Frontiers in Physiology, Год журнала: 2024, Номер 15
Опубликована: Апрель 9, 2024
The effects of lithium (Li) isotopes and their impact on biological processes have recently gained increased attention due to the significance Li as a pharmacological agent potential that isotopic in neuroscience contexts may constitute new example quantum biology. Previous studies shown two isotopes, which differ mass nuclear spin, unusual different
Язык: Английский
Cell Calcium, Год журнала: 2025, Номер 128, С. 103013 - 103013
Опубликована: Фев. 27, 2025
Язык: Английский
Процитировано
0
The Journal of Physiology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 2, 2025
Transient receptor potential ankyrin 1 (TRPA1) and melastatin 3 (TRPM3) are transduction channels of sensory neurons that play major roles in peripheral mechanisms somatosensation, including thermosensation, chemosensation nociception. Recent studies suggest both also contribute to central pain processing at the spinal cord level. TRPA1 TRPM3 highly permeable for Ca2+, suggesting they could regulate Ca2+ signalling synapses. However, information about TRPA1- TRPM3-induced dorsal horn (DH) is lacking. Here, we describe a dual-colour technique simultaneously measuring concentration ([Ca2+]i) terminals DH by green (GCaMP3) red (jRGECO1a) indicators, using two-photon imaging isolated mouse with attached roots (DR). DR stimulation elicited [Ca2+]i transients axonal boutons primary afferents cell bodies neurons. The antagonists AMPA NMDA glutamate receptors, CNQX AP5, inhibited neurons, but not boutons. Selective agonists TRPM3, ASP7663 CIM0216, induced complex responses distinct partially overlapping subsets Concomitant elevations were observed which blocked AP5. Patch clamp recordings from showed CIM0216 markedly enhanced excitatory synaptic activity. In summary, our findings on presynaptic transmission DH. KEY POINTS: somatosensation processing. These two receptors This study uses measure changes response electrical chemical an intact, ex vivo roots. evoked synaptically-driven results help us better understand mediated cord, potentially explaining role these
Язык: Английский
Процитировано
0
Contact, Год журнала: 2024, Номер 7
Опубликована: Янв. 1, 2024
Mitochondria-endoplasmic reticulum contacts (MERCs), also called endoplasmic (ER)-mitochondria contact sites (ERMCS), are the membrane domains, where these two organelles exchange lipids, Ca 2+ ions, and reactive oxygen species. This crosstalk is a major determinant of cell metabolism, since it allows ER to control mitochondrial oxidative phosphorylation Krebs cycle, while conversely, mitochondria provide sufficient ATP proteostasis. MERC metabolic signaling under tethers multitude regulatory proteins. Many proteins have recently been discovered give rise rare diseases if their genes mutated. Surprisingly, share important hallmarks cause neurological defects, sometimes paired with, or replaced by skeletal muscle deficiency. Typical symptoms include developmental delay, intellectual disability, facial dysmorphism ophthalmologic defects. Seizures, epilepsy, deafness, ataxia, peripheral neuropathy can occur upon mutation protein. Given that most secondary functions, some protein-based do not fit into this categorization. Typically, however, affected in those dominant functions unrelated roles MERCs tethering regulation. We discussing avenues pharmacologically target genetic leading based on our novel insight defects lead common characteristics diseases. These shared disorders raise hope they may allow for similar treatment options.
Язык: Английский
Процитировано
3
Current Pharmaceutical Design, Год журнала: 2023, Номер 29(43), С. 3428 - 3441
Опубликована: Дек. 1, 2023
Aim:: Alzheimer’s disease (AD) has been identified as a progressive brain disorder associated with memory dysfunction and the accumulation of β-amyloid plaques neurofibrillary tangles τ protein. Mitochondria is crucial in maintaining cell survival, death, calcium regulation, ATP synthesis. Mitochondrial linked overload have involved pathogenesis AD. CRM2 (Collapsin response mediator protein-2) endosomal lysosomal trafficking well autophagy, their reduced level also primary culprit progression In addition, Cholinergic neurotransmission neuroinflammation are two other mechanisms implicated AD onset might be protective targets to attenuate progression. The microbiota-gut-brain axis (MGBA) another target for treatment. Crosstalk between gut microbiota mutually benefitted each other, dysbiosis affects functions leads increased AD-causing biomarkers. Despite complexity AD, treatment only limited symptomatic management. Therefore, there an urgent demand novel therapeutics that pathways responsible pathology. This review explores role different possible therapeutic protect against Background:: Amidst various age-related diseases, most deleterious neurodegenerative more than 24 million people globally. Every year, approximately 7.7 new cases dementia reported. However, date, no disease-modifying therapies available treat Objective:: aim writing this highlight key biomarker proteins interventions could play mitigating ongoing prognosis disease. Materials Methods: information about was collected through multiple search engines, including PubMed, Science Direct, Clinical Trials, Google Scholar. Results:: Accumulated pieces evidence reveal extracellular aggregation intracellular protein peculiar features perpetuated (AD). Further, significant mitochondria, calcium, cholinergic makes respiratory organelle All currently drugs either delay clinical damage cells or temporarily some symptoms Conclusion:: pathological deposition β-amyloid, acetylcholinesterase deregulation, multifactorial heterogeneity demands research work field find biological targets.
Язык: Английский
Процитировано
7
Frontiers in Physiology, Год журнала: 2024, Номер 15
Опубликована: Апрель 9, 2024
The effects of lithium (Li) isotopes and their impact on biological processes have recently gained increased attention due to the significance Li as a pharmacological agent potential that isotopic in neuroscience contexts may constitute new example quantum biology. Previous studies shown two isotopes, which differ mass nuclear spin, unusual different
Язык: Английский
Процитировано
2