Evaluation of the anti-inflammatory, antioxidant and regenerative effects of microbiota-derived postbiotics in human periodontal ligament mesenchymal stromal cells DOI Creative Commons
Hazal Kibar Demirhan,

Emine Omer Oglou,

Zeynep Büşra Aksoy

и другие.

Clinical Oral Investigations, Год журнала: 2025, Номер 29(5)

Опубликована: Апрель 23, 2025

Abstract Objective This study investigates the regenerative and protective effects of postbiotics (cell-free supernatant) derived from Lactiplantibacillus plantarum EIR/IF-1 strain on human periodontal ligament mesenchymal stromal cells (hPDL-MSCs). Materials methods hPDL-MSCs were isolated tissues (PDL) wisdom teeth using enzymatic digestion subsequently characterized through immunophenotyping. The effect viability was assessed MTT assay flow cytometry, while their impact cell migration evaluated via scratch assay. Anti-inflammatory investigated lipopolysaccharide (LPS, Porphyromonas gingivalis )-stimulated Enzyme-Linked Immunosorbent Assay (ELISA). Additionally, antioxidant analyzed in hydrogen peroxide (H₂O₂)-induced by measuring reactive oxygen species (ROS) levels cytometry. expression collagen type I ( COL1A1 ) gene further quantitative reverse transcription PCR immunofluorescence staining. Results Treatment with (250 µg/mL) significantly increased capability hPDL-MSCs, enhancing production for PDL repair. 24 h resulted a 54.53 ± 2.01% reduction intracellular ROS compared to untreated H 2 O -induced hPDL-MSCs. Furthermore, decreased pro-inflammatory cytokines (IL-8, IL-6, IL-1β), anti-inflammatory cytokine IL-10 (2.67-fold) LPS-stimulated Conclusion Our findings indicate that exhibit biological activity throughout all stages healing process, beginning modulation inflammatory response LPS stimulation, followed promotion migration, proliferation, synthesis. Given unmet need safe adjuvant therapeutic approaches promote comprehensive regeneration diseases, this presents as promising candidate. Clinical relevance Postbiotics could be integrated into therapies novel bioactive material improve outcomes defects both controlling inflammation stimulating tissue repair processes.

Язык: Английский

Evaluation of the anti-inflammatory, antioxidant and regenerative effects of microbiota-derived postbiotics in human periodontal ligament mesenchymal stromal cells DOI Creative Commons
Hazal Kibar Demirhan,

Emine Omer Oglou,

Zeynep Büşra Aksoy

и другие.

Clinical Oral Investigations, Год журнала: 2025, Номер 29(5)

Опубликована: Апрель 23, 2025

Abstract Objective This study investigates the regenerative and protective effects of postbiotics (cell-free supernatant) derived from Lactiplantibacillus plantarum EIR/IF-1 strain on human periodontal ligament mesenchymal stromal cells (hPDL-MSCs). Materials methods hPDL-MSCs were isolated tissues (PDL) wisdom teeth using enzymatic digestion subsequently characterized through immunophenotyping. The effect viability was assessed MTT assay flow cytometry, while their impact cell migration evaluated via scratch assay. Anti-inflammatory investigated lipopolysaccharide (LPS, Porphyromonas gingivalis )-stimulated Enzyme-Linked Immunosorbent Assay (ELISA). Additionally, antioxidant analyzed in hydrogen peroxide (H₂O₂)-induced by measuring reactive oxygen species (ROS) levels cytometry. expression collagen type I ( COL1A1 ) gene further quantitative reverse transcription PCR immunofluorescence staining. Results Treatment with (250 µg/mL) significantly increased capability hPDL-MSCs, enhancing production for PDL repair. 24 h resulted a 54.53 ± 2.01% reduction intracellular ROS compared to untreated H 2 O -induced hPDL-MSCs. Furthermore, decreased pro-inflammatory cytokines (IL-8, IL-6, IL-1β), anti-inflammatory cytokine IL-10 (2.67-fold) LPS-stimulated Conclusion Our findings indicate that exhibit biological activity throughout all stages healing process, beginning modulation inflammatory response LPS stimulation, followed promotion migration, proliferation, synthesis. Given unmet need safe adjuvant therapeutic approaches promote comprehensive regeneration diseases, this presents as promising candidate. Clinical relevance Postbiotics could be integrated into therapies novel bioactive material improve outcomes defects both controlling inflammation stimulating tissue repair processes.

Язык: Английский

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