Biomarkers in disease, Год журнала: 2022, Номер unknown, С. 1 - 28
Опубликована: Янв. 1, 2022
Язык: Английский
ACS Omega, Год журнала: 2024, Номер unknown
Опубликована: Март 8, 2024
Leishmaniasis, which is caused by a parasitic protozoan of the genus Leishmania, still major threat to global health, impacting millions individuals worldwide in endemic areas. Chemotherapy has been principal method for managing leishmaniasis; nevertheless, evolution drug resistance offers significant obstacle therapeutic success. Drug-resistant behavior these parasites complex phenomenon including both innate and acquired mechanisms. Resistance frequently related changes transportation, target alterations, enhanced efflux from pathogen. This review revealed specific genetic mutations Leishmania that are associated with commonly used antileishmanial drugs such as pentavalent antimonials, miltefosine, amphotericin B, paromomycin, resulting gene expression along functioning various proteins involved uptake, metabolism, efflux. Understanding linked essential creating approaches tackling avoiding spread drug-resistant variants. Based on treatments focus pathways could potentially improve treatment efficacy help long-term leishmaniasis control. More study needed uncover complete range generating medication develop new therapies based available information.
Язык: Английский
Процитировано
12
Pathogens, Год журнала: 2024, Номер 13(10), С. 835 - 835
Опубликована: Сен. 27, 2024
The protozoan parasite Leishmania causes leishmaniasis, a neglected tropical disease, that disproportionately affects underdeveloped countries. This disease has major health, economic, and social implications, particularly because of the limited treatment options, high cost, severe side effects associated with available therapeutics, rate failure caused by parasites’ growing resistance to current medications. In this review, we describe first common strategies used pathogens develop drug then focus on arsenal drugs treat their modes action, molecular mechanisms contributing in spp., including role genomic, transcriptional, translational control. We more specifically our recent discovery reprogramming as driver leading coordinated changes translation transcripts orchestrating metabolome lipidome support resistance. A thorough understanding these is essential identify key elements needed combat improve leishmaniasis methods.
Язык: Английский
Процитировано
9
Pathogens, Год журнала: 2023, Номер 12(1), С. 70 - 70
Опубликована: Янв. 1, 2023
Leishmaniasis is a neglected disease caused by protozoa belonging to the Leishmania genus. Notably, search for new, promising and potent anti-Leishmania compounds remains major goal due inefficacy of available drugs used nowadays. In present work, we evaluated effects 1,10-phenanthroline-5,6-dione (phendione) coordinated silver(I), [Ag(phendione)2]ClO4 (Ag-phendione), copper(II), [Cu(phendione)3](ClO4)2·4H2O (Cu-phendione), as potential be in chemotherapy against amazonensis chagasi. The results showed that promastigotes treated with Ag-phendione Cu-phendione presented significant reduction proliferation rate. IC50 values calculated Cu-phendione, respectively, were 7.8 nM 7.5 L. 24.5 20.0 Microscopical analyses revealed several relevant morphological changes promastigotes, such rounding cell body shortening/loss single flagellum. Moreover, treatment promoted alterations unique mitochondrion these parasites, inducing reductions on both metabolic activity membrane parameters. All cellular perturbations induced triggering apoptosis-like death judged (i) increased percentage annexin-positive/propidium iodide negative cells, (ii) augmentation proportion parasites sub-G0/G1 phase (iii) DNA fragmentation. Finally, test intracellular amastigotes; contrarily, molecules well tolerated THP-1 macrophages, which resulted excellent selective index values. Overall, highlight new effective species, are important etiological agents cutaneous (L. amazonensis) visceral chagasi) leishmaniasis global perspective.
Язык: Английский
Процитировано
12
Acta Tropica, Год журнала: 2024, Номер 254, С. 107190 - 107190
Опубликована: Март 18, 2024
Язык: Английский
Процитировано
4
Estudios y Perspectivas Revista Científica y Académica, Год журнала: 2025, Номер 4(4), С. 2464 - 2512
Опубликована: Янв. 22, 2025
El desarrollo de resistencia a los fármacos leishmanicidas representa un reto significativo en el control la leishmaniasis, debido disminución eficacia tratamientos por aparición cepas resistentes. Este estudio tuvo como objetivo analizar las tendencias investigación relacionadas con farmacorresistencia Leishmania, identificando patrones literatura producción científica, autores relevantes y actuales. Se revisaron 672 artículos indexados dos principales bases datos fuentes bibliográficas, posteriormente fueron clasificados, siguiendo metodología PRISMA. En presente trabajo se plantean dar respuestas siguientes interrogantes: 1. ¿Explorar medidas bibliométricas estudios Leishmaniasis su Fármacos? 2. ¿Cuáles son fármacos?, aplicando análisis bibliométrico. Los resultados obtenidos definen 4 áreas críticas fármacos, son: Enfermedades Tropicales Descubrimiento Fármacos, Resistencia Terapéutica Leishmaniasis, Dinámica Molecular Actividad Antileishmanial Simulación Cribado Molecular.
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 155 - 178
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Апрель 23, 2025
Язык: Английский
Процитировано
0
Pathogens, Год журнала: 2025, Номер 14(5), С. 436 - 436
Опубликована: Апрель 30, 2025
Cutaneous leishmaniasis (CL) is a significant public health concern that affects many countries. This disease caused by the protozoan parasite Leishmania spp. and transmitted through sandflies from genus Phlebotomus Lutzomyia. The clinical manifestations of CL can vary, often leading to challenges in accurate diagnosis treatment. In 2022, 51-year-old male patient presented tertiary care hospital Puducherry, India, with progressively worsening facial lesions granulomatous plaques. had recently returned Saudi Arabia, where he likely contracted infection. Before visited been misdiagnosed treated for conditions such as Erysipelas Acute Lupus Erythematosus (ACLE), highlighting diagnostic associated CL. Skin scrapings were subjected real-time PCR, confirming spp.'s presence. Cytological examinations revealed amastigote-like structures within macrophages, thereby establishing identity parasite. For precise species-level identification, PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Sanger sequencing Internal Transcribed Spacer-1 (ITS-1) region performed. Molecular techniques confirmed infection being tropica. Following diagnosis, was successfully Liposomal Amphotericin B, treatment known its efficacy against infections. case underscores critical importance considering cutaneous differential travelers returning endemic areas who present dermatological manifestations. initial misdiagnosis inappropriate highlight need heightened awareness utilization advanced tools identification. Effective timely treatment, demonstrated this case, essential management control disease. report emphasizes necessity vigilance among healthcare providers recognize appropriately address imported cases leishmaniasis.
Язык: Английский
Процитировано
0
Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 12
Опубликована: Май 26, 2025
Leishmaniasis remains a significant global health challenge, with over billion people at risk of infection and limited effective treatment options due to escalating drug resistance. This review explores the underlying mechanisms resistance in Leishmania species, focusing on genomic plasticity as driving factor for survival adaptation. Key mechanisms, including genetic mutations, gene amplification, chromosomal rearrangements, efflux transporters, contribute parasite’s ability evade existing therapies. Advances proteomic studies have provided deeper insights into these pathways, enabling development novel therapeutic strategies. Additionally, this highlights current approaches, combination therapies potential new candidates, that address multidrug explore vulnerabilities . Understanding their clinical implications is essential developing targeted interventions improve outcomes combat leishmaniasis.
Язык: Английский
Процитировано
0
Current Protein and Peptide Science, Год журнала: 2023, Номер 24(3), С. 229 - 239
Опубликована: Фев. 15, 2023
Leishmaniasis is the second deadliest vector-borne, neglected tropical zoonotic disease and found in a variety of clinical forms based on genetic background. Its endemic type present tropical, sub-tropical Mediterranean areas around world which accounts for lot deaths every year. Currently, techniques are available detection leishmaniasis each technique having it's own pros cons. The advancing next-generation sequencing (NGS) employed to find out novel diagnostic markers single nucleotide variants. A total 274 NGS studies European Nucleotide Archive (ENA) portal (https://www.ebi.ac.uk/ena/browser/home) that focused wild-type mutated Leishmania, differential gene expression, miRNA aneuploidy mosaicism by omics approaches. These have provided insights into population structure, virulence, extensive structural variation, including known suspected drug resistance loci, mosaic hybrid formation under stressed conditions inside midgut sandfly. complex interactions occurring within parasite-host-vector triangle can be better understood Further, advanced CRISPR technology allows researchers delete modify individually know importance genes virulence survival disease-causing protozoa. In vitro generation Leishmania hybrids helping understand mechanism progression its different stages infection. This review will give comprehensive picture data various spp. helped reveal effect climate change spread vector, pathogen strategies, emerging antimicrobial importance.
Язык: Английский
Процитировано
6