Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 11, 2024
Abstract
Background
Utilizing
Raman
spectroscopy
detection
technique
for
assessing
the
clinical
pregnancy
outcomes
following
in
vitro
fertilization-embryo
transfer
(IVF-ET).
Methods
A
total
of
76
couples
took
part
research.
During
their
infertility
treatment,
culture
medium
samples
were
collected
from
embryos
(40
and
36
non-pregnancy)
at
both
D3
D5
stages.
In
total,
747
spectra
recorded
these
samples.
The
statistical
analysis
results
evaluated
using
accuracy,
precision,
recall,
F1-score,
Area
Under
Curve(AUC)
to
assess
classification
performance
fusion
model.
biochemical
involved
comparing
significant
differences
spectral
signal
intensities
embryo
metabolites
corresponding
different
outcomes.
Results
This
study
established
a
model
based
on
data
predicting
potential
embryos.
combination
employing
Hilbert-PCA,
spectrogram
concatenation
fusion,
SVM
achieved
an
accuracy
over
93%.
Furthermore,
indicated
features
obtained
with
varying
potentials.
Conclusions
utilized
non-invasive
technology
analyze
metabolic
across
multiple
developmental
By
establishing
model,
high-precision
prediction
was
achieved.
Trial
registration:
study,
ethical
review
number
2021
−
384,
approved
by
Institutional
Review
Board
Yuhuangding
Hospital,
affiliated
Qingdao
University.
Biology of Reproduction,
Год журнала:
2024,
Номер
110(6), С. 1157 - 1174
Опубликована: Апрель 22, 2024
Abstract
Embryo
quality
is
an
important
determinant
of
successful
implantation
and
a
resultant
live
birth.
Current
clinical
approaches
for
evaluating
embryo
rely
on
subjective
morphology
assessments
or
invasive
biopsy
genetic
testing.
However,
both
can
be
inherently
inaccurate
crucially,
fail
to
improve
the
birth
rate
following
transfer
in
vitro
produced
embryos.
Optical
imaging
offers
potential
non-invasive
accurate
avenue
assessing
viability.
Recent
advances
various
label-free
optical
have
garnered
increased
interest
field
reproductive
biology
due
their
ability
rapidly
capture
images
at
high
resolution,
delivering
morphological
molecular
information.
This
burgeoning
holds
immense
further
development,
with
profound
implications
translation.
Here,
our
review
aims
to:
(1)
describe
principles
systems,
distinguishing
between
that
information,
(2)
highlight
recent
application
these
technologies
biology,
(3)
assess
respective
merits
limitations
concerning
capacity
evaluate
quality.
Additionally,
summarizes
challenges
translation
systems
into
routine
practice,
providing
recommendations
future
development.
Finally,
we
identify
suitable
interrogating
mechanisms
underpinning
Frontiers in Endocrinology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 29, 2024
Purpose
With
the
rapid
advancement
of
time-lapse
culture
and
artificial
intelligence
(AI)
technologies
for
embryo
screening,
pregnancy
rates
in
assisted
reproductive
technology
(ART)
have
significantly
improved.
However,
clinical
fresh
cycles
remain
dependent
on
number
type
embryos
transferred.
The
selection
with
highest
implantation
potential
is
critical
embryologists
influences
transfer
strategies
fertility
centers.
superiority
AI
over
traditional
morphological
scoring
ranking
cleavage-stage
based
their
remains
controversial.
Methods
This
retrospective
study
analyzed
105
at
Centre
Reproductive
Medicine
from
August
2023
to
March
2024,
following
IVF/ICSI
treatment
cleavage
stage.
All
were
cultured
using
scored
an
automated
model
(iDAScore
V2.0).
Embryos
categorized
into
three
groups
iDAScore
V2.0:
Group
A
(8
cells,
iDA:
1.0-5.7);
B
5.8-8.0);
C
(>8
5.8-8.0).
Clinical
outcomes,
embryonic
development,
outcomes
compared
across
groups.
Results
Baseline
characteristics
such
as
patient
age,
AMH
levels,
AFC,
basal
sex
hormones
showed
no
significant
differences
among
(p
>
0.05).
iDAscores
higher
(7.3
±
0.5)
(6.7
(4.8
1.0)
<
0.001).
mean
high-quality
was
(4.7
3.0),
followed
by
(3.6
1.7)
(2.1
1.2)
There
statistical
difference
=
0.392)
ongoing
rate
single
transfers
between
(54.5%,
30/55)
(38.1%,
8/21),
although
there
a
tendency
be
higher.
Conclusion
Combining
may
enhance
cycles.
Journal of Clinical Medicine,
Год журнала:
2025,
Номер
14(1), С. 244 - 244
Опубликована: Янв. 3, 2025
Background:
In
this
research,
we
retrospectively
studied
the
influence
of
IVF
vs.
ICSI
technique
on
embryo
morphokinetics
by
means
a
time-lapse
incubator
in
fresh
cycles.
Methods:
A
total
2645
treatment
cycles
resulting
ovum
pick-up
11,471
fertilized
oocytes
were
included
research
from
2018
to
2022.
The
embryos
grouped
according
or
ICSI.
Embryonic
development
was
monitored
using
incubator,
and
they
transferred
day
3
5.
Results:
group
developed
faster
had
less
fragmentation.
However,
fewer
2PNs
achieved
more
discarded
compared
IVF.
When
sibling
treated
with
either
analyzed,
found
that
resulted
quicker
higher
KIDScores.
Discussion:
anovulation
PCOS
subgroups
primary
contributors
high
KIDScores
oocytes,
indicating
might
have
beneficial
effects
oocyte
factors,
similar
positive
results
it
provides
when
male
factors
are
involved.
Conclusions:
Women
undergoing
better
used
spontaneous
This
study
reveals
is
superior
female
factor
infertility.
Frontiers in Endocrinology,
Год журнала:
2025,
Номер
16
Опубликована: Май 8, 2025
This
review
explores
the
non-invasive
applications
of
Raman
spectroscopy
in
assisted
reproductive
technology
(ART).
spectroscopy,
a
powerful
tool
for
analyzing
biological
samples,
has
shown
great
potential
enhancing
ART
outcomes
through
various
such
as
sperm
quality
assessment,
oocyte
evaluation,
and
embryo
selection.
The
non-destructive
nature
high
specificity
this
technique
enable
detailed
biochemical
analysis
at
cellular
level,
offering
valuable
insights
into
processes
without
harming
samples.
highlights
recent
advancements
studies
demonstrating
efficacy
improving
selection
criteria
gametes
embryos,
ultimately
contributing
to
higher
success
rates
ART.
Future
perspectives
on
integrating
with
other
technologies
further
enhance
its
applicability
medicine
are
also
discussed.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4928 - 4928
Опубликована: Май 21, 2025
There
are
two
types
of
noninvasive
biomarkers
human
embryo
developmental
potential:
those
based
on
a
direct
assessment
morphology
over
time
and
using
spent
media
after
in
vitro
culture
as
source
information.
Both
derived
from
previously
acquired
knowledge
different
aspects
pre-implantation
development.
These
include
kinetics,
chromosomal
ploidy
status,
metabolism,
embryonic
gene
transcription,
translation,
expression.
As
to
the
pertinent
data
can
be
obtained
by
analyzing
sequential
microscopic
images
cultured
embryos.
Spent
serve
genomic,
metabolomic,
transcriptomic
proteomic
markers.
Methods
used
early
pioneering
studies,
such
microscopy,
fluorescence
situ
hybridization,
autoradiography,
electrophoresis
immunoblotting,
or
enzyme-linked
immunosorbent
assay,
too
subjective,
invasive,
and/or
time-consuming.
such,
they
unsuitable
for
current
fertilization
(IVF)
practice,
which
needs
objective,
rapid,
selection
best
uterine
transfer
cryopreservation.
This
has
been
made
possible
use
high-throughput
techniques
time-lapse
(for
evaluation),
next-generation
sequencing,
quantitative
real-time
polymerase
chain
reaction,
high-performance
liquid
chromatography,
nanoparticle
tracking
analysis,
flow
cytometry,
mass
spectroscopy,
Raman
near-infrared
nuclear
magnetic
resonance
spectroscopy
analysis).
In
this
review,
individual
markers
presented
systematically,
with
each
marker’s
history
including
available
methodologies,
strengths,
limitations,
so
make
essential
information
accessible
all
health
professionals,
even
whose
expertise
matter
is
limited.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(9), С. 4977 - 4977
Опубликована: Май 2, 2024
One-carbon
(1-C)
metabolic
deficiency
impairs
homeostasis,
driving
disease
development,
including
infertility.
It
is
of
importance
to
summarize
the
current
evidence
regarding
clinical
utility
1-C
metabolism-related
biomolecules
and
methyl
donors,
namely,
folate,
betaine,
choline,
vitamin
B12,
homocysteine
(Hcy),
zinc,
as
potential
biomarkers,
dietary
supplements,
culture
media
supplements
in
context
medically
assisted
reproduction
(MAR).
A
narrative
review
literature
was
conducted
PubMed/Medline
database.
Diet,
ageing,
endocrine
milieu
individuals
affect
both
metabolism
fertility
status.
In
vitro
fertilization
(IVF)
techniques,
conditions
particular,
have
a
direct
impact
on
activity
gametes
embryos.
Critical
analysis
indicated
that
zinc
supplementation
cryopreservation
may
be
promising
approach
reducing
oxidative
damage,
while
female
serum
levels
employed
possible
biomarker
for
predicting
IVF
outcomes.
Nonetheless,
level
low,
future
studies
are
needed
verify
these
data.
processes,
redox
defense
epigenetic
regulation,
compromised
IVF-derived
The
study
lead
way
towards
improving
MAR
efficiency
safety
ensuring
lifelong
health
infants.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Июль 16, 2024
Objective:
This
study
aimed
to
assess
the
relationship
between
implantation
and
soluble
HLA-G
(sHLA-G)
expression
in
cleavage
embryo
culture
medium
(ECM)
conjunction
with
early
developmental
kinetics
determined
by
time-lapse
imaging
(TLI).
Methods:
A
retrospective,
single-center
was
conducted
involving
238
embryos
from
165
patients
who
underwent
Frozen-thawed
transfer
(FET)
using
autologous
oocytes,
either
single
or
double
transfer.
TLI
morphokinetic
parameters
(t2,
t3,
t4,
t5,
t6,
t7,
t8,
cc2,
s2,
cc3,
s3)
of
were
analyzed,
sHLA-G
levels
D3
ECM
measured
an
enzyme-linked
immunosorbent
assay
(ELISA).
hierarchical
classification
model
developed
categorize
into
five
groups
(A,
B,
C,
D,
E).
The
correlation
levels,
embryos,
investigated
establish
a
non-invasive
method
for
evaluating
potential.
Multivariate
logistic
regression
analysis
performed
identify
potential
influencing
factors,
receiver
operating
characteristic
(ROC)
curves
used
evaluate
predictive
value
implantation.
Results:
unconditional
indicated
that
t5
s3
level
independent
risk
factors
affecting
rate
decreased
E.
proposed
effectively
assessed
embryos.
higher
positive
group
compared
negative
(
p
<
0.001).
ECM,
combined
model,
accurately
evaluated
AUC
0.876.
Conclusion:
integration
embryonic
could
reliably
high
likelihood
successful
