MARCH8 ubiquitinates and degrades CEMIP to induce colorectal cancer cell ferroptosis through inactivating PI3K/AKT pathway

Pathology - Research and Practice, Год журнала: 2025, Номер 269, С. 155909 - 155909

Опубликована: Март 11, 2025

Язык: Английский

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PRDX6 knockdown sensitizes multiple myeloma cells to ferroptosis through inactivation of the PI3K/AKT pathway

Expert Review of Hematology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

This study probes into the impact and mechanisms of PRDX6 on ferroptosis multiple myeloma (MM). expression in MM cells were determined using Western blotting qRT-PCR. treated with pLKO-shPRDX6 vectors expressing shRNA alone or combination Erastin/ferrostatin-1 (ferroptosis activator/inhibitor) 740Y-P (PI3K/AKT pathway activator). Cell viability, apoptosis, Fe2+ concentration, ROS levels examined CCK-8, flow cytometric analysis, iron ion detection, DCFH-DA assay, ELISA. Malondialdehyde (MDA) glutathione (GSH) tested ELISA, SLC7A11 GPX4 measured blotting. was sharply increased (p < 0.01). Silencing sensitized to ferroptosis, as indicated by elevated Fe2+, ROS, MDA but decreased GSH, SLC7A11, 0.05), these trends neutralized treatment. Furthermore, knockdown lowered PI3K/AKT pathway-associated molecules 0.05). may exert pro-ferroptotic effects through inactivation pathway, underlying an appealing therapeutic target for MM.

Язык: Английский

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Cytosolic cytochrome c represses ferroptosis

Cell Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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The m6A methyltransferase METTL3 affects ferroptosis in non-small cell lung cancer by regulating the PTEN/PI3K/AKT pathway

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 18, 2025

Non-small cell lung cancer (NSCLC) poses a major threat to human health, METTL3 has been reported promote numerous tumor development by inhibiting ferroptosis. The aim of the present study was explore mechanism action in NSCLC. UALCAN online platform applied analyze and PTEN expression NSCLC their relationship with stages. NCI-H23 NCI-H1975 cells were transfected sh-METTL3, or oe-METTL3 respectively. Then EdU assay employed assess proliferation transwell ability migrate invade. Apoptosis detected using flow cytometry. In addition, m6A methylation levels, oxidative stress indicators, Fe2+ content determined. Furthermore, GPX4 expression, as well PI3K AKT phosphorylation quantified. Finally, knockdown further sh-PTEN. up-regulated closely related overexpression significantly promoted malignant phenotype cells, increased level mRNA, reduced stress, inhibited occurrence ferroptosis apoptosis, led activation PTEN/PI3K/AKT pathway. Conversely, produced opposite effect. Importantly, knockdown-induced rescued sh-PTEN inhibitor Ferrostatin-1. may inhibit activating pathway, suggesting that METTL3-mediated pathway be promising therapeutic target for

Язык: Английский

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Disulfidptosis-related LncRNAs forecast the prognosis of acute myeloid leukemia

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 20, 2025

Acute myeloid leukemia (AML) is a highly aggressive hematologic malignancy with poor prognosis for patients. Disulfidptosis response-related long non-coding RNAs (DRLs) have been demonstrated to be closely associated cancer development. Therefore, this study aims construct prognostic DRL signature and investigate the immune microenvironment AML. RNA-seq clinical data AML patients were obtained from The Cancer Genome Atlas (TCGA) database. A total of 344 disulfidptosis-associated lncRNAs identified, model consisting seven was constructed validated. Two risk groups, high-risk low-risk, identified. robust capacity predict prognosis, worse overall survival in group. Additionally, differential expression relatively higher samples than control via quantitative polymerase chain reaction(qPCR). Kyoto Encyclopedia Genes Genomes (KEGG) infiltration analysis revealed substantial cells enrichment pathways sensitivity drugs varied according their grade. This identified signature, which can effectively better understand mechanism disulfidptosis provides basis personalized immunotherapy

Язык: Английский

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Celastrol alleviates secondary brain injury following intracerebral haemorrhage by inhibiting neuronal ferroptosis and blocking blood-brain barrier disruption

IBRO Neuroscience Reports, Год журнала: 2024, Номер 17, С. 161 - 176

Опубликована: Авг. 6, 2024

Following recent research advancements, an increasing level of evidence had been published to indicate that celastrol exerted a therapeutic effect on range nervous system diseases. This study therefore aimed investigate the potential involvement ferroptosis and blood-brain barrier disruption in intracerebral haemorrhage.

Язык: Английский

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Crosstalk of methylation and tamoxifen in breast cancer (Review)

Molecular Medicine Reports, Год журнала: 2024, Номер 30(4)

Опубликована: Авг. 9, 2024

Tamoxifen is a widely used anti‑estrogen drug in the endocrine therapy of breast cancer (BC). It blocks estrogen signaling by competitively binding to receptor α (ERα), thereby inhibiting growth BC cells. However, with long‑term application tamoxifen, subset patients have shown resistance which leads low overall survival and progression‑free survival. The molecular mechanism mainly due downregulation ERα expression abnormal activation PI3K/AKT/mTOR pathway. Moreover, targeted gene mediated DNA methylation an important regulatory mode control protein expression. In present review, tamoxifen are briefly introduced, followed focus on effect sensitivity. Finally, clinical for described, including its use as prognostic indicator. it hypothesized that when combination could recover tamoxifen.

Язык: Английский

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Methylome-wide analysis in systemic microbial-induced experimental periodontal disease in mice with different susceptibility

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Июль 16, 2024

Objective The study delved into the epigenetic factors associated with periodontal disease in two lineages of mice, namely C57bl/6 and Balb/c. Its primary objective was to elucidate alterations methylome mice distinct genetic backgrounds following systemic microbial challenge, employing high-throughput DNA methylation analysis as investigative tool. Methods Porphyromonas gingivalis ( Pg )was orally administered induce periodontitis both Balb/c lineage. After euthanasia, genomic from maxilla blood were subjected bisulfite conversion, PCR amplification genome-wide using Ovation RRBS Methyl-Seq System coupled Illumina Infinium Mouse Methylation BeadChip. Results Of particular significance profile observed within -induced group lineage, contrasting control groups Utilizing rigorous filtering criteria, we successfully identified a substantial number differentially methylated regions (DMRs) across various tissues comparison groups, shedding light on prevailing hypermethylation non-induced cohorts hypomethylation induced groups. between samples underscored unique patterns specific jaw tissue. Our comprehensive further unveiled statistically significant disparities, particularly promoter regions, several Conclusion differential mouse lines suggest that contribute variations susceptibility. immune regulation inflammatory response provide potential targets for investigation. These findings emphasize importance considering mechanisms development progression periodontitis.

Язык: Английский

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A nanozyme-based drug delivery system to amplify ferroptosis via MET inhibition and photodynamic therapy

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 156920 - 156920

Опубликована: Окт. 1, 2024

Язык: Английский

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Bioinformatics analysis of ferroptosis in frozen shoulder

BMC Medical Genomics, Год журнала: 2024, Номер 17(1)

Опубликована: Сен. 27, 2024

Frozen shoulder is a common disease that significantly affects the patient's life and work. Ferroptosis new type of programmed cell death, which involved in many diseases. However, there have been no studies reporting relationship between frozen shoulders ferroptosis. This study identified potential molecular markers ferroptosis to provide more effective strategies for treatment shoulders.

Язык: Английский

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