Mechanical signatures in cancer metastasis

npj Biological Physics and Mechanics., Год журнала: 2025, Номер 2(1)

Опубликована: Фев. 4, 2025

Abstract The cancer metastatic cascade includes a series of mechanical barrier-crossing events, involving the physical movement cells from their primary location to distant organ. This review describes changes that influence tumour proliferation, progression, and metastasis. We identify potential signatures at every step discuss some latest mechanobiology-based therapeutic interventions highlight importance interdisciplinary approaches in diagnosis treatment.

Язык: Английский

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Biochemical and Biophysical Cues of The Extracellular Matrix Modulates Stem Cell Fate: Progress and Prospect in Extracellular Matrix Mimicking Biomaterials

Biomedical Engineering Advances, Год журнала: 2025, Номер unknown, С. 100143 - 100143

Опубликована: Янв. 1, 2025

Язык: Английский

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A Simple Framework for Agent-Based Modeling with Extracellular Matrix

Bulletin of Mathematical Biology, Год журнала: 2025, Номер 87(3)

Опубликована: Фев. 12, 2025

Abstract Extracellular matrix (ECM) is a key component of the cellular microenvironment and critical in multiple disease developmental processes. Representing ECM cell-ECM interactions challenging multiscale problem as they span molecular-level details to tissue-level dynamics. While several computational frameworks exist for modeling, often focus on very detailed modeling individual fibers or represent only single aspect ECM. Using PhysiCell agent-based platform, we developed framework intermediate detail with ability capture bidirectional interactions. We small region ECM, an element, three variables describing its local microstructure: anisotropy, density, overall fiber orientation. To spatially model use array elements. Cells remodel microstructure turn, impacts motility. demonstrate utility this reusability core interaction through examples invasion, wound healing, basement membrane degradation, leader-follower collective migration. Despite relative simplicity framework, it able broad range interest community. Furthermore, representing are accessible simple programming interfaces. This allows them impact cell behaviors, such proliferation death, without requiring custom code each interaction, particularly PhysiCell’s grammar, enabling rapid diverse cell-matrix biology. make available free open source software package at https://github.com/PhysiCell-Models/collective-invasion .

Язык: Английский

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The next revolution in computational simulations: Harnessing AI and quantum computing in molecular dynamics

Current Opinion in Structural Biology, Год журнала: 2024, Номер 89, С. 102919 - 102919

Опубликована: Сен. 21, 2024

Язык: Английский

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How cells align to structured collagen fibrils: a hybrid cellular Potts and molecular dynamics model with dynamic mechanosensitive focal adhesions

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 12

Опубликована: Янв. 6, 2025

Many mammalian cells, including endothelial cells and fibroblasts, align elongate along the orientation of extracellular matrix (ECM) fibers in a gel when cultured vitro. During cell elongation, clusters focal adhesions (FAs) form near poles elongating cells. FAs are mechanosensitive that grow under mechanical tension exerted by cells' pulling on ECM shrink is released. In this study, we use mathematical modeling to study hypothesis reciprocity between sufficient for directing shape changes orientation. We show preferentially stabilized fibers, where can generate higher than directions perpendicular fibers. present hybrid computational model coupling three approaches: first, cellular Potts (CPM) describes an individual contractile cell; second, molecular dynamics (MD) represent as network cross-linked, deformable fibers; third, set ordinary differential equations (ODEs) cell's FAs, terms balance assembly mechanoresponsive disassembly. The resulting shows suffices stiffness-dependent spreading, local remodeling, ECM-alignment-dependent elongation. These combined effects explain how morphology influenced structure mechanics.

Язык: Английский

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Outside influences: The impact of extracellular matrix mechanics on cell migration

Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Collective Transitions from Orbiting to Matrix Invasion in 3D Multicellular Spheroids

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Coordinated cell rotation along a curved matrix interface can sculpt epithelial tissues into spherical morphologies. Subsequently, radially-oriented invasion of multicellular strands or branches occur by local remodeling the confining matrix. These symmetry-breaking transitions emerge from dynamic reciprocity between cells and matrix, but remain poorly understood. Here, we show that spheroids collectively transition circumferential orbiting to radial via bi-directional interactions with surrounding curvature. Initially, exhibit an ellipsoidal shape become rounded as occurs. However, gradually reorient coordinated towards outward strand due accumulation contractile tractions at discrete sites. Remarkably, initial ellipsoid morphology predicts subsequent 2-4 roughly aligned major axis. We then perturb collective migration using osmotic pressure, showing be arrested reversed. also investigate in "mosaic" spheroids, small fraction "leader" weakened cell-cell adhesions impede still invade Finally, establish minimal self-propelled particle model elucidate how is mediated crosstalk cell-matrix adhesion boundary. Altogether, this work elucidates tissue morphogenesis governed interplay behaviors curvature cell-matrix, relevance for embryonic development tumor progression.

Язык: Английский

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Existence of weak solutions for a volume-filling model of cell invasion into extracellular matrix

Journal of Differential Equations, Год журнала: 2025, Номер 428, С. 721 - 746

Опубликована: Фев. 14, 2025

Язык: Английский

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Bioprinting of transparent and adhesive corneal patches: Integrating photo-crosslinkable dopamine-conjugated silk fibroin and decellularized cornea matrix for sutureless tissue integration and regeneration

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141761 - 141761

Опубликована: Март 1, 2025

Язык: Английский

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A hybrid computational model of cancer spheroid growth with ribose-induced collagen stiffening

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 9, 2025

Metastasis, the leading cause of death in cancer patients, arises when cells disseminate from a primary solid tumour to distant organs. Growth and invasion often involve collective cell migration, which is profoundly influenced by cell-cell interactions extracellular matrix (ECM). The ECM’s biochemical composition mechanical properties, such as stiffness, regulate behaviour migration dynamics. Mathematical modelling serves pivotal tool for studying predicting these complex dynamics, with hybrid discrete-continuous models offering powerful approach combining agent-based representations continuum descriptions surrounding microenvironment. In this study, we investigate impact ECM modulated via ribose-induced collagen cross-linking, on spheroid growth invasion. We employed model implemented PhysiCell simulate successfully replicating three-dimensional vitro experiments. incorporates detailed cell-ECM interactions, remodelling, proliferation. Our simulations align experimental observations two breast lines, non-invasive MCF7 invasive HCC 1954, under varying stiffness conditions. results demonstrate that increased due cross-linking inhibits cells, whereas remain largely unaffected. Furthermore, our show higher degradation not only enables but also facilitates formation multicellular protrusions. Conversely, increasing maximum speed can reach enhances while promoting single-cell This understanding interplay between proliferation, paving way future studies incorporating additional characteristics microenvironmental

Язык: Английский

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