Exopolysaccharides Producing Bacteria: A Review

Microorganisms, Год журнала: 2023, Номер 11(6), С. 1541 - 1541

Опубликована: Июнь 9, 2023

Bacterial exopolysaccharides (EPS) are essential natural biopolymers used in different areas including biomedicine, food, cosmetic, petroleum, and pharmaceuticals also environmental remediation. The interest them is primarily due to their unique structure properties such as biocompatibility, biodegradability, higher purity, hydrophilic nature, anti-inflammatory, antioxidant, anti-cancer, antibacterial, immune-modulating prebiotic activities. present review summarizes the current research progress on bacterial EPSs properties, biological functions, promising applications various fields of science, industry, medicine, technology, well characteristics isolation sources EPSs-producing strains. This provides an overview latest advances study important industrial xanthan, cellulose, levan. Finally, limitations future directions discussed.

Язык: Английский

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Morphogenesis of bacterial cables in polymeric environments

Science Advances, Год журнала: 2025, Номер 11(3)

Опубликована: Янв. 17, 2025

Many bacteria live in polymeric fluids, such as mucus, environmental polysaccharides, and extracellular polymers biofilms. However, laboratory studies typically focus on cells polymer-free fluids. Here, we show that interactions with shape a fundamental feature of bacterial life—how they proliferate space multicellular colonies. Using experiments, find when polymer is sufficiently concentrated, generically reversibly form large serpentine “cables” proliferate. By combining experiments biophysical theory simulations, demonstrate this distinctive colony morphogenesis arises from an interplay between polymer-induced entropic attraction neighboring their hindered ability to diffusely separate each other viscous solution. Our work thus reveals pivotal role sculpting proliferating colonies, implications for how interact hosts the natural environment, uncovers quantitative principles governing complex environments.

Язык: Английский

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Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures

npj Biofilms and Microbiomes, Год журнала: 2023, Номер 9(1)

Опубликована: Июль 28, 2023

Abstract Pseudomonas aeruginosa forms suspended multicellular aggregates when cultured in liquid media. These may be important disease, and/or as a pathway to biofilm formation. The polysaccharide Psl and extracellular DNA (eDNA) have both been implicated aggregation, but previous results depend strongly on the experimental conditions. Here we develop quantitative microscopy-based method for assessing changes size distribution of over time growing cultures. For exponentially cultures P. PAO1, find that aggregation is mediated by cell-associated Psl, rather than either eDNA or secreted Psl. arise de novo within culture via growth process involves collisions clonal growth, non-producing cells do not aggregate with producers. In contrast, stationary phase (overnight) contain different type aggregate, which mediate cohesion. Our findings suggest physical biological properties very early-stage vs late-stage bacterial

Язык: Английский

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Production of Bacterial Exopolysaccharides: Xanthan and Bacterial Cellulose

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(19), С. 14608 - 14608

Опубликована: Сен. 27, 2023

Recently, degradable biopolymers have become increasingly important as potential environmentally friendly biomaterials, providing a wide range of applications in various fields. Bacterial exopolysaccharides (EPSs) are biomacromolecules, which due to their unique properties found biomedicine, foodstuff, textiles, cosmetics, petroleum, pharmaceuticals, nanoelectronics, and environmental remediation. One the commercial polysaccharides produced on an industrial scale is xanthan. In recent years, its application has expanded significantly. cellulose (BC) another EPS with rapidly increasing applications. Due great prospects for practical application, development highly efficient production remains task. The present review summarizes strategies cost-effective such biomacromolecules xanthan BC demonstrates first time common approaches obtaining new functional materials applications, including wound healing, drug delivery, tissue engineering, remediation, 3D bioprinting. end, we discuss limitations line future research.

Язык: Английский

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Cost-Effective Production of Bacterial Cellulose and Tubular Materials by Cultivating Komagataeibacter sucrofermentans B-11267 on a Molasses Medium

Polymers, Год журнала: 2025, Номер 17(2), С. 179 - 179

Опубликована: Янв. 14, 2025

An original design of a simple bioreactor was used to fabricate two tubular, 200 cm long BC structures by culturing Komagataeibacter sucrofermentans B-11267 on molasses medium. In addition, tubular BC-based biocomposite with improved mechanical properties obtained combining cultivation the medium in situ chemical modification polyvinyl alcohol (PVA). Moreover, present study investigated production K. strain media different concentrations under agitated culture conditions. The dynamics sugar consumption during were studied HPLC. structure and physicochemical characterized FTIR spectroscopy X-ray diffraction (XRD). Thus, findings indicate that B-11267, when cultivated medium, which is such cheap waste product industry, forms significant amount high crystallinity degree. demonstrated great potential for their application biomedicine as artificial blood vessels conduits nerve regeneration.

Язык: Английский

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Interfacial Morphodynamics of Proliferating Microbial Communities

Physical Review X, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 29, 2025

In microbial communities, various cell types often coexist by occupying distinct spatial domains. What determines the shape of interface between such domains—which, in turn, influences interactions cells and overall community function? Here, we address this question developing a continuum model 2D spatially structured with two types. We find that, depending on balance different proliferation rates substrate friction coefficients, domains is either stable smooth or unstable develops fingerlike protrusions. establish quantitative principles describing when these interfacial behaviors arise good agreement both results previous experimental reports as well new experiments performed here. Our work, thus, helps to provide biophysical basis for understanding morphodynamics proliferating communities broader range active systems. Published American Physical Society 2025

Язык: Английский

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Enhanced penetration and biofilm eradication by sophorolipid micelles encapsulating Honokiol: a comprehensive solution for biofilm-associated lung infections

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 3, 2025

Biofilm-associated lung infections, particularly those caused by Staphylococcus aureus (S. aureus), pose significant clinical challenges to conventional therapies. S. Biofilm infections are refractory treatment due the presence of persister bacterial cells and barrier effect unique extracellular polymeric substances (EPS). This study describes development multifunctional micelles, HK-SL Ms, utilizing sophorolipid (SL) encapsulate Honokiol (HK). Ms potently disrupted EPS barrier, killed some internal colonizing bacteria, inhibited further adhesion. Consequently, dynamic cycling biofilms was hindered, achieving a promising removal biofilms. In vitro studies demonstrated that exhibited antimicrobial reduction 6.42 log10CFU/mL. eradicated 71.73% targeting polysaccharides, proteins, viable within biofilm. Additionally, 1.66 log10CFU/mL units were killed. Moreover, 91.10% early biofilm formation obstructing initial adhesion polysaccharides polysaccharide intercellular adhesins (PIA). Thus, reestablishment reinfection could be resolved promisingly. as predominant in acute pneumonia chronic cases, inducing similar inflammation. murine model infected aureus, significantly reduced load lungs, decreased inflammatory factor levels, repaired tissue damage. offers novel strategy for biofilm-associated dispersing removing preventing new infections.

Язык: Английский

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Designing biofilm architecture through depletion-mediated self-assembly and emergent nematic order

Current Opinion in Solid State and Materials Science, Год журнала: 2025, Номер 36, С. 101222 - 101222

Опубликована: Апрель 6, 2025

Язык: Английский

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Moringa oleifera seed preparations containing the lectin WSMoL inhibit growth, cell aggregation, and biofilm production of Listeria monocytogenes

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107600 - 107600

Опубликована: Апрель 1, 2025

Язык: Английский

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Burkholderia cenocepacia and Pseudomonas aeruginosa in dual-species models: Insights into population distribution, antibiotic susceptibility, and virulence

Virulence, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 24, 2025

Multispecies biofilms are communities composed of different microorganisms embedded in an auto-synthesized polymeric matrix. Pseudomonas aeruginosa and Burkholderia cenocepacia two multidrug-resistant biofilm-forming opportunistic pathogens often found the lungs people living with cystic fibrosis. In this context, planktonic, static, dynamic vivo models both species were optimized work to understand their population dynamics, disposition, virulence, antibiotic susceptibility. From coculture work, we determined that B. grows a clustered, aggregative manner at bottom layers biofilms, close contact P. aeruginosa, tends occupy top layers. Their coexistence increases virulence-related gene expression early stages coinfection models, while there was general downregulation genes after longer periods as they eventually reach non-competitive stage during chronic infections. When evaluating antimicrobial susceptibility, decrease tolerance observed when co-cultured. These findings shed light on differential behavior dual-species systems, stressing relevance multispecies studies clinical context.

Язык: Английский

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