Biochemical and Biophysical Research Communications,
Год журнала:
2024,
Номер
744, С. 151206 - 151206
Опубликована: Дек. 19, 2024
Streptococcus
oralis
is
a
commensal
oral
bacterium
that
acts
as
an
opportunistic
pathogen,
causing
systemic
diseases,
such
infective
endocarditis
and
aspiration
pneumonia.
However,
the
specific
molecular
mechanisms
underlying
its
transition
from
to
pathogenic
state
remain
unclear.
In
this
study,
further
elucidate
virulence
expression,
we
identified
characterized
cell
surface-associated
ecto-5'-nucleotidase
(Nt5e)
in
S.
oralis.
Biochemical
analysis
revealed
Nt5e
metal-dependent
enzyme
dephosphorylating
ATP
producing
adenosine,
immunosuppressive
molecule
inhibits
macrophage
activation.
Additionally,
was
critical
regulator
of
innate
immunity,
particularly
inflammasome
activation,
via
environmental
metabolism.
Analysis
isogenic
nt5e
deletion
mutant
complemented
strain
played
crucial
role
degrading
extracellular
ATP.
The
Nt5e-orchestrated
mechanism
possibly
maintained
host-bacteria
homeostasis
under
normal
conditions,
whereas
dysregulation
facilitated
pathogenicity
circumstances.
Our
study
provides
new
insights
into
by
which
commensals
modulate
host
immune
responses
highlights
potential
therapeutic
target
for
oralis-associated
diseases.
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 408 - 408
Опубликована: Фев. 13, 2025
Gingivitis
and
periodontitis
are
prevalent
periodontal
conditions
associated
with
dysbiosis
of
the
oral
cavity,
which
leads
to
inflammation
bleeding
gums,
loss
tooth
attachment,
degradation
underlying
bone
structure.
The
standard
adjunctive
treatment
for
conditions,
chlorhexidine
mouthwash,
is
effective
but
several
side
effects.
Probiotics
have
been
explored
as
an
alternative
solution
that
promotes
health
by
restoring
homeostasis
in
cavity.
This
review
presents
a
summary
clinical
trials
using
Darolac®
(Oralis
SB®)
probiotic
formulation
(Lactobacillus
acidophilus
Rosell®-52,
Lactobacillus
rhamnosus
Rosell®-11,
Bifidobacterium
longum
Rosell®-175
Saccharomyces
boulardii
CNCM
I-1079)
mouthwash
support
maintenance
or
restoration
its
balance.
In
reviewed
studies,
compared
placebo
other
common
solutions
including
mouthwash.
Studies
show
even
superior
available
solutions,
supports
use
adjuvant
health.
effects
on
reduction
pathogens
markers
reviewed,
association
between
periodontitis,
inflammation,
systemic
diseases,
well
their
implications
probiotics
field,
discussed.
Pharmaceutics,
Год журнала:
2025,
Номер
17(4), С. 463 - 463
Опубликована: Апрель 2, 2025
Background:
Cannabidiol
(CBD)
is
a
natural
compound
from
the
Cannabis
sativa
L.
plant,
which
has
anti-inflammatory,
anti-nociceptive,
neuroprotective,
and
antibacterial
activities.
Objective:
The
aim
of
this
study
was
to
develop
sustained-release
device
CBD
that
can
provide
an
effect
against
Gram-positive
bacteria
Streptococcus
mutans
Staphylococcus
aureus
for
extended
periods
time.
Methods:
incorporated
into
biodegradable
PURASORB
5010
or
7510
DL-lactide/glycolide
polymers
using
either
dimethylsulfoxide
(DMSO)
acetone
as
solvent,
dried
polymer
scaffolds
were
exposed
daily
fresh
culture
bacteria.
bacterial
growth
determined
by
optical
density,
metabolic
activity
biofilms
MTT
assay.
Biofilm
formation
on
visualized
HR-SEM.
Its
anti-inflammatory
measuring
IL-6
release
LPS-stimulated
RAW
264.7
macrophages
ELISA.
Cell
cytotoxicity
normal
Vero
epithelial
cells
gas
chromatography–mass
spectrometry
(GC-MS).
Results:
5010/CBD
had
S.
UA159,
ATCC25923,
clinical
isolate
multidrug-resistant
(MDRSA
CI-M)
strain
tested
period
up
17
days.
7510/CBD
also
activity,
but
overall,
it
less
effective
than
over
addition
PEG400
copolymers
significantly
increased
not
5010/CBD.
sufficient
reduce
LPS-induced
secretion
macrophages,
importantly,
cytotoxic
cells.
found
be
between
1.12
9.43
µg/mL,
far
below
dose
25
µg/mL.
Conclusions:
incorporation
used
prepare
devices
medical
purposes
where
combined
activities
are
desirable.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 22, 2024
Stroke
is
a
devastating
disease
and
ranks
as
the
second
leading
cause
of
death
disability
globally.
Several
studies
have
shown
that
preceding
infection
(PI)
upper
respiratory
tract
are
strongly
associated
with
acute
ischemic
stroke
(AIS).
However,
clinical
implications
underlying
pathological
mechanisms
remain
unclear.
In
this
study,
16S
rRNA
gene
sequencing
was
employed
to
compare
structural
characteristics
oropharyngeal
gut
microbiota
in
AIS
patients
or
without
PI
normal
controls
(NCs;
30
cases
each),
systemic
inflammatory
markers
were
detected
explore
relationship
between
infections
(URTIs)
subsequent
severity
functional
outcome
potential
mechanism.
We
found
AIS-PI
exhibited
elevated
serum
WBC,
NE,
CRP,
Hcy
levels,
well
higher
90-day
mRS
score.
Oropharyngeal
analysis
showed
increased
microbial
richness
sequence.
Principal
coordinate
demonstrated
significant
differences
composition
among
three
groups.
patients,
Megamonas,
Megasphaera,
Ruminococcaceae
UCG
004,
Rothia,
Streptococcus
significantly
enriched
gut.
Opportunistic
pathogens,
including
Thermus,
uncultured
Veillonella
sp.,
Oribacterium
sinu,
be
oropharynx.
The
dysregulated
positively
correlated
markers,
severity,
poor
prognosis.
contrast,
short-chain
fatty
acid-producing
bacteria
Eisenbergiella,
bacterium
NLAE,
Fusicatenibacter,
Ruminococcaceae,
Faecalibacterium
NCs.
Their
abundances
negatively
Our
findings
suggest
PIs
may
contribute
short-term
by
causing
disturbance
promoting
inflammation
levels.
The
impact
of
sports
on
oral
health
has
been
the
subject
extensive
investigation,
with
majority
studies
indicating
a
deterioration
in
health.
However,
composition
microbiota
athletes
and
its
remains
unclear.
objective
this
review
is
to
investigate
potential
correlation
between
athletic
activity
alterations
microbiota.
A
comprehensive
electronic
search
was
conducted
up
November
2024
across
three
different
databases
(PubMed,
Cochrane
Library,
Scopus)
identifying
that
evaluate
association
physical
activity.
Two
independent
blinding
authors
were
involved
study
selection,
data
extraction,
bias
assessment
using
National
Institutes
Health's
(NIH)
quality
tools.
total
147
records
screened,
five
eligible
included.
Recent
have
demonstrated
individuals
who
engage
regular
exhibit
distinctive
microbial
comparison
those
sedentary
lifestyles
or
low
levels
Three
athlete's
modified,
an
increase
genera
Rothia,
Stenotrophomonas,
Veillonella,
decrease
genus
Gemella.
Streptococcus
often
modified
according
four
studies.
This
provided
analysis
scientific
evidence
modified.
But
date,
there
no
clearly
determine
these
variations.
More
homogeneous
limitation
are
needed
better
understand
link
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 361 - 361
Опубликована: Фев. 7, 2025
The
use
of
antibiotics
has
led
to
the
emergence
antibiotic
resistance,
posing
significant
challenges
in
prevention,
control,
and
treatment
microbial
diseases,
while
threatening
public
health,
environment,
food
safety.
In
this
study,
resistance
phenotypes
genotypes
56
endophytic
bacteria
isolates
from
three
species
wild
edible
fungi
Yunnan
were
analyzed
using
Kirby-Bauer
disk
diffusion
method
PCR
amplification.
results
revealed
that
all
sensitive
ofloxacin,
but
was
observed
against
17
other
antibiotics.
Specifically,
55,
53,
51
exhibited
amoxicillin,
penicillin,
vancomycin,
respectively.
Antibiotic
gene
(ARG)
detection
indicated
sulfonamide
sul1
had
highest
rate
(53.57%).
Excluding
ARG
not
detected,
lowest
rates
sul2
sul3
genes,
both
at
1.79%.
Among
six
tetracycline
only
tetK
tetM
detected.
For
β-lactam
antibiotics,
blaTEM,
blaVIM,
blaSHV
genes
present,
blaOXA
absent.
aminoglycoside
aadB
for
aac(3′)-IIa,
acrB,
aadA1
3.57%,
1.79%,
37.5%,
chloramphenicol
Cat
detected
a
14.29%,
whereas
floR
polypeptide
VanC
with
EmgrB
All
quinolone
8.92%
GyrA,
39.29%
GyrB,
37.5%
ParC.
Through
phylogenetic
analysis,
12
are
closely
related
ten
common
foodborne
pathogenic
further
selected
whole-genome
sequencing
assembly.
Gene
annotations
each
isolate
contained
more
than
15
ARGs
over
30
virulence
factors.
Notably,
higher
genotypes,
highlighting
importance
studying
phenotypic
lacks
identifiable
ARGs.
This
study
enriches
research
on
endophytes
provides
new
data
ecology
research.
It
also
offers
critical
insights
monitoring
potentially
sources,
contributing
effective
strategies
ecological
protection,
sustainable
agricultural
development,
health
security.
Tooth
extraction,
while
essential
for
oral
health,
can
contribute
to
systemic
complications,
particularly
increasing
cardiovascular
risk
due
bacterial
translocation
and
inflammation.
Periodontal
disease,
a
major
cause
of
tooth
loss,
exacerbates
this
by
serving
as
reservoir
pathogenic
bacteria
that
enter
the
bloodstream
during
extractions.
This
review
explores
mechanisms
linking
post-extraction
infections
diseases
evaluates
role
nanotechnology
in
mitigating
these
risks.
Advances
antimicrobial
nanoparticles,
nanodrug
delivery
systems,
biosensors
offer
promising
solutions
infection
control
targeted
drug
delivery.
These
innovations
enhance
healing
potentially
reducing
However,
challenges
remain
optimizing
material
biocompatibility,
ensuring
clinical
efficacy,
addressing
translational
hurdles.
underscores
need
interdisciplinary
research
integrate
into
dental
practice,
ultimately
improving
both
health
outcomes.
