Neuronal expression of E2F4DN restores adult neurogenesis in homozygous 5xFAD mice via TrkB signaling DOI
Anna Lozano-Ureña,

Aina M. Llabrés-Mas,

Morgan Ramón‐Landreau

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract The etiology of Alzheimer’s disease (AD) has been associated with impaired neurogenesis in the adult subventricular zone (SVZ), but molecular mechanism leading to this impairment remains poorly understood. Neuronal dysfunction AD-affected brain might lead reduced production neuron-derived paracrine factors acting through receptors necessary for SVZ (ASN). To test hypothesis, we focused on TrkB receptor, which can transduce signals from neurotrophins BDNF and NT4/5, since is known regulate ASN process its function becomes altered AD. Here show that homozygous 5xFAD (h5xFAD) mice. This prevented by administering an AAV.PHP.eB vector expresses neurons transcription factor E2F4 carrying Thr249Ala/Th251Ala mutation (E2F4DN), a gene therapeutic approach previously demonstrated exert multifactorial effects mouse model use culture media conditioned primary cortical expressing E2F4DN was able recover proliferative differentiative capacity neural stem cells (NSCs) isolated h5xFAD effect blocked inhibiting receptor. Accordingly, activation mimicked E2F4DN-conditioned medium NSCs, finding consistent upregulation NT4/5 expression E2F4DN-transduced neurons. We conclude released E2F4DN-expressing phenotype Therefore, includes recovery signaling NSCs.

Язык: Английский

Epigenetic Regulation of Neural Stem Cells in Developmental and Adult Stages DOI Creative Commons

Shu Kunoh,

Hideyuki Nakashima, Kinichi Nakashima

и другие.

Epigenomes, Год журнала: 2024, Номер 8(2), С. 22 - 22

Опубликована: Июнь 4, 2024

The development of the nervous system is regulated by numerous intracellular molecules and cellular signals that interact temporally spatially with extracellular microenvironment. three major cell types in brain, i.e., neurons two glial cells (astrocytes oligodendrocytes), are generated from common multipotent neural stem (NSCs) throughout life. However, NSCs do not have this multipotentiality beginning. During cortical development, sequentially obtain abilities to differentiate into response combinations spatiotemporally modulated cell-intrinsic epigenetic alterations extrinsic factors. After completion brain a limited population remains adult continues produce (adult neurogenesis), thus contributing learning memory. Many biological aspects neurogenesis changes via behavioral control NSCs. Epigenetic dysregulation has also been implicated pathogenesis various diseases. Here, we present recent advances regulation NSC behavior its disorders.

Язык: Английский

Процитировано

4

The “Jekyll Side” of the S100B Protein: Its Trophic Action in the Diet DOI Open Access
Fabrizio Michetti, Vincenzo Romano Spica

Nutrients, Год журнала: 2025, Номер 17(5), С. 881 - 881

Опубликована: Фев. 28, 2025

The calcium-binding S100B protein is concentrated in glial cells (including enteroglial cells) the nervous system. Its conformation and amino acid composition are significantly conserved different species; this characteristic suggests biological role(s) for protein. activity concentration-dependent: low physiological concentrations exert a neurotrophic effect, while high proinflammatory/toxic role. role of currently attracts scientific community's primary attention, protein's action remains unraveled-yet remarkably interesting. This now topical issue due to recently consolidated notion that natural trophic nutrient available breast milk and/or other aliments, possibly interacting with body districts through its impact on microbiota. These recent data may offer novel clues understanding challenging

Язык: Английский

Процитировано

0

Long Non-Coding RNAs in Stem Cell Regulation and Regenerative Medicine: Stemness, Differentiation, and Therapeutic Innovation DOI Creative Commons

Melika Emarati,

Hossein Azizi, Nima Ghasemi

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Long non-coding RNAs (lncRNAs) have emerged as critical regulators in stem cell biology, influencing cellular functions such pluripotency, differentiation, and self-renewal. Their unique ability to modulate gene expression at multiple levels—epigenetic, transcriptional, post-transcriptional—makes lncRNAs powerful tools for controlling fate. In regenerative medicine, understanding the roles of specific can enhance therapeutic approaches, particularly cell-based tissue repair engineering. By modulating lncRNA activity, researchers potentially direct differentiation toward desired lineages, facilitating development functional tissues clinical applications. This chapter explores how influence states, highlights current research therapies, discusses potential future applications where lncRNA-based interventions could drive advancements engineering medicine.

Язык: Английский

Процитировано

0

Neuronal expression of E2F4DN restores adult neurogenesis in homozygous 5xFAD mice via TrkB signaling DOI
Anna Lozano-Ureña,

Aina M. Llabrés-Mas,

Morgan Ramón‐Landreau

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract The etiology of Alzheimer’s disease (AD) has been associated with impaired neurogenesis in the adult subventricular zone (SVZ), but molecular mechanism leading to this impairment remains poorly understood. Neuronal dysfunction AD-affected brain might lead reduced production neuron-derived paracrine factors acting through receptors necessary for SVZ (ASN). To test hypothesis, we focused on TrkB receptor, which can transduce signals from neurotrophins BDNF and NT4/5, since is known regulate ASN process its function becomes altered AD. Here show that homozygous 5xFAD (h5xFAD) mice. This prevented by administering an AAV.PHP.eB vector expresses neurons transcription factor E2F4 carrying Thr249Ala/Th251Ala mutation (E2F4DN), a gene therapeutic approach previously demonstrated exert multifactorial effects mouse model use culture media conditioned primary cortical expressing E2F4DN was able recover proliferative differentiative capacity neural stem cells (NSCs) isolated h5xFAD effect blocked inhibiting receptor. Accordingly, activation mimicked E2F4DN-conditioned medium NSCs, finding consistent upregulation NT4/5 expression E2F4DN-transduced neurons. We conclude released E2F4DN-expressing phenotype Therefore, includes recovery signaling NSCs.

Язык: Английский

Процитировано

0