Ocular surface immune transcriptome and tear cytokines in corneal infection patients

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Апрель 17, 2024

Microbial keratitis is one of the leading causes blindness globally. An overactive immune response during an infection can exacerbate damage, causing corneal opacities and vision loss. This study aimed to identify differentially expressed genes between patients healthy volunteers within cornea conjunctiva elucidate contributing pathways these conditions' pathogenesis. Moreover, it compared conjunctival transcriptomes in corneal-infected cytokine levels tears.

Язык: Английский

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Precision Medicine for Diagnosis of Microbial Keratitis

International Ophthalmology Clinics, Год журнала: 2025, Номер 65(2), С. 20 - 25

Опубликована: Март 21, 2025

Microbial keratitis (MK) is the leading cause of corneal blindness and can be caused by several species bacteria, fungi, or parasites. Accurate timely identification causative microbe critical for initiating targeted antimicrobial anti-inflammatory treatment preventing vision loss. MK diagnosis currently relies on culture microscopy, which have suboptimal yield produce inconclusive results. requires immediate with empiric antimicrobials, followed after identified. The clinical utility therapy in remains controversial likely differs due to host- microbe-specific factors. Newer diagnostic techniques such as whole genome sequencing, metagenomic deep transcriptomics, proteomics potential greatly improve timeliness, accuracy, precision when diagnosing treating MK. Metagenomic sequencing identify pathogenic microbes comparable superior accuracy traditional microbiology techniques, enabling faster more accurate initiation appropriate treatment. Whole isolates has provide patient-specific insights about microbial phylogenetics, resistance genes, virulence factors enable therapy. Characterization host immune response using RNA tear could help inform use tailored therapies prevent damage. Although most these medicine approaches are research phase, they show customize based outcomes.

Язык: Английский

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HDAC6 deacetylates ENKD1 to regulate mitotic spindle behavior and corneal epithelial homeostasis

EMBO Reports, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Corneal diseases can cause severe visual impairment and even blindness, which have been linked to the interruption of corneal epithelial homeostasis. However, underlying molecular mechanisms are largely unknown. In this study, by comparing transcriptomes keratoconus, bacterial keratitis, viral healthy corneas, we found a steady upregulation histone deacetylase 6 (HDAC6) in diseases. Consistently, significant increase HDAC6 was observed mouse corneas with keratitis. Overexpression mice results thickening epithelium. Mechanistic studies reveal that overexpression disrupts mitotic spindle orientation positioning cells. Our data further show deacetylates enkurin domain-containing protein 1 (ENKD1) at lysine 98 thereby impedes its interaction γ-tubulin, restraining centrosomal localization ENKD1 proper function regulating behavior. These findings uncover pivotal role for HDAC6-mediated deacetylation control homeostasis, providing potential therapeutic targets treating

Язык: Английский

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Advances in bioinformatics techniques for ophthalmic diseases

Current Proteomics, Год журнала: 2025, Номер 22(1), С. 100005 - 100005

Опубликована: Фев. 1, 2025

Язык: Английский

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RNA-Seq of an LPS-Induced Inflammation Model Reveals Transcriptional Profile Patterns of Inflammatory Processes

Life, Год журнала: 2024, Номер 14(5), С. 558 - 558

Опубликована: Апрель 26, 2024

The LPS-induced inflammation model is widely used for studying inflammatory processes due to its cost-effectiveness, reproducibility, and faithful representation of key hallmarks. While researchers often validate this using clinical cytokine markers, a comprehensive understanding gene regulatory mechanisms requires extending investigation beyond these Our study leveraged multiple whole-blood bulk RNA-seq datasets rigorously compare the transcriptional profiles well-established with those several human diseases characterized by systemic inflammation. Beyond conventional inflammation-associated systems, we explored additional systems indirectly associated responses (i.e., ISR, RAAS, UPR) customized core list. cross-condition-validation approach spanned four distinct conditions: lupus erythematosus (SLE) patients, dengue infection, candidemia staphylococcus aureus exposure. This analysis approach, utilizing list aimed assess model's suitability underlying triggered diverse factors. resulted in elevated expressions innate immune-associated genes, coinciding suppressed adaptive genes. Also, upregulation genes cellular stresses mitochondrial immune underscored oxidative stress as central driver corresponding both other contexts.

Язык: Английский

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Transcriptomic analysis of Keratoconus in Han Chinese patients: Insights into differential gene expression and ethnic-specific patterns

Experimental Eye Research, Год журнала: 2024, Номер 248, С. 110118 - 110118

Опубликована: Окт. 11, 2024

Язык: Английский

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