The spatial distribution of intermediate fibroblasts and myeloid-derived cells dictate lymph node metastasis dynamics in oral cancer DOI Creative Commons
Soni Shaikh, Harsh Dhar, M. Moorthy

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 13, 2024

Oral cancer poses a significant health challenge due to limited treatment protocols and therapeutic targets. We aimed investigate the invasive margins of gingivo-buccal oral squamous cell carcinoma (GB-OSCC) tumors in terms localization genes types within at various distances that could lead nodal metastasis. collected tumor tissues from 23 resected GB-OSCC samples for gene expression profiling using digital spatial transcriptomics. monitored differential varying between its microenvironvent (TME), performed deconvulation study immunohistochemistry identify cells regulating TME. found tumor–stromal interface (a distance up 200 µm immune cells) is most active region disease progression GB-OSCC. The differentially expressed apex genes, such as FN1 COL5A1, were located stromal ends margins, together with enrichment extracellular matrix (ECM) an immune-suppressed microenvironment, associated lymph node Intermediate fibroblasts, myocytes, neutrophils enriched ends, while cancer-associated fibroblasts (CAFs) ends. intermediate transformed into CAFs relocated adjacent where they participated FN1-mediated ECM modulation. have generated functional organization identified spatially contribute metastasis progression. Our dataset might now be mined discover suitable molecular targets cancer.

Язык: Английский

Harnessing the tumor microenvironment: targeted cancer therapies through modulation of epithelial-mesenchymal transition DOI Creative Commons
Antonino Glaviano,

Hannah Lau,

Lukas M. Carter

и другие.

Journal of Hematology & Oncology, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 13, 2025

The tumor microenvironment (TME) is integral to cancer progression, impacting metastasis and treatment response. It consists of diverse cell types, extracellular matrix components, signaling molecules that interact promote growth therapeutic resistance. Elucidating the intricate interactions between cells TME crucial in understanding progression challenges. A critical process induced by epithelial-mesenchymal transition (EMT), wherein epithelial acquire mesenchymal traits, which enhance their motility invasiveness progression. By targeting various components TME, novel investigational strategies aim disrupt TME's contribution EMT, thereby improving efficacy, addressing resistance, offering a nuanced approach therapy. This review scrutinizes key players emphasizing avenues therapeutically components. Moreover, article discusses implications for resistance mechanisms highlights current toward modulation along with potential caveats.

Язык: Английский

Процитировано

16
Exploring extracellular RNA as drivers of chemotherapy resistance in cancer DOI

Yumna Khan,

Md Sadique Hussain, Prasanna Srinivasan Ramalingam

и другие.

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Янв. 21, 2025

Язык: Английский

Процитировано

2
Role of Cancer Associated Fibroblast (CAF) derived miRNAs on head and neck malignancies microenvironment: a systematic review DOI Creative Commons

Parsa Golestannejad,

Mohamad-Parsa Monkaresi,

Farahnaz Zhian Zargaran

и другие.

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 1, 2025

MicroRNAs (miRNAs) play a key role in regulating gene expression within the tumor microenvironment, influencing cancer progression and therapy response. Cancer-associated fibroblasts (CAFs) contribute to development by secreting exosomal miRNAs that promote proliferation, invasion, resistance. This systematic review evaluates impact of CAF-derived on head neck malignancies. A search was conducted PubMed, Scopus, WOS, Google Scholar following PRISMA guidelines. Studies focusing cancers were included. Data extraction covered study characteristics, miRNA profiling methods, functional roles, clinical significance. The Scirap tool used for quality assessment. Among 921 identified articles, 21 met inclusion criteria. Findings indicate miR-21-5p, miR-106-5p, miR-196a drive oral squamous cell carcinoma (OSCC), while miR-124 miR-34a-5p act as suppressors. In esophageal (ESCC), miR-21 miR-27a/b chemotherapy resistance, whereas miR-100-5p inhibits lymphangiogenesis. (HNSCC), miR-196b may serve diagnostic biomarkers. Exosomal miR-106a-5p promotes nasopharyngeal (NPC) metastasis, miR-7 resistance (HNC). significantly influence progression, These findings highlight their potential biomarkers therapeutic targets, warranting further research personalized treatment strategies.

Язык: Английский

Процитировано

1
Mechanical Stress in GelMA/Fibrin Scaffolds Promotes Angiogenesis by Influencing Fibroblast-Derived Exosome Secretion DOI Creative Commons
Jiaming Wan,

Ting Du,

Zuohua Liu

и другие.

Materials Today Communications, Год журнала: 2025, Номер unknown, С. 111892 - 111892

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Effect of conditioned medium from miRNA-34a transfected gastric cancer-associated fibroblast on peripheral blood mononuclear cells DOI Creative Commons

Mozhgan Esmaili,

Narjes Jafari, Fatemeh Ahmadzadeh

и другие.

BMC Immunology, Год журнала: 2025, Номер 26(1)

Опубликована: Фев. 25, 2025

Cancer-associated fibroblast (CAF) cells play an important role in gastric malignancy. MiRNA dysregulation has been detected CAF cells, which is related to the tumor progression ability of these cells. Therefore, this study aimed evaluate function miRNA34a carcinoma. We transiently transfected miRNA-34a mimic and examined effect overexpressed miRNA on PD-L1 expression using real-time PCR. Next, we evaluated CAF-conditioned medium (CM) immune response viability cancer cell lines. have shown that significantly reduced (p < 0.05). However, conditioned had no significant response. also found CM suppressed line relative control group (P indicated can reduce proliferation. Additionally, may improve via reduction. Thus, could be a potential therapeutic agent treatment. Not applicable.

Язык: Английский

Процитировано

0
Nano-drug delivery strategies affecting cancer-associated fibroblasts to reduce tumor metastasis DOI Creative Commons

Linghui Zou,

Peng Xian,

Qing Pu

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Spatial and single‐cell transcriptomic analysis reveals fibroblasts dependent immune environment in colorectal cancer DOI

Hang Jia,

Xianglin Liu,

Guimin Wang

и другие.

BioFactors, Год журнала: 2025, Номер 51(2)

Опубликована: Март 1, 2025

Abstract Colorectal cancer (CRC) exhibits a complex tumor microenvironment with significant cellular heterogeneity, particularly involving cancer‐associated fibroblasts that influence behavior and metastasis. This study integrated single‐cell RNA sequencing spatial transcriptomics to dissect fibroblast heterogeneity in CRC. Data processing employed Seurat for quality control, principal component analysis dimensionality reduction, t‐Distributed Stochastic Neighbor Embedding visualization. Differentially expressed genes were identified using DESeq2. Immune infiltration was assessed via Single‐Sample Gene Set Enrichment Analysis, CIBERSORT, xCell algorithms. Prognostic through univariate Cox regression, followed by consensus clustering survival analysis. Metabolic pathways explored scMetabolism. Experimental validation involved CCK8, scratch, Transwell assays evaluate the roles of key BGN CERCAM CRC cell proliferation Machine learning‐driven four fibroblast‐associated (TRIP6, TIMP1, BGN, CERCAM) demonstrating prognostic relevance Consensus based on these biomarkers stratified patients into three distinct molecular subtypes (Clusters A–C). Notably, Cluster C exhibited most unfavorable clinical outcomes coupled marked upregulation all fibroblast‐related genes. Comprehensive immune profiling revealed paradoxical features C: heightened global activation (characterized substantial leukocyte infiltration) coexisted specific immunosuppressive elements, including enrichment pro‐tumorigenic M0 macrophages, depletion anti‐tumor plasma cells, resting memory CD4+ T along coordinated multiple checkpoint molecules. Computational prediction TIDE platform suggested enhanced immunotherapy responsiveness patients. Functional demonstrated knockdown or significantly impaired malignant phenotypes, reducing proliferative capacity, migration potential, invasive ability. Fibroblasts demonstrate within microenvironment, impacting prognosis therapeutic responses. Key emerge as potential immunotherapeutic targets, offering new strategies precision treatment

Язык: Английский

Процитировано

0
Prognostic value and anti-tumor immunity role of TMED9 in pan-cancer: a bioinformatics study DOI Open Access

Haodi Wang,

Yue Wang,

Pengyu Tan

и другие.

Translational Cancer Research, Год журнала: 2024, Номер 13(10), С. 5429 - 5445

Опубликована: Сен. 23, 2024

Transmembrane p24 trafficking protein 9 (

Язык: Английский

Процитировано

1
The spatial distribution of intermediate fibroblasts and myeloid-derived cells dictate lymph node metastasis dynamics in oral cancer DOI Creative Commons
Soni Shaikh, Harsh Dhar, M. Moorthy

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 13, 2024

Oral cancer poses a significant health challenge due to limited treatment protocols and therapeutic targets. We aimed investigate the invasive margins of gingivo-buccal oral squamous cell carcinoma (GB-OSCC) tumors in terms localization genes types within at various distances that could lead nodal metastasis. collected tumor tissues from 23 resected GB-OSCC samples for gene expression profiling using digital spatial transcriptomics. monitored differential varying between its microenvironvent (TME), performed deconvulation study immunohistochemistry identify cells regulating TME. found tumor–stromal interface (a distance up 200 µm immune cells) is most active region disease progression GB-OSCC. The differentially expressed apex genes, such as FN1 COL5A1, were located stromal ends margins, together with enrichment extracellular matrix (ECM) an immune-suppressed microenvironment, associated lymph node Intermediate fibroblasts, myocytes, neutrophils enriched ends, while cancer-associated fibroblasts (CAFs) ends. intermediate transformed into CAFs relocated adjacent where they participated FN1-mediated ECM modulation. have generated functional organization identified spatially contribute metastasis progression. Our dataset might now be mined discover suitable molecular targets cancer.

Язык: Английский

Процитировано

0