent
experimental
reports
where
were
simultaneously
measured
individual
cells,
providing
first
glimpses
coupling
two.
While
findings,
not
surprisingly,
differ
among
genes
organisms,
several
emerged
that
attempt
reconcile
differences
form
a
unifying
framework
understanding
growing
cells.
Analytica Chimica Acta,
Год журнала:
2024,
Номер
1317, С. 342906 - 342906
Опубликована: Июнь 25, 2024
Recent
biological
studies
have
demonstrated
that
changes
can
occur
in
the
cellular
genome
and
proteome
due
to
variations
cell
volume.
Therefore,
it
is
imperative
take
volume
into
account
when
analyzing
a
target
protein.
This
consideration
becomes
especially
critical
experimental
models
involving
cells
subjected
different
treatments.
Failure
consider
could
obscure
studied
phenomena
or
lead
erroneous
conclusions.
However,
quantitative
imaging
of
proteins
within
by
LA-ICP-MS
limited
lack
methods
provide
protein
concentration
(protein
mass
over
volume)
rather
than
just
individual
cells.
The
combination
metal
tagged
immunoprobe
with
ruthenium
red
(RR)
labelling
enables
simultaneous
analysis
specific
each
analyzed
LA-ICP-(Q)MS.
results
indicate
CYP1B1
exhibits
quasi–normally
distribution
control
ARPE-19
cells,
whereas
AAPH–treated
reveal
presence
two
distinct
groups,
responding
non–responding
an
vitro
induced
oxidative
stress.
membrane
RR
measurement
Ru
offers
higher
precision
compared
manually
delimitation
perimeter
eliminates
risk
biased
information,
which
be
prone
inter–observer
variability.
proposed
procedure
fast
minimizes
errors
area
assignment
possibility
carry
out
faster
data
treatment
approach
if
relative
volumes
are
compared,
advantageous
for
applications.
work
presents
innovative
strategy
directly
study
LA-ICP-MS.
method
employs
as
marker
Au
nanoclusters
(AuNCs)
immunoprobes
label
interest.
Furthermore,
rapid
processing,
allowing
calculation
concentrations
thus
facilitating
comparison
across
large
datasets.
As
proof–of–concept,
was
quantified
under
both
stress
conditions.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 26, 2024
Cells
remodel
their
proteomes
in
response
to
changing
environments
by
coordinating
changes
protein
synthesis
and
degradation.
In
yeast,
such
degradation
involves
both
proteasomal
vacuolar
activity,
with
a
mixture
of
bulk
selective
autophagy
delivering
many
the
substrates.
Although
these
pathways
are
known
be
generally
important
for
remodeling,
relative
contributions
have
not
been
reported
on
proteome-wide
basis.
To
assess
this,
we
developed
method
pulse-label
methylotrophic
yeast
