Intestinal organoids in inflammatory bowel disease: advances, applications, and future directions DOI Creative Commons
Jianzhen Ren,

Silin Huang

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Май 12, 2025

Inflammatory bowel disease (IBD), characterized by chronic gastrointestinal inflammation, is a significant global health challenge. Traditional models often fail to accurately reflect human pathophysiology, leading suboptimal treatments. This review provides an overview of recent advancements in intestinal organoid technology and its role IBD research. Organoids, derived from patient-specific or pluripotent stem cells, retain the genetic, epigenetic, structural characteristics native gut, allowing for precise modeling key aspects IBD. Innovations CRISPR editing, organoid-microbe co-cultures, organ-on-a-chip systems have enhanced physiological relevance these models, facilitating drug discovery personalized therapy screening. However, challenges such as vascularization deficits need standardized protocols remain. underscores interdisciplinary efforts bridge gap between complex reality Future directions include development scalable vascularized robust regulatory frameworks accelerate therapeutic translation. Organoids hold promise unraveling heterogeneity transforming management.

Язык: Английский

Colorectal Organoids: Models, Imaging, Omics, Therapy, Immunology, and Ethics DOI Creative Commons
Martina Taglieri,

Linda Di Gregorio,

Serena Matis

и другие.

Cells, Год журнала: 2025, Номер 14(6), С. 457 - 457

Опубликована: Март 19, 2025

Colorectal epithelium was the first long-term 3D organoid culture established in vitro. Identification of key components essential for survival stem cell niche allowed an indefinite propagation these cultures and modulation their differentiation into various lineages mature intestinal epithelial cells. While methods were eventually adapted to establish organoids from different organs, colorectal remain a pioneering model development new applications health disease. Several basic applicative aspects culture, modeling, monitoring testing are analyzed this review. We also tackle ethical problems biobanking distribution precious research tools, frequently confined laboratory origin or condemned destruction at end project.

Язык: Английский

Процитировано

0
Biomimetic culture substrates for modelling homeostatic intestinal epithelium in vitro DOI Creative Commons

Sorosh Abdollahi,

Bahareh Zarin,

Maryam Vatani

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 3, 2025

The increasing interest in utilizing three-dimensional (3D) vitro models with innovative biomaterials to engineer functional tissues arises from the limitations of conventional cell culture methods accurately reproducing complex physiological conditions living organisms. This study presents a strategy for replicating intricate microenvironment intestine by cultivating intestinal cells within bioinspired 3D interfaces that recapitulate villus-crypt architecture and tissue arrangement intestine. Intestinal cultured on these biomimetic substrates exhibited phenotypes differentiation characteristics resembling intestinal-specific types, effectively tissue. Notably, proliferation were achieved 72-120 h-significantly faster than several weeks required bioengineered materials, which often pose risks necrosis or cross-contamination. Additionally, differentiated villi-crypts mimicking bio-interfaces exhibit higher production natural antimicrobial peptides, resulting reduced pathogenic infection compared control samples. Furthermore, our method stands out simplicity fabrication, eliminating need cleanroom procedures microfabrication techniques.

Язык: Английский

Процитировано

0
Intestinal organoids in inflammatory bowel disease: advances, applications, and future directions DOI Creative Commons
Jianzhen Ren,

Silin Huang

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Май 12, 2025

Inflammatory bowel disease (IBD), characterized by chronic gastrointestinal inflammation, is a significant global health challenge. Traditional models often fail to accurately reflect human pathophysiology, leading suboptimal treatments. This review provides an overview of recent advancements in intestinal organoid technology and its role IBD research. Organoids, derived from patient-specific or pluripotent stem cells, retain the genetic, epigenetic, structural characteristics native gut, allowing for precise modeling key aspects IBD. Innovations CRISPR editing, organoid-microbe co-cultures, organ-on-a-chip systems have enhanced physiological relevance these models, facilitating drug discovery personalized therapy screening. However, challenges such as vascularization deficits need standardized protocols remain. underscores interdisciplinary efforts bridge gap between complex reality Future directions include development scalable vascularized robust regulatory frameworks accelerate therapeutic translation. Organoids hold promise unraveling heterogeneity transforming management.

Язык: Английский

Процитировано

0