Rapid and precise identification of cervicothoracic necrotizing fasciitis caused by Prevotella and Streptococcus constellatus by using Nanopore sequencing technology: a case report DOI Creative Commons

Manna Zhao,

Xuejun Leng,

Jie Xu

и другие.

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Окт. 21, 2024

Introduction Cervicothoracic necrotizing fasciitis (CNF) is one form of soft-tissue infections, which could lead to patient demise during short course. Therefore, early recognition and immediate treatment contribute promising prognosis patients. Case presentation A 58-year-old diabetic presented with a sore throat progressive irritation the neck chest for 4 days. The initial diagnosis was considered be infection clinician gave empirical anti-infectious medication expectant treatment. During course disease, surgical incision performed relieve suffocation shortness breath. drainage fluids were detected microbiological culture molecular sequencing. Nanopore sequencing technology (NST) helped identify coinfection Streptococcus constellatus Prevotella spp., not recognized original period 15 precise identification pathogen supported guide pharmacologic meropenem linezolid. Ultimately, combined observation post-surgical pathological examination, diagnosed as CNF, much more acute serious than normal infections. has been successfully treated prompt antimicrobial appropriate debridement. Conclusion This case CNF type 2 diabetes, recovered after microbial detection, treatment, intervention. It highlights importance recognizing by applying rapid including NST, in infectious disease.

Язык: Английский

Exploring the clinical and diagnostic value of metagenomic next-generation sequencing for urinary tract infection: a systematic review and meta-analysis DOI Creative Commons
Sike He, Haolin Liu, Xu Hu

и другие.

BMC Infectious Diseases, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 18, 2024

A new pathogen detection tool, metagenomic next-generation sequencing (mNGS), has been widely used for infection diagnosis, but the clinical and diagnostic value of mNGS in urinary tract (UTI) remains inconclusive. This systematic review with meta-analysis aimed to investigate efficacy treating UTIs.

Язык: Английский

Процитировано

3
Clinical value of Metagenomic Next- and Third-Generation Sequencing applied in ultrasound-guided puncture biopsy for diagnosing lymph node tuberculosis DOI Creative Commons

Ting Lin,

Yuehui Yu, Jiaqi Luo

и другие.

Journal of Microbiology Immunology and Infection, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The aim of this study was to assess the clinical utility metagenomic next-generation sequencing (mNGS) and third-generation (mTGS) in diagnosing cervical lymph node tuberculosis through analysis tissue. This recruited 101 patients with suspected took samples under ultrasound guidance. Parallel culture, X-pert, mNGS mTGS workflows each sample were performed. Taking diagnosis as gold standard, We comparatively compared performance four methods. Clinically, 76 cases diagnosed 20 non-lymph tuberculosis. Compared diagnosis, sensitivities 89.47 % 82.89 %, respectively, significantly higher than those culture X-pert which 43.28 68.42 respectively (P < 0.05). specificity reached 100 while 93.75 95 respectively. And alone identified 7 species bacteria, 3 viruses, 5 fungi, more mixed infections. Particularly, besides Mycobacterium detection, may be superior for detection fungi. Pathogen identification is less affected by previous anti-tuberculosis drug usage. play a crucial role rapid accurate treatment on Cervical

Язык: Английский

Процитировано

0
Diagnostic accuracy of nanopore sequencing for detecting Mycobacterium tuberculosis and drug-resistant strains: a systematic review and meta-analysis DOI Creative Commons

Timothy Hudson David Culasino Carandang,

Dianne Jaula Cunanan,

Gail S Co

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 4, 2025

Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) infection, remains a significant public health threat. The timeliness, portability, and capacity of nanopore sequencing for diagnostics can aid in early detection drug susceptibility testing (DST), which is crucial effective TB control. This study synthesized current evidence on the diagnostic accuracy technology detecting MTB its DST profile. A comprehensive literature search PubMed, Scopus, MEDLINE, Cochrane, EMBASE, Web Science, AIM, IMEMR, IMSEAR, LILACS, WPRO, HERDIN Plus, MedRxiv, BioRxiv was performed. Quality assessed using Assessment Diagnostic Accuracy Studies-2 tool. Pooled sensitivity, specificity, predictive values (PV), odds ratio (DOR), area under curve (AUC) were calculated. Thirty-two studies included; 13 addressed only, 15 focused 4 examined both DST. No used Flongle or PromethION. Seven eligible meta-analysis five DST; GridION only while those profile MinION only. Our results indicate that device has high sensitivity [88.61%; 95% CI (83.81-92.12%)] specificity [93.18%; (85.32-96.98%)], positive value [94.71%; (89.99-97.27%)], moderately negative [84.33%; (72.02-91.84%)], excellent DOR [107.23; (35.15-327.15)] AUC (0.932) MTB. Based AUC, excelled pyrazinamide rifampicin resistance; however, it underperformed isoniazid ethambutol resistance. Additional will be needed to provide more precise estimates MinION's drug-resistance, as well resistance pyrazinamide, streptomycin, ofloxacin. Studies bedaquiline, pretomanid, linezolid are lacking. Subgroup analyses suggest overall tends higher with prospective design use standards other than CSTB (Chinese national standard diagnosing TB). Sensitivity reveal retrospective design, GridION, Illumina whole-genome (WGS) decrease any drug-resistant Findings from types analyses, should interpreted caution because low number uneven distribution each subgroup.

Язык: Английский

Процитировано

0
Performance of metagenomic Next-Generation Sequencing and metagenomic Nanopore Sequencing for the diagnosis of tuberculosis in HIV-positive patients DOI Creative Commons
Jing Yuan,

L Wang,

Wei Zhang

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Авг. 21, 2024

Background Patients who were infected by the Human Immunodeficiency Virus ( HIV) could have weakened immunity that is complicated opportunistic infections, especially for Mycobacterium tuberculosis (MTB). Notably, HIV-MTB co-infection will accelerate course of disease progress and greatly increase mortality patients. Since traditional diagnostic methods are time-consuming low sensitivity, we aim to investigate performance mNGS (metagenomic Next-Generation Sequencing) mNPS NanoPore rapid diagnosis in HIV-infected Methods The 122 patients enrolled retrospective analysis. All underwent microbiological tests, mNGS, (or) tests. clinical comprehensive was used as reference standard compare culture, on tuberculosis. We also value mixed-infection. Furthermore, treatment adjustment directed analyzed. Results Compared with composite standard, culture showed 42.6% sensitivity 100% specificity, OMT(other testing) had 38.9% specificity. 58.6% 96.8% 68.0% proportion mixed-infection cases (88.9%) TB group higher than those non-TB (54.8%) more competitive compared methods. there 63 (69.2%) 36 (63.2%) achieved effective after receiving detection respectively. Conclusion Our study indicated high specificity methods, seems better mNGS. Moreover, apparent advantages detecting mixed infection. mNGS-directed medication outcomes lower immunity.

Язык: Английский

Процитировано

2
Diagnostic Accuracy of Nanopore Sequencing for Detecting Mycobacterium tuberculosis and Drug-Resistant Strains: A Systematic Review and Meta-Analysis DOI Creative Commons

Timothy Hudson David Culasino Carandang,

Dianne Jaula Cunanan,

Gail S Co

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

ABSTRACT Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) infection, remains a significant public health threat. The timeliness, portability, and capacity of nanopore sequencing for diagnostics can aid in early detection drug susceptibility testing (DST), which is crucial effective TB control. This study synthesized current evidence on the diagnostic accuracy technology detecting MTB its DST profile. A comprehensive literature search PubMed, Scopus, MEDLINE, Cochrane, EMBASE, Web Science, AIM, IMEMR, IMSEAR, LILACS, WPRO, HERDIN Plus, MedRxiv, BioRxiv was performed. Quality assessed using Assessment Diagnostic Accuracy Studies-2 tool. Pooled sensitivity, specificity, predictive values (PV), odds ratio (DOR), area under curve (AUC) were calculated. Thirty-two studies included; 13 addressed only, 15 focused 4 examined both DST. No used Flongle or PromethION. Seven eligible meta-analysis five DST; GridION only while those profile MinION only. Our results indicate that device has high sensitivity [88.61%; 95% CI (83.81–92.12%)] specificity [93.18%; (85.32–96.98%)], positive value [94.71%; (89.99–97.27%)], moderately negative [84.33%; (72.02–91.84%)], excellent DOR [107.23; (35.15–327.15)] AUC (0.932) MTB. Based AUC, excelled pyrazinamide rifampicin resistance; however, it underperformed isoniazid ethambutol resistance. Additional will be needed to provide more precise estimates MinION’s drug-resistance, as well resistance pyrazinamide, streptomycin, ofloxacin. Studies bedaquiline, pretomanid, linezolid are lacking.

Язык: Английский

Процитировано

0
Rapid and precise identification of cervicothoracic necrotizing fasciitis caused by Prevotella and Streptococcus constellatus by using Nanopore sequencing technology: a case report DOI Creative Commons

Manna Zhao,

Xuejun Leng,

Jie Xu

и другие.

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Окт. 21, 2024

Introduction Cervicothoracic necrotizing fasciitis (CNF) is one form of soft-tissue infections, which could lead to patient demise during short course. Therefore, early recognition and immediate treatment contribute promising prognosis patients. Case presentation A 58-year-old diabetic presented with a sore throat progressive irritation the neck chest for 4 days. The initial diagnosis was considered be infection clinician gave empirical anti-infectious medication expectant treatment. During course disease, surgical incision performed relieve suffocation shortness breath. drainage fluids were detected microbiological culture molecular sequencing. Nanopore sequencing technology (NST) helped identify coinfection Streptococcus constellatus Prevotella spp., not recognized original period 15 precise identification pathogen supported guide pharmacologic meropenem linezolid. Ultimately, combined observation post-surgical pathological examination, diagnosed as CNF, much more acute serious than normal infections. has been successfully treated prompt antimicrobial appropriate debridement. Conclusion This case CNF type 2 diabetes, recovered after microbial detection, treatment, intervention. It highlights importance recognizing by applying rapid including NST, in infectious disease.

Язык: Английский

Процитировано

0