Malaria Journal, Год журнала: 2025, Номер 24(1)

Опубликована: Май 2, 2025

Abstract Background Understanding the molecular mechanisms of malarial parasites in hosts is crucial for developing effective treatments. Epitranscriptomic research on pathogens has unveiled significance RNA methylation gene regulation and pathogenesis. This first report investigating signatures alternative splicing events using Nanopore Direct Sequencing to single-base resolution Plasmodium falciparum vivax clinical isolates with hepatic dysfunction complications. Methods from P. showing manifestation was performed. Subsequently, transcriptome reconstruction FLAIR transcript classification SQANTI3, followed by detection CHEUI m6Anet identify N6-methyladenosine (m6A) 5-methylcytosine (m5C) signatures, done. The both datasets were documented. Results reference genome reports > 5000 genes out which ~ 50% identified as expressed two sequenced isolates, including novel isoforms intergenic transcripts, highlighting extensive diversity. distinct profiles m6A m5C transcripts observed sense, Natural Antisense Transcripts (NATs) categories hinting at species-specific regulatory mechanisms. Dual modification a significant number parasites. Modified originating apicoplast mitochondrial genomes have also been detected. These modifications are unevenly present annotated regions mRNA, potentially influencing mRNA export translation. Several observed, 3′ 5′ end predominating suggesting differences translational kinetics possible protein characteristics these disease conditions. Conclusion data shows presence modified NATs alternatively spliced transcripts. phenomena together suggest multiple layers decides post-translational proteome particular Studies like will help decipher environments malaria vivo elucidate their inherent plasticity, thus allowing conceptualization strategies interventions.

Язык: Английский