Journal of Zhejiang University (Medical Sciences),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
To
investigate
the
effect
of
Shenge
powder
(SGP)
on
myocardial
fibrosis
in
rats
with
heart
failure
after
infarction
and
its
relation
lysyl
oxidase
like
protein
2
(LOXL2)/transforming
growth
factor-β1
(TGF-β1)/IL-11
signaling
pathway.
Seventy-two
SPF
male
SD
were
divided
into
blank
control
group,
model
SGP
small
dose
large
positive
dose+LOXL2
activator
12
each
group.
Except
for
post-myocardial
was
induced
by
coronary
constriction
all
groups.
Corresponding
treatments
given
immediately
successful
modeling,
once
a
day
4
weeks.
Left
ventricular
fractional
shortening
(LVFS)
left
ejection
fraction
(LVEF)
detected
Color
Doppler
ultrasound
imaging.
Levels
IL-1β
IL-6
serum
analyzed
ELISA
method,
Myocardial
collagen
volume
(CVF)
evaluated
Masson
staining.
Expressions
Ⅰ
α-smooth
muscle
actin
(α-SMA)
tissue
immunohistochemical
The
mRNA
expressions
matrix
metalloproteinase-9
(MMP-9)
inhibitor
metalloproteinase
1
(TIMP-1)
qRT-PCR.
Expression
LOXL2,
TGF-β1,
IL-11
proteins
Western
blotting.
Compared
LVFS
LVEF
group
decreased,
levels
elevated,
CVF
value,
α-SMA
tissue,
MMP-9
TIMP-1
mRNA,
increased
(all
P<0.05).
increased,
Collagen
decreased
(P<0.05);
while
LOXL2
reversed
improvement
high-dose
infarction.
may
inhibit
inhibiting
LOXL2/TGF-β1/IL-11
Clinical and Experimental Pharmacology and Physiology,
Год журнала:
2025,
Номер
52(6)
Опубликована: Апрель 18, 2025
ABSTRACT
The
rough
morphotype
of
Mycobacterium
abscessus
exhibits
significantly
higher
virulence
compared
to
the
smooth
morphotype,
yet
underlying
molecular
mechanisms
remain
incompletely
understood.
Pyroptosis
in
macrophages
plays
a
pivotal
role
lung
tissue
damage;
however,
its
specific
involvement
infection
remains
be
fully
clarified.
In
this
study,
we
identified
that
upregulates
ROS/p65/NLRP3/GSDMD
signalling
pathway,
thereby
mediating
pyroptosis
THP‐1‐derived
macrophages.
This
heightened
ability
induce
macrophage
is
attributed
bacterium's
capacity
sustain
intracellular
viability
and
proliferation.
These
findings
offer
valuable
insights
into
provide
foundation
for
future
therapeutic
interventions.
Pharmaceutical Biology,
Год журнала:
2025,
Номер
63(1), С. 364 - 373
Опубликована: Май 7, 2025
Tanshinone
IIA
(Tan
IIA),
a
bioactive
compound
derived
from
the
traditional
Chinese
herb
Salvia
miltiorrhiza
(Family
Lamiaceae,
Authority
Bunge),
is
well-known
for
its
protective
effects
in
various
kidney
diseases.
However,
role
obstructive
nephropathy
has
not
been
thoroughly
investigated.
This
study
aimed
to
explore
of
Tan
mouse
model
unilateral
ureteral
obstruction
(UUO)
and
elucidate
cellular
molecular
mechanisms
underlying
these
effects.
Gasdermin
D
(GSDMD)
knockout
mice
their
wild-type
(WT)
littermates
underwent
UUO
surgery,
with
treatment
administered
24
h
prior.
Human
proximal
tubular
cells
(HK-2
cells)
were
treated
TGF-β1
induce
fibrosis
(50
ng/mL
h),
followed
by
(5
μM)
an
additional
3
h.
significantly
reduced
expression
extracellular
matrix
(ECM)
components,
including
collagen
I,
α-smooth
muscle
actin
(α-SMA),
vimentin
fibronectin,
mice.
attenuated
GSDMD-mediated
pyroptosis.
GSDMD
subjected
UUO,
on
ECM
gene
deposition
interstitium
reduced.
In
vitro
studies
showed
that
activation
fibronectin
protein
HK-2
cells.
may
mitigate
pyroptosis
renal
epithelial
(RTECs)
reduce
fibrosis,
highlighting
potential
as
therapeutic
strategy
prevent
progression
disease
after
obstruction.
Journal of Zhejiang University (Medical Sciences),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
To
investigate
the
effect
of
Shenge
powder
(SGP)
on
myocardial
fibrosis
in
rats
with
heart
failure
after
infarction
and
its
relation
lysyl
oxidase
like
protein
2
(LOXL2)/transforming
growth
factor-β1
(TGF-β1)/IL-11
signaling
pathway.
Seventy-two
SPF
male
SD
were
divided
into
blank
control
group,
model
SGP
small
dose
large
positive
dose+LOXL2
activator
12
each
group.
Except
for
post-myocardial
was
induced
by
coronary
constriction
all
groups.
Corresponding
treatments
given
immediately
successful
modeling,
once
a
day
4
weeks.
Left
ventricular
fractional
shortening
(LVFS)
left
ejection
fraction
(LVEF)
detected
Color
Doppler
ultrasound
imaging.
Levels
IL-1β
IL-6
serum
analyzed
ELISA
method,
Myocardial
collagen
volume
(CVF)
evaluated
Masson
staining.
Expressions
Ⅰ
α-smooth
muscle
actin
(α-SMA)
tissue
immunohistochemical
The
mRNA
expressions
matrix
metalloproteinase-9
(MMP-9)
inhibitor
metalloproteinase
1
(TIMP-1)
qRT-PCR.
Expression
LOXL2,
TGF-β1,
IL-11
proteins
Western
blotting.
Compared
LVFS
LVEF
group
decreased,
levels
elevated,
CVF
value,
α-SMA
tissue,
MMP-9
TIMP-1
mRNA,
increased
(all
P<0.05).
increased,
Collagen
decreased
(P<0.05);
while
LOXL2
reversed
improvement
high-dose
infarction.
may
inhibit
inhibiting
LOXL2/TGF-β1/IL-11
