Shenge powder inhibits myocardial fibrosis in rats with post-myocardial infarction heart failure through LOXL2/TGF-β1/IL-11 signaling pathway DOI Creative Commons
Hang Xie,

Boyong Qiu,

Haitao Li

и другие.

Journal of Zhejiang University (Medical Sciences), Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

To investigate the effect of Shenge powder (SGP) on myocardial fibrosis in rats with heart failure after infarction and its relation lysyl oxidase like protein 2 (LOXL2)/transforming growth factor-β1 (TGF-β1)/IL-11 signaling pathway. Seventy-two SPF male SD were divided into blank control group, model SGP small dose large positive dose+LOXL2 activator 12 each group. Except for post-myocardial was induced by coronary constriction all groups. Corresponding treatments given immediately successful modeling, once a day 4 weeks. Left ventricular fractional shortening (LVFS) left ejection fraction (LVEF) detected Color Doppler ultrasound imaging. Levels IL-1β IL-6 serum analyzed ELISA method, Myocardial collagen volume (CVF) evaluated Masson staining. Expressions Ⅰ α-smooth muscle actin (α-SMA) tissue immunohistochemical The mRNA expressions matrix metalloproteinase-9 (MMP-9) inhibitor metalloproteinase 1 (TIMP-1) qRT-PCR. Expression LOXL2, TGF-β1, IL-11 proteins Western blotting. Compared LVFS LVEF group decreased, levels elevated, CVF value, α-SMA tissue, MMP-9 TIMP-1 mRNA, increased (all P<0.05). increased, Collagen decreased (P<0.05); while LOXL2 reversed improvement high-dose infarction. may inhibit inhibiting LOXL2/TGF-β1/IL-11

Язык: Английский

Tanshinone IIA Ameliorates Myocardial Ischemia–Reperfusion Injury via Activating HDAC1‐Repressed Nrf2‐xCT/Gpx4/HO‐1 Axis DOI

Ke Yan,

Shenghui Yu, Xiang Gao

и другие.

Chemical Biology & Drug Design, Год журнала: 2025, Номер 105(4)

Опубликована: Апрель 1, 2025

Myocardial ischemia/reperfusion injury (MIRI) brings secondary to heart tissues and involves complicated pathophysiological activities, such as cell death, oxidative stress, inflammation. HDAC1 (Histone Deacetylase 1) has been documented strengthen MIRI; our study intended investigate the concrete regulatory mechanisms functions of tanshinone IIA on in MIRI, which might provide experimental proofs for adjuvant application treatment MIRI. Genecards SwissTargetPrediction websites were utilized download myocardial infarction-related IIA-targeted genes respectively, then String website was applied display protein-protein interaction (PPI) network. The Cytoscape software subsequently used select PPI network hub genes. AutoDockTools PyMOL operate molecular docking visualize results between HDAC1, Oxygen-glucose deprivation/reoxygenation (OGD/R)-treated myocardiocytes model protein levels nuclear factor erythroid 2-related 2 (Nrf2)-regulated pathway examined by western blot, viability apoptosis evaluated CCK8, Tunnel, flow cytometry assays. lactate dehydrogenase, creatine kinase-MB, malondialdehyde, reduced glutathione, Fe2+ assessed corresponding kit, MIRI rat models constructed verify therapeutic effects vivo. Finally, hematoxylin eosin staining immunohistochemistry pathological changes 4-hydroxynonenal respectively. possible target involved infarction process. Tanshinone could bind amino acid residues with high affinity. Besides, elevated OGD/R-treated myocardiocytes, pretreatment ameliorated myocardiocyte apoptosis, release inflammatory mediators, ferroptosis under following OGD/R treatment, abolished upregulation. suppressed expression further activated Nrf2-xCT/Gpx4/HO-1 axis operation. Functionally, pre-injection infarcted areas via activating HDAC1-suppressed attenuate response, HDAC1-repressed axis, promoted salvage

Язык: Английский

Процитировано

0

Calycosin treats acute myocardial infarction via NLRP3 inflammasome: Bioinformatics, network pharmacology and experimental validation DOI
Huajing Yuan,

Quancheng Han,

Hui Yu

и другие.

European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177621 - 177621

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

The Rough Morphotype of Mycobacterium abscessus Enhances Its Virulence Through ROS/p65/NLRP3/GSDMD‐Mediated Macrophage Pyroptosis DOI
Jingren Li, Juan Li, Anqi Li

и другие.

Clinical and Experimental Pharmacology and Physiology, Год журнала: 2025, Номер 52(6)

Опубликована: Апрель 18, 2025

ABSTRACT The rough morphotype of Mycobacterium abscessus exhibits significantly higher virulence compared to the smooth morphotype, yet underlying molecular mechanisms remain incompletely understood. Pyroptosis in macrophages plays a pivotal role lung tissue damage; however, its specific involvement infection remains be fully clarified. In this study, we identified that upregulates ROS/p65/NLRP3/GSDMD signalling pathway, thereby mediating pyroptosis THP‐1‐derived macrophages. This heightened ability induce macrophage is attributed bacterium's capacity sustain intracellular viability and proliferation. These findings offer valuable insights into provide foundation for future therapeutic interventions.

Язык: Английский

Процитировано

0

Tanshinone IIA reduces tubulointerstitial fibrosis by suppressing GSDMD-mediated pyroptosis DOI Creative Commons
Xueling Yang,

Qinglin Luo,

Zhifen Wu

и другие.

Pharmaceutical Biology, Год журнала: 2025, Номер 63(1), С. 364 - 373

Опубликована: Май 7, 2025

Tanshinone IIA (Tan IIA), a bioactive compound derived from the traditional Chinese herb Salvia miltiorrhiza (Family Lamiaceae, Authority Bunge), is well-known for its protective effects in various kidney diseases. However, role obstructive nephropathy has not been thoroughly investigated. This study aimed to explore of Tan mouse model unilateral ureteral obstruction (UUO) and elucidate cellular molecular mechanisms underlying these effects. Gasdermin D (GSDMD) knockout mice their wild-type (WT) littermates underwent UUO surgery, with treatment administered 24 h prior. Human proximal tubular cells (HK-2 cells) were treated TGF-β1 induce fibrosis (50 ng/mL h), followed by (5 μM) an additional 3 h. significantly reduced expression extracellular matrix (ECM) components, including collagen I, α-smooth muscle actin (α-SMA), vimentin fibronectin, mice. attenuated GSDMD-mediated pyroptosis. GSDMD subjected UUO, on ECM gene deposition interstitium reduced. In vitro studies showed that activation fibronectin protein HK-2 cells. may mitigate pyroptosis renal epithelial (RTECs) reduce fibrosis, highlighting potential as therapeutic strategy prevent progression disease after obstruction.

Язык: Английский

Процитировано

0

Shenge powder inhibits myocardial fibrosis in rats with post-myocardial infarction heart failure through LOXL2/TGF-&beta;1/IL-11 signaling pathway DOI Creative Commons
Hang Xie,

Boyong Qiu,

Haitao Li

и другие.

Journal of Zhejiang University (Medical Sciences), Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

To investigate the effect of Shenge powder (SGP) on myocardial fibrosis in rats with heart failure after infarction and its relation lysyl oxidase like protein 2 (LOXL2)/transforming growth factor-β1 (TGF-β1)/IL-11 signaling pathway. Seventy-two SPF male SD were divided into blank control group, model SGP small dose large positive dose+LOXL2 activator 12 each group. Except for post-myocardial was induced by coronary constriction all groups. Corresponding treatments given immediately successful modeling, once a day 4 weeks. Left ventricular fractional shortening (LVFS) left ejection fraction (LVEF) detected Color Doppler ultrasound imaging. Levels IL-1β IL-6 serum analyzed ELISA method, Myocardial collagen volume (CVF) evaluated Masson staining. Expressions Ⅰ α-smooth muscle actin (α-SMA) tissue immunohistochemical The mRNA expressions matrix metalloproteinase-9 (MMP-9) inhibitor metalloproteinase 1 (TIMP-1) qRT-PCR. Expression LOXL2, TGF-β1, IL-11 proteins Western blotting. Compared LVFS LVEF group decreased, levels elevated, CVF value, α-SMA tissue, MMP-9 TIMP-1 mRNA, increased (all P<0.05). increased, Collagen decreased (P<0.05); while LOXL2 reversed improvement high-dose infarction. may inhibit inhibiting LOXL2/TGF-β1/IL-11

Язык: Английский

Процитировано

0