bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Фев. 17, 2023
Abstract
Urinary
tract
infections
(UTI)
are
one
of
the
most
common
bacterial
worldwide.
While
overall
infection
course
is
known
on
a
macroscale,
behavior
not
fully
understood
at
cellular
level
and
pathophysiology
during
multispecies
well
characterized.
Here
we
establish
co-infection
models
combined
with
high
resolution
imaging
to
compare
single-
multi-species
bladder
cell
invasion
events
in
three
uropathogens:
uropathogenic
Escherichia
coli
(UPEC),
Klebsiella
pneumoniae
Enterococcus
faecalis
.
all
species
invaded
cells,
under
flow
conditions
Gram-positive
E.
was
significantly
less
invasive
compared
Gram-negative
UPEC
K.
When
introduced
simultaneously
an
experiment,
sometimes
same
cell,
differing
frequencies
suggesting
complex
interactions
between
cells.
Inside
host
observed
encasement
colonies
specifically
by
UPEC.
During
subsequent
dispersal
from
only
bacteria
underwent
infection-related
filamentation
(IRF).
Taken
together,
our
data
suggest
that
invasions
single
cells
frequent
support
earlier
studies
showing
intraspecies
cooperation
biochemical
UTI.
Mucosal Immunology,
Год журнала:
2022,
Номер
15(6), С. 1127 - 1142
Опубликована: Сен. 30, 2022
All
mucosal
surfaces
must
deal
with
the
challenge
of
exposure
to
outside
world.
The
urothelium
is
a
highly
specialized
layer
stratified
epithelial
cells
lining
inner
surface
urinary
bladder,
gruelling
environment
involving
significant
stretch
forces,
osmotic
and
hydrostatic
pressures,
toxic
substances,
microbial
invasion.
bladder
plays
an
important
barrier
role
allows
accommodation
expulsion
large
volumes
urine
without
permitting
components
diffuse
across.
made
up
three
cell
types,
basal,
intermediate,
umbrella
cells,
whose
functions
aid
in
bladder's
mission.
In
this
review,
we
summarize
recent
insights
into
urothelial
structure,
function,
development,
regeneration,
particular
formation
maintenance.
We
briefly
review
diseases
which
involve
discuss
current
human
vitro
models
as
complement
traditional
animal
studies.
Trends in Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Within
both
abiotic
and
host
environments,
bacteria
typically
exist
as
diverse,
multispecies
communities
have
crucial
roles
in
human
health,
agriculture,
industry.
In
these
communities,
compete
for
resources,
competitive
interactions
can
shape
the
overall
population
structure
community
function.
Studying
bacterial
dynamics
requires
experimental
model
systems
that
capture
different
interaction
networks
between
their
surroundings.
We
examine
recent
literature
advancing
such
systems,
including
(i)
silico
models
establishing
theoretical
basis
how
cell-to-cell
influence
level
dynamics,
(ii)
vitro
characterizing
specific
interbacterial
interactions,
(iii)
organ-on-a-chip
revealing
physiologically
relevant
parameters,
spatial
mechanical
forces,
encounter
within
a
host,
(iv)
vivo
plant
animal
connecting
responses
to
interactions.
Each
of
has
greatly
contributed
our
understanding
be
used
synergistically
understand
competition
influences
architecture.
Urinary
tract
infection
is
among
the
most
common
infections
worldwide,
typically
studied
in
animals
and
cell
lines
with
limited
uropathogenic
strains.
Here,
we
assessed
diverse
bacterial
species
a
human
urothelial
microtissue
model
exhibiting
full
stratification,
differentiation,
innate
epithelial
responses,
urine
tolerance.
Several
uropathogens
invaded
intracellularly,
but
also
commensal
Pathogens and Disease,
Год журнала:
2024,
Номер
82
Опубликована: Янв. 1, 2024
Abstract
Urinary
tract
infection
(UTI),
one
of
the
most
common
bacterial
infections
worldwide,
is
a
typical
example
an
that
often
polymicrobial
in
nature.
While
overall
course
known
on
macroscale,
behavior
not
fully
understood
at
cellular
level
and
pathophysiology
during
multispecies
well
characterized.
Here,
using
clinically
relevant
bacteria,
human
epithelial
bladder
cells
urine,
we
establish
co-infection
models
combined
with
high
resolution
imaging
to
compare
single-
multi-species
cell
invasion
events
three
uropathogens:
uropathogenic
Escherichia
coli
(UPEC),
Klebsiella
pneumoniae
Enterococcus
faecalis.
all
species
invaded
cells,
under
flow
conditions
Gram-positive
E.
faecalis
was
significantly
less
invasive
compared
Gram-negative
UPEC
K.
pneumoniae.
When
introduced
simultaneously
experiment,
sometimes
same
cell,
differing
frequencies
suggesting
complex
interactions
between
cells.
Inside
host
observed
encasement
colonies
specifically
by
UPEC.
During
subsequent
dispersal
from
only
bacteria
underwent
infection-related
filamentation
(IRF).
Taken
together,
our
data
suggest
invasions
single
are
frequent
support
earlier
studies
showing
intraspecies
cooperation
biochemical
UTI.
npj Antimicrobials and Resistance,
Год журнала:
2025,
Номер
3(1)
Опубликована: Май 21, 2025
Abstract
Bacterial
infections
remain
a
major
global
health
issue,
with
antimicrobial
resistance
(AMR)
worsening
the
crisis.
However,
treatment
failure
can
occur
even
when
bacteria
show
antibiotic
susceptibility
in
diagnostic
tests.
We
explore
factors
such
as
phenotypic
resilience,
bacterial
lifestyles
biofilms,
and
differences
between
laboratory
tests
real
infection
sites,
highlighting
need
for
improved
platforms
to
better
predict
outcomes,
reviewing
emerging
technologies
aimed
at
improving
testing.
Current Opinion in Nephrology & Hypertension,
Год журнала:
2024,
Номер
33(5), С. 535 - 542
Опубликована: Май 10, 2024
Purpose
of
review
25
years
after
the
discovery
claudins
as
central
constituents
tight
junctions,
“hunter-gatherer
phase”
claudin
research
is
coming
to
an
end.
Deficiency
in
individual
a
cause
rare
hereditary
diseases
well
documented.
However,
knowledge
about
involvement
renal
common
kidney
and
strategies
utilize
or
their
regulators
for
intervention
are
still
scarce.
The
present
summarizes
novel
approaches
address
these
questions.
Recent
findings
Publicly
accessible
omics
data
provide
new
insights
not
only
into
general
expression
patterns
along
nephron,
but
also
sex-specific
differences
dysregulation
injury.
Computational
association
studies
identify
variants
risk
factors
disease
such
nephrolithiasis
loss
filtration
capacity.
establishment
innovative
cell
culture
organoid
models
contributes
better
understanding
junctional
extra-junctional
functions
claudins.
Summary
current
lay
foundation
identification
upstream
thus
development
concepts
treatment
disease.
