COL10A1 Facilitates Prostate Cancer Progression by Interacting With INHBA to Activate the PI3K/AKT Pathway DOI Creative Commons
Kun Jiang, Li‐zhe Xu, Cheng Fan

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(23)

Опубликована: Дек. 1, 2024

ABSTRACT Prostate cancer (PCa) constitutes a highly common and lethal disease that impacts males globally. However, the specific molecular pathways responsible for its development are still unknown. Therefore, revealing regulators contributed to progression of PCa is pivotal developing unique management strategies. Through comprehensive bioinformatics analysis multiple public gene databases, we thoroughly investigated COL10A1 expression level, clinical significance, co‐expressed genes signalling in PCa. INHBA level was assessed specimens using RT‐qPCR, Western blotting immunohistochemistry. A combination experimental techniques, including CCK‐8 assay, colony formation, flow cytometry, Transwell, wound‐healing, immunoprecipitation assays rescue study, utilised examine fundamental COL10A1's action across The significantly elevated PCa, upregulation has been connected with tumour aggressiveness weak predictive outcome subjects. current investigation revealed regulation expression, either by or downregulation, resulted sequential augmentation suppression cell progression, migration invasion. Mechanistically, manifested directly interact facilitate PI3K AKT phosphorylation within cells mouse models. results our study offer new perspectives on tumorigenic role interactions may play important roles progression.

Язык: Английский

Causal effects of triacylglycerol concentration on cervical cancer: evidence from two-step mediated Mendelian randomization. DOI Creative Commons

Cong Xu,

Yonghong Xu,

Yunhui Yang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Май 30, 2024

Abstract Cervical cancer ranks as the fourth most prevalent among women globally, and in recent years, there has been widespread attention on role of lipids tumorigenesis development. This study utilized Mendelian Randomization (MR) to explore causal relationship between immune cell-mediated cervical risk. We have selected lipids, which are closely associated with function cells, identified their genetic instrumental variables. Using large-scale genomic association (GWAS) data, we genetically evaluated levels analyzed correlation risk Preliminary results suggest that triacylglycerol is significantly And elevated an increased cancer. In addition, found regulatory cells such BAFF − R naive mature B cell, IgD+, transitional may indirectly influence development by influencing response. Our research, employing inference analysis randomization, demonstrates significance cell-induced progression. These contribute comprehending origins might steer creation upcoming preventive approaches therapeutic tactics. Additional studies essential confirm these investigate particular biological processes.

Язык: Английский

Процитировано

0
Causal effects of triacylglycerol concentration on cervical cancer: evidence from two-step mediated Mendelian randomization DOI Creative Commons

Cong Xu,

Yonghong Xu,

Yunhui Yang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 5, 2024

Abstract Cervical cancer ranks as the fourth most prevalent among women globally, and in recent years, there has been widespread attention on role of lipids tumorigenesis development. This study utilized Mendelian Randomization (MR) to explore causal relationship between immune cell-mediated cervical risk. We have selected lipids, which are closely associated with function cells, identified their genetic instrumental variables. Using large-scale genomic association (GWAS) data, we genetically evaluated levels analyzed correlation risk Preliminary results suggest that triacylglycerol is significantly And elevated an increased cancer. In addition, found regulatory cells such BAFF − R naive mature B cell, IgD+, transitional may indirectly influence development by influencing response. Our research, employing inference analysis randomization, demonstrates significance cell-induced progression. These contribute comprehending origins might steer creation upcoming preventive approaches therapeutic tactics. Additional studies essential confirm these investigate particular biological processes.

Язык: Английский

Процитировано

0
In vitro modulation the Notch pathway by piperine: A therapeutic strategy for docetaxel‐resistant and non‐resistant prostate cancer DOI

Rui‐tao Wang,

Hao‐en Liu,

Hui‐yuan Sun

и другие.

Chemical Biology & Drug Design, Год журнала: 2024, Номер 103(6)

Опубликована: Июнь 1, 2024

Abstract Docetaxel (DTX) resistance poses a significant challenge in the treatment of prostate cancer (PCa), often leading to chemotherapy failure. This study investigates ability piperine, compound derived from black pepper, enhance sensitivity PCa cells DTX and elucidates its underlying mechanism. We established DTX‐resistant cell line, DU145/DTX, conduct our experiments. Through series assays, including MTT for viability, flow cytometry apoptosis, Transwell migration invasion, western blot protein expression analysis, we assessed effects piperine on these cellular functions Notch signaling pathway components. Our results demonstrated that successfully line DU145/DTX. Piperine effectively decreased viability both DU145 counterpart, concentration time‐dependent manner when used alone combination with DTX. Notably, also induced apoptosis reduced invasion capabilities cells. At molecular level, down‐regulated by inhibiting Notch1 Jagged1 signaling, as well reducing downstream effectors Hey1 hes family bHLH transcription factor 1. The concludes piperine's modulate induce highlights potential complementary PCa, paving way use traditional Chinese medicinal compounds modern oncology strategies.

Язык: Английский

Процитировано

0
Extrachromosomal circular DNAs in prostate adenocarcinoma: global characterizations and a novel prediction model DOI Creative Commons

Qingliu He,

Qingfu Su,

Chengcheng Wei

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Сен. 17, 2024

Background The role of focal amplifications and extrachromosomal circular DNA (eccDNA) is still uncertain in prostate adenocarcinoma (PRAD). Here, we first mapped the global characterizations eccDNA then investigate characterization eccDNA-amplified key differentially expressed encoded genes (eKDEGs) progression, immune response immunotherapy PRAD. Methods Circular_seq was used conjunction with TCGA-PRAD transcriptome dataset to sequence, annotate, filter for coding (eDEGs) PRAD para-cancerous normal tissues. Afterwards, risk models were created eKDEGs linked prognosis identified using Cox Lasso regression analysis. microenvironment model quantified a variety immunological algorithms, which also its characteristics regard immunotherapy, response, infiltration. Results In this research, there no significant difference size, type, chromosomal distribution However, 4,290 eccDNAs 1,981 amplified. Following that, 499 eDEGs tested from TCGA-PRAD. By techniques, ZNF330 PITPNM3 as PRAD, new conducted based on this. Survival analysis showed that high-risk group associated poor validated external data. Immune infiltration risks affected cell not only mediating changes function, but correlating immunophenotyping. Furthermore, negatively anti- CTLA-4 /anti- PD-1 mutational burden. addition, Tumor Dysfunction Exclusion analyses more prone escape. Drug sensitivity 10 drugs, instructive treatment. Conclusion PITPNM are can be potential prognostic markers. two-factor combined effectively assess survival patients, predict different responses may provide ideas immunotherapy.

Язык: Английский

Процитировано

0
CDR1as/miR-7-5p/IGF1R axis contributes to the suppression of cell viability in prostate cancer DOI Creative Commons
Murat Kaya, İlknur Süer, Abdulmelik Aytatli

и другие.

Turkish Journal of Biochemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract Background Prostate cancer is the most frequently diagnosed male and fifth highest cause of mortality in men. CDR1as has played an essential role growth several malignancies. However, its significance progression prostate not been investigated. We aimed to investigate mechanism development identify a new target for diagnostics treatment. Methods siRNA miR-7-5p mimic were transfected into PC3 DU145 PCa cell lines their effects on cellular processes Cell viability was measured by WST-8 assay. The and/or migration detected using scratch-wound apoptotic capacity cells evaluated Caspase-3 kit. potential targets defined via silico tools. mRNA protein expression levels IGF1R EIF4E qRT-PCR western blot assays, respectively. matching between luciferase reporter Results Inhibiting or restoring reduced proliferation while increasing apoptosis. Silencing elevated decreasing IGF1R. Conclusions functions as sponge, promoting tumor development.

Язык: Английский

Процитировано

0
COL10A1 Facilitates Prostate Cancer Progression by Interacting With INHBA to Activate the PI3K/AKT Pathway DOI Creative Commons
Kun Jiang, Li‐zhe Xu, Cheng Fan

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(23)

Опубликована: Дек. 1, 2024

ABSTRACT Prostate cancer (PCa) constitutes a highly common and lethal disease that impacts males globally. However, the specific molecular pathways responsible for its development are still unknown. Therefore, revealing regulators contributed to progression of PCa is pivotal developing unique management strategies. Through comprehensive bioinformatics analysis multiple public gene databases, we thoroughly investigated COL10A1 expression level, clinical significance, co‐expressed genes signalling in PCa. INHBA level was assessed specimens using RT‐qPCR, Western blotting immunohistochemistry. A combination experimental techniques, including CCK‐8 assay, colony formation, flow cytometry, Transwell, wound‐healing, immunoprecipitation assays rescue study, utilised examine fundamental COL10A1's action across The significantly elevated PCa, upregulation has been connected with tumour aggressiveness weak predictive outcome subjects. current investigation revealed regulation expression, either by or downregulation, resulted sequential augmentation suppression cell progression, migration invasion. Mechanistically, manifested directly interact facilitate PI3K AKT phosphorylation within cells mouse models. results our study offer new perspectives on tumorigenic role interactions may play important roles progression.

Язык: Английский

Процитировано

0