Caracterização de proteínas recombinantes de Rickettsia rickettsii para diagnóstico sorológico em suídeos DOI Creative Commons

Maribel Loza

Опубликована: Авг. 25, 2023

Brazilian Spotted Fever (BSF) is a disease strongly associated with wild boars (Sus scrofa) as its primary hosts, caused by the etiological agent Rickettsia rickettsii.It an endemic zoonosis primarily transmitted to humans through bite of ticks Amblyomma genus, presenting high fatality rate (≥ 50%), especially in southeastern region country.Studies have shown that 72.5% boars, 14.1% hunting dogs, and 14.7% hunters exhibited seropositivity for at least one species Rickettsia.The importance proteins found outer membrane Gram-negative bacteria like R. rickettsii crucial physiological functions, virulence, bacterial resistance, serving cellular support.In this study, we evaluated IgG reactivity rOmpA, rPhenAl, rYJBL using ELISA well-characterized boar serum panel, laboratory-confirmed diagnosis indirect immunofluorescence (IFA).We positivity recombinant samples (97.2% rPhenAl rYJBL; 94.4% rOmpA), indicating these potential antigen candidates various immunoassays be applied detection specific antibodies suids other wildlife naturally exposed sp. immunogens future research development vaccines against rickettsioses.

Язык: Английский

Pathogenic Rickettsia spp. as emerging models for bacterial biology DOI Creative Commons
Brandon Sit, Rebecca L. Lamason

Journal of Bacteriology, Год журнала: 2024, Номер 206(2)

Опубликована: Фев. 5, 2024

Our understanding of free-living bacterial models like

Язык: Английский

Процитировано

9
Cytokine pattern during asymptomatic Anaplasma spp. infections and effect of co-infections by malaria and helminths in schoolchildren of Franceville, southeastern Gabon DOI Creative Commons
Chérone Nancy Mbani Mpega Ntigui, Sandrine Lydie Oyegue-Liabagui,

Jenny Mouloungui-Mavoungou

и другие.

Parasites & Vectors, Год журнала: 2025, Номер 18(1)

Опубликована: Март 27, 2025

Abstract Background Asymptomatic infections by Anaplasma spp. and the basis of immune response during these have not yet been established. This study investigated inflammatory cytokine responses infection in school children effect co-infection with Plasmodium spp . helminths. Methods Blood stool samples were taken from aged 5 to 17 years. Parasitological diagnosis was carried out RDT microscopy, while microscopy PCR used diagnose Plasma for assays using ELISA technique. Results A total 219 included present study, whom 205 infected 14 uninfected. Levels IL-6, IL-22 TGF-β lower only mono-infected but also those co-infected spp., helminths, helminths compared controls. However, higher levels IL-6 observed The latter group had IL-22, IL-10 than In addition, An increase IFN-γ Finally, results showed that febrile afebrile children. Conclusions These suggest downregulates cytokines including might a protective on host, negative effect. Graphical

Язык: Английский

Процитировано

0
TRIM56-mediated production of type I interferon inhibits intracellular replication of Rickettsia rickettsii DOI Creative Commons

Ruxi Cheng,

Chunyu Zhou,

Mingliang Zhao

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 12(4)

Опубликована: Фев. 15, 2024

ABSTRACT Rickettsia rickettsii ( R. ), the causative agent of Rocky Mountain spotted fever (RMSF), is most pathogenic member among spp. Previous studies have shown that tripartite motif-containing 56 (TRIM56) E3 ligase-induced ubiquitination STING important for cytosolic DNA sensing and type I interferon production to induce anti-DNA viral immunity, but whether it affects intracellular replication remains uncharacterized. Here, we investigated effect TRIM56 on HeLa THP-1 cells infected with . We found expression was upregulated in -infected cells, overexpression inhibited , while enhanced TRIM56-silenced host reduced phosphorylation IRF3 increased interferon-β. In addition, mutation ligase catalytic site impairs inhibitory function against cells. Altogether, our study discovers a restriction factor by regulating cGAS-STING-mediated signaling pathway. This gives new evidence role innate immune response bacterial infection provides therapeutic targets RMSF. IMPORTANCE Given ) within genus serves as fever, there growing need explore targets. this study, examined impact observed significant upregulation Remarkably, silencing accompanied STING, along interferon-β production. Notably, TRIM56’s did not impede Collectively, findings provide novel insights into through modulation cGAS-STING

Язык: Английский

Процитировано

0
Molecular detection of rickettsial agents in Amblyomma maculatum ticks (Ixodida: Ixodidae) from Ecuador DOI
Sandra Enríquez, María L. Félix, Sergio E. Bermúdez

и другие.

Veterinary Research Communications, Год журнала: 2024, Номер 48(4), С. 2869 - 2876

Опубликована: Июнь 25, 2024

Язык: Английский

Процитировано

0
Exploring the relationship between gut microbiota, immune characteristics, and female genital tract polyps using genetic evidence DOI Creative Commons
Ran Jia,

Yuhang Fu,

Meng Xue

и другие.

Medicine, Год журнала: 2024, Номер 103(49), С. e40833 - e40833

Опубликована: Дек. 6, 2024

The study investigates the causal relationship between gut microbes and female genital tract polyps, exploring potential mediating role of immune cells via Mendelian randomization (MR) analysis. Our MR was designed following STROBE-MR guidelines. We combined data from a large-scale GWAS meta-analysis, including 731 profiles with microbiology sourced MiBioGen consortium. Univariate employed to identify significantly associated polyps causally. A 2-step analysis utilized investigate cells. Furthermore, we multivariable approach based on Bayesian model averaging (MR-BMA) further assess prioritization microbiota vs characteristics in development polyps. Through univariate analysis, identified significant link 12 microbiota, 31 features, Four pathways involving cells, were among them. MR-BMA indicated marginal inclusion probability (MIP) values exceeding 0.1 for 5 groups: Victivallaceae (model-averaged estimate [MACE] = 0.060, MIP 0.581, P .0089), Ruminococcus gautreuii (MACE 0.052, 0.346, .0640), Lachnoclostrium 0.0380, 0.225, .1875), Alphaprobacter 0.0186, 0.140, .3934), Fusicatenibacter 0.013, 0.110, .5818). Six features exhibit high priority, 0.5, HLA DR CD33+ DR+ CD14dim −0.015, 0.753, .0853), HVEM naive CD4+ T cell 0.024, 0.737, .0053), CD80 CD62L+ plastic cytoplasmic dendritic 0.721, .0228), CD28 activated secret CD4 regulatory 0.0054, 0.706, .3245), CD14+ CD16 monocyte −0.0003, 0.520, .7927), −0.0029, 0.509, .5576). research indicates that exerts an independent influence potentially impacting them various

Язык: Английский

Процитировано

0
Caracterização de proteínas recombinantes de Rickettsia rickettsii para diagnóstico sorológico em suídeos DOI Creative Commons

Maribel Loza

Опубликована: Авг. 25, 2023

Brazilian Spotted Fever (BSF) is a disease strongly associated with wild boars (Sus scrofa) as its primary hosts, caused by the etiological agent Rickettsia rickettsii.It an endemic zoonosis primarily transmitted to humans through bite of ticks Amblyomma genus, presenting high fatality rate (≥ 50%), especially in southeastern region country.Studies have shown that 72.5% boars, 14.1% hunting dogs, and 14.7% hunters exhibited seropositivity for at least one species Rickettsia.The importance proteins found outer membrane Gram-negative bacteria like R. rickettsii crucial physiological functions, virulence, bacterial resistance, serving cellular support.In this study, we evaluated IgG reactivity rOmpA, rPhenAl, rYJBL using ELISA well-characterized boar serum panel, laboratory-confirmed diagnosis indirect immunofluorescence (IFA).We positivity recombinant samples (97.2% rPhenAl rYJBL; 94.4% rOmpA), indicating these potential antigen candidates various immunoassays be applied detection specific antibodies suids other wildlife naturally exposed sp. immunogens future research development vaccines against rickettsioses.

Язык: Английский

Процитировано

0