Fitness Burden for the Stepwise Acquisition of First- and Second-Line Antimicrobial Reduced-Susceptibility in High-Risk ESKAPE MRSA Superbugs
Antibiotics,
Год журнала:
2025,
Номер
14(3), С. 244 - 244
Опубликована: Фев. 28, 2025
Background:
The
fitness
costs
(FCs)
of
antimicrobial
resistance
(AMR)
are
crucial
issues
in
onset,
spread,
and,
consequently,
public
health.
In
Staphylococcus
aureus,
AMR
can
induce
significant
FCs
due
to
slow
growth,
low
competitiveness,
and
virulence.
Here,
we
investigated
the
genomics
emerging
for
progressively
acquiring
daptomycin
(DAP)
glycopeptide
(GLY)
reduced
susceptibility
MRSA.
Methods:
Genomics
was
carried
out
using
Illumina-MiSeq
Whole-genome
sequencing
bioinformatics.
biological
isogenic
MRSA
strain
pairs
DAP
GLY-reduced
susceptibility,
under
DAP/GLY
mono
or
combined
therapy,
were
performed
by
in-vitro
independent
competitive
mixed
phenotypic
virulence
analysis,
in-vivo
G.
mellonella
larvae
killing.
Results:
evidenced
four
different
extremely
resistant
high-risk
clones,
i.e.,
ST-5
N315
HA-MRSA,
ST-398
LA-MRSA,
ST-22
USA-100
HA-EMRSA-15,
ST-1
MW2
CA-MRSA.
In-vitro
assays
revealed
lower
virulence,
predominantly
Galleria
killing
ability,
DAP-S
hGISA,
DAP-R
GSSA,
GISA
strains.
Conclusions:
occurrence
conferred
increasing
FCs,
paid
as
a
gradual
reduction
growth
performance.
Язык: Английский
Synergistic removal of Staphylococcus aureus biofilms by using a combination of phage Kayvirus rodi with the exopolysaccharide depolymerase Dpo7
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 7, 2024
Bacteriophages
have
been
shown
to
penetrate
biofilms
and
replicate
if
they
find
suitable
host
cells.
Therefore,
these
viruses
appear
be
a
good
option
tackle
the
biofilm
problem
complement
or
even
substitute
more
conventional
antimicrobials.
However,
in
order
successfully
remove
biofilms,
particular
mature
phages
may
need
administered
along
with
other
compounds.
Phage-derived
proteins,
such
as
endolysins
depolymerases,
offer
safer
alternative
compounds
era
of
antibiotic
resistance.
Язык: Английский