Segatella copri Outer-Membrane Vesicles Are Internalized by Human Macrophages and Promote a Pro-Inflammatory Profile
Alison Sepúlveda-Pontigo,
Karissa Chávez-Villacreses,
Cristóbal Madrid-Muñoz
и другие.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3630 - 3630
Опубликована: Апрель 11, 2025
Increased
abundance
of
Segatella
copri
(S.
copri)
within
the
gut
microbiota
is
associated
with
systemic
inflammatory
diseases,
including
rheumatoid
arthritis.
Although
outer-membrane
vesicles
(OMVs)
Gram-negative
bacteria
are
important
players
in
microbiota–host
communication,
effect
S.
copri-derived
OMVs
on
immune
cells
unknown.
Macrophages
engulf
and
eliminate
foreign
material
conditioned
by
environmental
signals
to
promote
either
homeostasis
or
inflammation.
Thus,
we
aimed
explore
impact
copri-OMVs
human
macrophages
vitro,
employing
THP-1
monocyte-derived
macrophage
models.
The
uptake
DiO-labeled
into
was
monitored
confocal
microscopy
flow
cytometry.
Furthermore,
phenotype
cytokine
secretion
naïve
(M0),
pro-inflammatory
(M1),
anti-inflammatory
(M2)
analyzed
cytometry
ELISA,
respectively.
We
show
that
enter
through
macropinocytosis
clathrin-dependent
mechanisms.
copri-OMVs,
but
not
parental
bacterium,
induced
a
dose-dependent
increase
expression
M1-related
surface
markers
M0
M2
activated
large
amounts
cytokines
M1
macrophages.
These
results
highlight
an
role
promoting
responses,
which
might
contribute
diseases.
Язык: Английский
Rapid and receptor-free Prussian blue electrochemical sensor for the detection of pathogenic bacteria in blood
Bioelectrochemistry,
Год журнала:
2025,
Номер
163, С. 108902 - 108902
Опубликована: Янв. 9, 2025
Язык: Английский
Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
There
is
a
widespread
distribution
of
gram-negative
bacteria
worldwide,
which
are
responsible
for
the
deaths
numerous
patients
each
year.
The
illnesses
they
cause
can
be
localized
and
systemic,
these
possess
several
key
virulence
factors
that
contribute
to
their
pathogenicity.
In
recent
years,
distinct
mechanisms
pathogenesis
have
evolved
remain
largely
unknown
scientists
medical
experts.
Among
these,
outer
membrane
vesicles
(OMVs)
undoubtedly
one
most
significant
influencing
virulence.
OMVs
contain
various
bacterial
compounds
diverse
effects
on
host
organisms
immune
system,
potentially
exacerbating
disease
inflammation
while
evading
responses.
This
review
comprehensively
examines
role
in
pathogenesis,
interaction
with
cells,
potential
biomedical
applications.
Understanding
molecular
governing
OMV
biogenesis
function
could
pave
way
novel
antimicrobial
strategies
therapeutic
interventions.
Язык: Английский
Probiotics against Oxidative Stress
IntechOpen eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 19, 2024
Probiotics,
as
host-compatible
microorganisms,
possess
antioxidant
properties
capable
of
inhibiting
the
growth
harmful
bacteria,
improving
digestion,
and
altering
balance
intestinal
microflora.
Lactic
acid
bacteria
(LAB)
are
among
most
beneficial
probiotics.
These
microbiotas
not
only
play
a
vital
role
in
nutrient
uptake
production
but
also
modulate
gastrointestinal
tract
maturation,
immune
system
function,
infection
resistance,
host
metabolism.
Antioxidants
components
that
elicit
oxidation-reduction
homeostasis.
The
term
“oxidative
stress”
refers
to
physiological
imbalance
homeostasis,
resulting
intracellular
hyperproduction
reactive
oxygen
species
(ROS).
Retrospectively,
capacity
LAB
has
been
confirmed.
consumption
demonstrated
be
healthfully
efficient
managing
ROS-related
disorders
affect
nervous,
cardiovascular,
respiratory,
gastrointestinal,
urogenital,
reproductive,
metabolic
systems,
well
mental
disorders.
Furthermore,
various
cancers
progress
due
ROS
triggers.
This
chapter
reviews
capability
against
oxidative
stress
clinical
trials
with
usage.
Язык: Английский