Prime-Boost Vaccination Based on Nanospheres and MVA Encoding the Nucleoprotein of Crimean-Congo Hemorrhagic Fever Virus Elicits Broad Immune Responses
Vaccines,
Год журнала:
2025,
Номер
13(3), С. 291 - 291
Опубликована: Март 10, 2025
Background/Objectives:
Crimean–Congo
hemorrhagic
fever
virus
(CCHFV)
is
an
emerging,
widely
distributed
zoonotic
tick-borne
pathogen.
The
causes
severe
disease
in
humans,
and
numerous
wild
domestic
animals
act
as
reservoirs
of
it.
Unfortunately,
there
are
no
effective
therapies
or
safe
vaccines
commercialized
nowadays
for
this
particular
virus.
As
CCHF
(Crimean–Congo
fever)
a
serious
threat
to
public
health,
urgent
need
investigate
the
development
vaccination
strategies
further.
Methods:
In
work,
we
have
employed
two
immunization
platforms
based
on
protein
nanoparticles
modified
vaccinia
Ankara
(MVA)
viral
vector
using
nucleoprotein
(NP)
target
antigen.
humoral
cellular
immune
responses
were
characterized
by
ELISA,
ICS,
cytokine
measurement.
Results:
This
work
shows
that
single
dose
vaccine
candidates
was
not
immunogenic
heterologous
MVA.
A
prime
with
NP
(NS-NP)
boost
MVA-expressing
capable
triggering
significant
levels
against
CCHFV
mice.
Conclusions:
Our
study
NS-NP/MVA-NP
strategy
effectively
elicits
robust
response
mouse
model,
emphasizing
its
potential
protective
approach
lineages.
Язык: Английский
Looking Beyond the Lens of Crimean-Congo Hemorrhagic Fever in Africa
Emerging infectious diseases,
Год журнала:
2024,
Номер
30(7)
Опубликована: Июнь 10, 2024
Crimean-Congo
hemorrhagic
fever
(CCHF)
is
a
lethal
viral
disease
that
has
severe
public
health
effects
throughout
Africa
and
case
fatality
rate
of
10%-40%.
CCHF
virus
was
first
discovered
in
Crimea
1944
since
caused
substantial
burden
Africa.
The
shortage
diagnostic
tools,
ineffective
tick
control
efforts,
slow
adoption
preventive
measures,
cultural
hurdles
to
education
are
among
the
problems
associated
with
continued
transmission.
Progress
preventing
spread
also
hampered
by
dearth
effective
serodiagnostic
testing
for
animals
absence
precise
surveillance
protocols.
Intergovernmental
coordination,
creation
regional
reference
laboratories,
multiinstitutional
partnerships,
investments
healthcare
infrastructure,
vaccine
development,
One
Health
approach
strategic
methods
solving
prevention
challenges.
Coordinated
efforts
financial
commitments
needed
combat
improve
all-around
readiness
newly
developing
infectious
illnesses
Язык: Английский
CHO cells for virus-like particle and subunit vaccine manufacturing
Vaccine,
Год журнала:
2024,
Номер
42(10), С. 2530 - 2542
Опубликована: Март 19, 2024
Chinese
Hamster
Ovary
(CHO)
cells,
employed
primarily
for
manufacturing
monoclonal
antibodies
and
other
recombinant
protein
(r-protein)
therapeutics,
are
emerging
as
a
promising
host
vaccine
antigen
production.
This
is
exemplified
by
the
recently
approved
CHO
cell-derived
subunit
vaccines
(SUV)
against
respiratory
syncytial
virus
(RSV)
varicella-zoster
(VZV),
well
enveloped
virus-like
particle
(eVLP)
hepatitis
B
(HBV).
Here,
we
summarize
design,
production,
immunogenicity
features
of
these
review
most
recent
progress
CHO-derived
in
pre-clinical
clinical
development.
We
also
discuss
challenges
associated
with
production
focus
on
ensuring
viral
clearance
eVLP
products.
Язык: Английский
Efficient Identification of Monoclonal Antibodies Against Rift Valley Fever Virus Using High-Throughput Single Lymphocyte Transcriptomics of Immunized Mice
Antibodies,
Год журнала:
2025,
Номер
14(1), С. 12 - 12
Опубликована: Фев. 4, 2025
Background:
Rift
Valley
fever
virus
(RVFV)
is
a
zoonotic
that
poses
significant
threat
to
both
livestock
and
human
health
has
caused
outbreaks
in
endemic
regions.
In
humans,
most
patients
experience
febrile
illness;
however,
some
patients,
RVF
disease
may
result
hemorrhagic
fever,
retinitis,
or
encephalitis.
While
several
veterinary
vaccines
are
being
utilized
countries,
currently,
there
no
licensed
therapeutics
for
use.
Neutralizing
antibodies
specifically
targeting
vulnerable
pathogen
epitopes
promising
candidates
prophylactic
therapeutic
interventions.
the
case
of
RVFV,
surface
glycoproteins
Gc
Gn,
which
harbor
neutralizing
epitopes,
represent
primary
targets
vaccine
antibody
development.
Methods:
We
report
implementation
advanced
10x
Genomics
technology,
enabling
high-throughput
single-cell
analysis
identification
rare
potent
against
RVFV.
Following
immunization
mice
with
live
attenuated
rMP-12-GFP
successive
Gc/Gn
boosts,
memory
B
cell
populations
(both
general
antigen-specific)
were
sorted
from
splenocytes
by
flow
cytometry.
Deep
sequencing
repertoire
at
resolution,
together
bioinformatic
analyses,
was
applied
BCR
pair
selection
based
on
their
abundance
specificity.
Results:
Twenty-three
recombinant
monoclonal
(mAbs)
selected
expressed,
antigen-binding
capacities
characterized.
About
half
them
demonstrated
specific
binding
cognate
antigen
relatively
high
affinities.
Conclusions:
These
could
be
used
future
development
efficacious
therapeutics,
as
well
studying
virus-neutralizing
mechanisms.
The
current
study,
approach
implemented
RVFV
proteins
demonstrates
effective
applicability
this
technique
discovery
purposes.
Язык: Английский
Editorial: Tick-borne Bunyaviruses: an emerging public health threat
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 13, 2025
Bunyaviruses
comprise
496
di:erent
species
(Chen
et
al.,
2023)
and
the
order
Bunyavirales
was
recently
promoted
to
Bunyaviricetes
class
reflecting
expanding
number
of
bunyaviruses
being
discovered
(Kuhn
2024).
These
viruses
infect
a
variety
hosts
from
plants
insects
rodents
humans.
Many
are
transmitted
humans
via
vectors
such
as
ticks,
mosquitoes
sandflies
for
example.
In
addition,
belonging
spreading
into
new
areas
due
globalization
climate
changes
some
on
World
Health
Organizations
list
prioritized
diseases
(World
Organization,
2025).
Crimean-Congo
hemorrhagic
fever
virus
(CCHFV),
mostly
by
Hyalomma
sp.
is
reported
Southern
Eastern
Europe,
Africa,
Middle
East
Asia,
but
now
also
found
in
France
Spain
(European
Center
Disease
Control,
The
has
been
far
north
Sweden
(Grandi
al.
2020).
Heartland
(HRTV)
South
USA
Amblyomma
americanum
(Dembek
Severe
Fever
with
thrombocytopenia
syndrome
(SFTS),
caused
Dabie
bandavirus
(DBV)
aka
Huaiyangshan
Banyangvirus
can
be
China,
Korea
Japan
vectored
mainly
Haemaphysalis
longicornis,
other
ticks
testudinarium,
Japanese
hard
Rhipicephalus
microplus
(Casel
All
these
share
an
increase
cases
most
probably
increased
human-tick
interactions.
Additionally,
any
attempts
predict
course
rather
di:icult
it
seems
that
treatment
disease
according
prognoses
reflects
crucial
factor
at
early
stage
disease.Since
first
description
SFTS
infection
China
2009
identification
2011
(Yu
2011),
have
represented
upcoming
issue
severeness
its
pandemic
potential.
Although
currently
Japan,
Korea,
Vietnam
Taiwan,
main
vector
tick
H.
longicornis
occur
United
States
America.
Similar
borne
viruses,
very
fast
contact
wildlife
human
population.
Furthermore,
migrating
birds
might
play
key
role
2020).While
primary
vector,
wild
animals
pets
cats
dogs
(Seo
2021).
Also
human-to-human
transmission
bodily
fluids
(Kim
2015).
After
transmission,
incubation
time
approximately
1-2
weeks.
Even
though
patients
asymptomatic,
clinical
symptoms
fever,
gastrointestinal
problems,
leukopenia
Acute
kidney
injury
elevated
liver
enzymes
(alanine
aminotransferase,
aspartate
aminotransferase
alkaline
phosphatase)
additional
symptoms.
However,
diagnose
since
similar
infectious
diseases.
Reported
mortality
rate
di:er
between
6.3-30%
(National
Institute
Infectious
Diseases,
China)
death
primarily
multiple
organ
failure.
Currently
there
no
e:ective
or
vaccine
infection.With
high
rate,
global
well
lack
scarce
knowledge
biomarkers
goes
along
this
SFTS,
di:iculties
mirrors
those
Bunyaviruses.
Thus,
research
regarding
not
only
important
improve
specific
virus,
potentially
derive
useful
information
Bunyaviruses.Six
showcases
outlined
topic
special
edition,
which
help
elucidate
vector-borne
BunyavirusesThe
importance
clearly
seen
bibliometric
analysis
Zhang
Zhang,
who
identified
interest
STFS.
They
clear
upward
trend,
especially
2021
forward,
published
papers
description.
reason
therefor
supposed
based
factors
increases
severity
cases,
more
concern
diseases,
case
reports
higher
demand
prevention
control
measures.
This
work
highlights
current
trend
enables
scientist
remain
focussed
these.One
approaches
fight
against
outcome
Therefore
Liu
analysed
risk
obtained
24
studies
including
4793
establish
prediction
model
chance
survival
patient.
validated
external
cohort.
Six
indicators
were
relevant
parameters:
age,
manifestation,
encephalopathy,
activated
partial
thromboplastin
time,
blood
urea
nitrogen,
serum
creatinine.
study
literature
review
used
obtain
analyse
regard
potential
therefore
represents
step
towards
models.
more,
He
investigated
associated
SFTS.
applied
six
machine
learning
(ML)
methods
set
483
participant´s
data
construct
high-risk
patient
admission.
variables,
days
onset
admission,
cerebral
infarction,
calcium
ions,
creatinine,
creatinine
kinase
isoenzyme
linked
fatal
patients.
authors
state
use
detection
dying
thus
managed
intensely
novelty
here
comparison
tools
prepared
future
accuracy.
Another
understand
pathogenesis
e.g.
understanding
immunosuppression
individuals
SFTSV
support
regimes.
respect,
Sakai
germinal
centre
response
lymph
nodes
pathology
Here,
impact
circulating
discussed
defined
one
disease.
mechanism
behind
thrombocytopenia,
multi-organ
haemorrhage
humans.Beside
implement
curative
treatment,
another
countermeasure
develop
So,
Kim
tested
recombinant
protein
candidates
nucleocapsid
(NP)
surface
glycoproteins
(Gn
Gc)
mouse
model.
assessed
either
alone
combination
results
show
Gc
NP
promising
candidate
so
far.
included
longitudinal
immune
responses
induced
what
necessary
long
protection
infection.
summary,
dynamics
terms
recent
virus.
Based
trends
directions
observed
(Zhang
Zhang).
To
prognosis
obligatory.
While
comparing
(Liu
al.)
(Xia
parameters
defined.
By
using
machines,
will
get
accurate
(He
al.).
(Sakai
essential
treatment.
Vaccines
countermeasures,
protective
al.).Although
guide
while
performing
topics
Bunyavirus.GD:
writing
-original
draft,
SA:
-review
editing,
DH:
editing.
Язык: Английский
Promising Vaccine Formulations for Emerging Infectious Diseases
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4893 - 4893
Опубликована: Май 20, 2025
Emerging
infectious
diseases
(EIDs)
are
one
of
the
greatest
threats
to
human
health
today,
thus
requiring
an
urgent
response.
Vaccines
most
effective
means
preventing
spread
diseases,
and
their
usefulness
in
responding
EIDs
has
been
clearly
proven
through
process
overcoming
global
COVID-19
pandemic.
As
characteristics
various
vaccine
formulations
differ,
it
is
necessary
apply
appropriate
according
EID
response
strategy.
In
this
review,
we
first
consider
which
formulation
suitable
for
vaccines
by
comparing
pros
cons
different
formulations,
then
discuss
utility
mRNA
considered
promising
vaccines.
Язык: Английский
A mRNA Vaccine for Crimean–Congo Hemorrhagic Fever Virus Expressing Non-Fusion GnGc Using NSm Linker Elicits Unexpected Immune Responses in Mice
Viruses,
Год журнала:
2024,
Номер
16(3), С. 378 - 378
Опубликована: Фев. 28, 2024
Crimean–Congo
hemorrhagic
fever
(CCHF),
caused
by
Hemorrhagic
virus
(CCHFV),
is
listed
in
the
World
Health
Organization’s
list
of
priority
diseases.
The
high
fatality
rate
humans,
widespread
distribution
CCHFV,
and
lack
approved
specific
vaccines
are
primary
concerns
regarding
this
disease.
We
used
microfluidic
technology
to
optimize
mRNA
vaccine
delivery
system
demonstrated
that
vaccination
with
nucleoside-modified
CCHFV
encoding
GnNSmGc
(vLMs),
Gn
(vLMn),
or
Gc
(vLMc)
induced
different
immune
responses.
found
both
T-cell
B-cell
responses
vLMc
were
better
than
those
vLMn.
Interestingly,
be
lower
for
vLMs,
which
employed
NSm
link
non-fusion
expression,
compared
vLMc.
In
conclusion,
our
results
indicated
could
a
factor
leads
decreased
host
should
avoided
development
antigens.
Язык: Английский
G-Quadruplexes in hemorrhagic fever viruses of Bunyavirales
Future Virology,
Год журнала:
2024,
Номер
19(5), С. 173 - 183
Опубликована: Март 23, 2024
Aim:
To
investigate
the
presence
of
G-quadruplexes
(G4s)
in
genomes
Crimean-Congo
hemorrhagic
fever
virus
(CCHFV)
and
Rift
Valley
(RVFV),
assess
their
potential
as
targets
for
therapeutic
intervention.
Materials
&
methods:
Bioinformatics
analysis
biophysical
techniques
were
employed
to
identify
characterize
putative
G-quadruplex
sequences
viral
genomes.
Results:
found
CCHFV
RVFV.
Biophysical
confirmed
formation
stable
structures.
Ligand-binding
studies
demonstrated
specific
interactions
between
ligands
G4s.
Conclusion:
Our
findings
provide
evidence
existence
G4s
Bunyavirales
viruses
suggest
intervention
against
fevers.
Язык: Английский