Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 30, 2023
Abstract
Background:
Osteoking
has
been
used
for
fracture
therapy
with
a
satisfying
clinical
efficacy.
However,
its
therapeutic
properties
and
the
underlying
mechanisms
remain
elusive.
Method:
A
bone
defect
rat
model
was
established
to
evaluate
pharmacological
effects
of
by
dynamic
observation
X-ray,
micro-CT
histopathologic
examination.
Transcriptome
profiling
performed
identify
defect-related
genes
effective
targets.
Then,
"disease-related
gene-drug
target"
interaction
network
constructed
list
key
targets
were
screened,
which
experimentally
verified.
Results:
effectively
promoted
repair
in
rats
accelerating
cortical
growth
trabeculae.
Histopathologically,
displayed
lower
scores
bone,
cancellous
connection
than
normal
controls,
exerted
favorable
effect
dose-dependent
manner
(all
P<0.001).
The
abnormal
serum
levels
turnover
markers,
factors
metabolism-related
biochemical
indexes
also
reversed
treatment.
Following
transcriptome-based
investigation,
we
hypothesized
that
osteoking
might
attenuate
ZBP1-STAT1-PKR-MLKL-mediated
necroptosis
involved
into
defect.
Experimentally,
expression
ZBP1,
STAT1,
PKR
hallmark
inflammatory
cytokines
end
distinctly
elevated
rats,
but
all
treatment,
suppressed
activities
RIPK1,
RIPK3
MLKL
tissue
supernatants.
Conclusions:
may
promote
formation
regulating
ZBP1-STAT1-PKR
axis,
leading
inhibit
RIPK1/RIPK3/MLKL
activation-mediated
necroptosis.
Journal of Personalized Medicine,
Год журнала:
2024,
Номер
14(9), С. 957 - 957
Опубликована: Сен. 9, 2024
Degenerative
scoliosis
(DS),
encompassing
conditions
like
spondylolisthesis
and
spinal
stenosis,
is
a
common
type
of
deformity.
Lumbar
interbody
fusion
(LIF)
stands
as
conventional
surgical
intervention
for
this
ailment,
aiming
at
decompression,
restoration
intervertebral
height,
stabilization
motion
segments.
Despite
its
widespread
use,
the
precise
mechanism
underlying
remains
elusive.
In
review,
our
focus
lies
on
endochondral
ossification
fusion,
process
involving
vertebral
development
bone
healing.
Endochondral
key
step
successful
fusion.
can
persist
in
hypoxic
promote
parallel
angiogenesis
osteogenesis,
which
corresponds
to
new
formation
region
between
vertebrae.
The
ideal
material
cages
should
have
following
characteristics:
(1)
Good
biocompatibility;
(2)
Stable
chemical
properties;
(3)
Biomechanical
properties
similar
tissue;
(4)
Promotion
fusion;
(5)
Favorable
imaging
observation;
(6)
Biodegradability.
Utilizing
cartilage-derived
bone-like
constructs
holds
promise
promoting
bony
post-operation,
thus
warranting
exploration
context
procedures.
Eastern Ukrainian Medical Journal,
Год журнала:
2024,
Номер
12(3), С. 476 - 491
Опубликована: Янв. 1, 2024
Introduction.
Reparative
osteogenesis
is
a
staged
mechanism
that
ensures
the
restoration
of
damaged
bone
tissue.
The
study
and
summarization
current
data
about
defect
regeneration
basis
for
search
development
methods
to
improve
this
process.
aim
analyze
systematize
reparative
osteogenesis,
describe
main
stages
their
patterns.
Materials
methods.
Searching
relevant
sources
using
online
scientific
databases
keywords.
Selection
publications
review.
Further
analysis
synthesis
information.
results.
Despite
different
interpretations
by
researchers,
they
all
same
coordinated
following
key
have
been
identified:
haematoma
formation,
inflammation,
MSC
involvement
angiogenesis,
cartilage,
connective
tissue
subsequent
reorganisation,
ossification
remodelling.
Each
these
involves
specific
cellular
elements,
local
general
regulatory
factors.
osteogenic
cells
mechanisms
intercellular
interaction,
as
well
signalling
pathways
molecules
(Wnt,
RANK/RANKL/OPG,
BMPs,
HIF,
etc.)
regulate
osteo-
angiogenesis
are
described.
role
hypoxia
in
process
highlighted.
H-type
vessels
participation
regulation
considered.
Attention
paid
phenomenon
‘chondrocyte
transdifferentiation’,
which
one
osteoblasts
during
endochondral
ossification.
In
context
remodelling,
law
biomechanics
piezoelectric
adaptive
multicellular
units
microsystems
ensure
restructuring
coarse
fibrous
into
lamellar
tissue,
Conclusions.
complicated
at
both
molecular
levels.
depends
on
numerous
systemic
factors
optimization
carried
out
each
stage
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 30, 2023
Abstract
Background:
Osteoking
has
been
used
for
fracture
therapy
with
a
satisfying
clinical
efficacy.
However,
its
therapeutic
properties
and
the
underlying
mechanisms
remain
elusive.
Method:
A
bone
defect
rat
model
was
established
to
evaluate
pharmacological
effects
of
by
dynamic
observation
X-ray,
micro-CT
histopathologic
examination.
Transcriptome
profiling
performed
identify
defect-related
genes
effective
targets.
Then,
"disease-related
gene-drug
target"
interaction
network
constructed
list
key
targets
were
screened,
which
experimentally
verified.
Results:
effectively
promoted
repair
in
rats
accelerating
cortical
growth
trabeculae.
Histopathologically,
displayed
lower
scores
bone,
cancellous
connection
than
normal
controls,
exerted
favorable
effect
dose-dependent
manner
(all
P<0.001).
The
abnormal
serum
levels
turnover
markers,
factors
metabolism-related
biochemical
indexes
also
reversed
treatment.
Following
transcriptome-based
investigation,
we
hypothesized
that
osteoking
might
attenuate
ZBP1-STAT1-PKR-MLKL-mediated
necroptosis
involved
into
defect.
Experimentally,
expression
ZBP1,
STAT1,
PKR
hallmark
inflammatory
cytokines
end
distinctly
elevated
rats,
but
all
treatment,
suppressed
activities
RIPK1,
RIPK3
MLKL
tissue
supernatants.
Conclusions:
may
promote
formation
regulating
ZBP1-STAT1-PKR
axis,
leading
inhibit
RIPK1/RIPK3/MLKL
activation-mediated
necroptosis.
