Early Diagnosis and Monitoring of Adaptive Immune Response in a Cohort of Mild Mpox Patients During the 2022 Wave DOI Creative Commons
Sara Caldrer, Silvia Accordini,

Annalisa Donini

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 355 - 355

Опубликована: Фев. 6, 2025

Our study wanted to describe the kinetics of serological and adaptive immune responses in mpox patients. Methods: Fourteen patients with laboratory-confirmed were tested at different time points after symptom onset. An immunofluorescence assay was performed evaluate seroconversion specific IgA, IgM, IgG. Moreover, characterization immunological profile T- B-cells performed. Results: The antibody revealed faster more effective IgA than IgM. we detected an increase Active memory B cells CD8+ early phases infection, a reduction CD4+ T-cells respect controls found presence higher levels Treg HIV+ phase infection. Conclusion: data highlight relevance testing onset, suggesting possible role as marker diagnosis, especially close contact subjects. Furthermore, maturation states effector patients, together high levels, may lead us better understand MPXV-HIV co-infection identify potential cellular markers monitor excessive activation involved disease progression.

Язык: Английский

Complete Genome Sequencing, Annotation, and Mutational Profiling of the Novel Clade I Human Mpox Virus, Kamituga Strain DOI Creative Commons

Leandre Murhula Masirika,

Anuj Kumar,

Mansi Dutt

и другие.

The Journal of Infection in Developing Countries, Год журнала: 2024, Номер 18(04), С. 600 - 608

Опубликована: Апрель 30, 2024

Introduction: Human Mpox (formerly monkeypox) infection is an emerging zoonotic disease caused by the virus (MPXV). We describe complete genome annotation, phylogeny, and mutational profile of a novel, sustained Clade I outbreak in city Kamituga Eastern Democratic Republic Congo (DRC). Methodology: A cross-sectional, observational, cohort study was performed among patients all ages admitted to Hospital with symptoms between late September 2023 January 2024. DNA isolated from swabbed lesions sequenced followed phylogenetic analysis, profiling. Results: ongoing Kamituga, South Kivu Province, Congo. Whole-genome sequencing viral RNA samples revealed, on average, 201.5 snps, 28 insertions, 81 deletions, 2 indels, 312.5 total variants, 158.3 amino acid changes, 81.66 intergenic 72.16 synonymous mutations, 106 missense 41.16 frameshift 3.33 inframe deletions across six samples. By assigning mutations at proteome level for MPXV sequences, we observed that seven proteins, namely, C9L (OPG047), I4L (OPG080), L6R (OPG105), A17L (OPG143), A25R (OPG151), A28L (OPG153), B21R (OPG210) have emerged as hot spot based consensuses acids substitutions. Based outcome found deletion D14L (OPG032) gene Following analysis whole assembly, determined this cluster infections genetically distinct previously reported outbreaks, thus propose represents novel subgroup (subgroup VI) MPXV. Conclusions: Here report first time. This presents oubtreaks, suggesting (we term VI). These findings underscore need vigilance continued threats endemic regions.

Язык: Английский

Процитировано

21
Unveiling the Global Surge of Mpox (Monkeypox): A comprehensive review of current evidence DOI Creative Commons
Phelipe Magalhães Duarte, Ridwan Olamilekan Adesola, Swagatika Priyadarsini

и другие.

The Microbe, Год журнала: 2024, Номер 4, С. 100141 - 100141

Опубликована: Авг. 17, 2024

Monkeypox, now named mpox, has emerged as a significant public threat, evidenced by the 2022 outbreak affecting over seventy countries globally. This infectious disease presents debilitating symptoms, including painful skin rashes, mucosal lesions, enlarged lymph nodes, and fever. The situation intensifies with concerns about novel transmission route, specifically through sexual contact, evolution of more transmissible strains. Complicating matters further is documented spillback mpox from humans to animals, raising potential for new animal reservoirs. study utilized systematic approach gather, analyse, interpret data regarding global outbreaks, phylogenomics, human APOBEC3 enzyme activity, antiviral resistance issues, application One Health intervention. Emphasizing covers various aspects, zoonotic origins, pathogenesis, changing epidemiological landscapes, phylogenomic diversity, clade dynamics. review underscores crucial role collaboration in understanding combatting making it valuable resource shaping effective prevention control measures on scale.

Язык: Английский

Процитировано

21
Dynamics of Mpox in an HIV endemic community: A mathematical modelling approach DOI Creative Commons
Andrew Omame, Sarafa A. Iyaniwura, Qing Han

и другие.

Mathematical Biosciences & Engineering, Год журнала: 2025, Номер 22(2), С. 225 - 259

Опубликована: Янв. 1, 2025

<p>During the 2022 monkeypox (Mpox) outbreak in non-endemic countries, sexual transmission was identified as dominant mode of transmission, and particularly affected community men who have sex with (MSM). This experienced highest incidence Mpox cases, exacerbating public health burden they already face due to disproportionate impact HIV. Given simultaneous spread HIV within MSM community, it is crucial understand how these diseases interact. Specifically, since endemic this population, understanding its influence on control essential. In study, we analyze a mechanistic mathematical model explore potential dynamics community. The considered work incorporates two diseases, including antiretroviral therapy (ART) for We assumed that population at onset outbreak. Through our analysis, derived invasion reproduction number an HIV-endemic setting established existence local asymptotic stability Mpox-free equilibrium under conditions. Furthermore, demonstrated Mpox-endemic regime. Notably, findings revealed exhibits backward bifurcation, phenomenon may not occurred absence population. significance results presence could hinder efforts Mpox, allowing disease become even when below one. Additionally, found might be more challenging scenarios where increases susceptibility Mpox. Finally, consistent previous studies, analysis confirms reducing contact can effective controlling community.</p>

Язык: Английский

Процитировано

4
Mpox in Pregnancy: Unraveling the Maternal-Fetal Risks of a Re-Emerging Disease, a narrative review DOI Creative Commons
Benedetta Rossi, Giorgio Tiecco,

Jacopo Logiudice

и другие.

Journal of Infection and Public Health, Год журнала: 2025, Номер 18(5), С. 102758 - 102758

Опубликована: Март 13, 2025

Язык: Английский

Процитировано

2
Development and Validation of LAMP Assays for Distinguishing MPXV Clades with Fluorescent and Colorimetric Readouts DOI Creative Commons
Nazente Atçeken, Sara Asghari Dilmani, Ahmed Abdullah

и другие.

Biosensors, Год журнала: 2025, Номер 15(1), С. 23 - 23

Опубликована: Янв. 6, 2025

Human monkeypox (Mpox) is a zoonotic disease caused by the Monkeypox virus (MPXV). As of 14 August 2024, World Health Organization (WHO) has declared it global health emergency. For Mpox, this was second public emergency significance in past two years. MPXV belongs to Poxviridae family and phylogenetically epidemically divided into clades: Congo Basin (Clade-I) West African (Clade-II) clades. Clade-I been associated with more severe progression higher mortality compared Clade-II, thus differentiation between clades can play an important role predicting prognosis. The LAMP technique advantages not requiring thermal cycling achieving amplification shorter time qPCR. Different types assays were developed study benefit from these advantages. We report development LAMP-1 LAMP-2 using method detect respectively. assay includes both fluorescence visible colorimetric readout tests sensitivities 103 107 copies, assay, probe-based test utilizing Novel R-Duplex DARQ probe developed, offering detection at sensitivity copies. result, we successfully three highly specific molecular diagnostic that distinctly differentiate clades, delivering essential tools for precise diagnosis effective control Mpox.

Язык: Английский

Процитировано

1
Monkeypox: a re-emergent virus with global health implications – a comprehensive review DOI Creative Commons
Nourhan G. Naga,

Enas A. Nawar,

A’laa A. Mobarak

и другие.

Tropical Diseases Travel Medicine and Vaccines, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 14, 2025

Abstract Monkeypox virus (MPXV) is an enclosed, double-stranded DNA from the Orthopoxvirus genus, which also contains variola, vaccinia, and cowpox. MPXV, was once confined to West Central Africa, has recently had a rebound, spreading beyond its original range since 2017. The distinguished by unique morphology, includes oval or brick-shaped structure complex lipid protein makeup. current multi-country outbreak designated public health emergency in 2022, highlighted MPXV’s shifting epidemiology ability spread rapidly over globe. ‘No one safe until everyone safe’ slogan we often heard during COVID-19 pandemic, now required for growing global regional mpox outbreaks. epidemic divided into two clades: Clade I II, have distinct pathogenic characteristics. Diagnostic approaches developed with advances molecular techniques, yet problems persist resource-constrained situations. This overview summarizes virus’s history, epidemiology, clinical characteristics, offering insights recent comeback response efforts.

Язык: Английский

Процитировано

1
Mpox Virus as a Global Public Health Emergency: A Scoping Review DOI Creative Commons
Sathish Sankar, Pachamuthu Balakrishnan, Yean K. Yong

и другие.

Canadian Journal of Infectious Diseases and Medical Microbiology, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

The monkeypox (Mpox) virus has emerged as a global public health emergency of international concern recently. that was endemic in West and Central Africa now been reported with chains transmission to several countries. A scoping review carried out from the relevant literature available PubMed, Scopus Web Science. This comprehensive analysis describes epidemiology, pathogenesis, clinical manifestations, complications including secondary bacterial infections, diagnosis, treatment vaccination. article underscores significance key viral immune mediators infection discusses updated recommendations on therapeutic strategies

Язык: Английский

Процитировано

1
The resurgence of monkeypox clade Ib: a global health emergency and concern DOI
Suresh Kumar, Gunasekaran Subramaniam,

K. J. Senthil Kumar

и другие.

Archives of Virology, Год журнала: 2025, Номер 170(4)

Опубликована: Март 21, 2025

Язык: Английский

Процитировано

1
A Review of the Molecular Understanding of the Mpox Virus (MPXV): Genomics, Immune Evasion, and Therapeutic Targets DOI Creative Commons
Edgar Cambaza

Zoonotic Diseases, Год журнала: 2025, Номер 5(1), С. 3 - 3

Опубликована: Янв. 16, 2025

The Mpox virus (MPXV), a zoonotic pathogen from the Orthopoxvirus genus, has emerged as significant global public health concern, especially after unprecedented outbreak in 2022. This review synthesizes MPXV’s molecular features, focusing on its genomic structure, replication mechanisms, immune evasion strategies, and implications for diagnostics therapeutics. study examines virus’s organization utilizing recent peer-reviewed literature, highlighting essential genes like OPG027 D1L, which contribute to host adaptation, increased transmissibility, evasion. Advances diagnostics, including real-time PCR genome sequencing, are reviewed, emphasizing their critical role monitoring control. However, challenges persist, such diagnostic limitations resource-constrained settings lack of targeted vaccines antivirals. discusses new antiviral candidates, confirmed through computational vitro techniques, identifying thymidine kinase VP39 key therapeutic targets. Emphasizing need surveillance track adaptive evolution, results show that particular mutations, D1L genes, increase transmissibility MPXV. These revelations highlight urgent necessity better catered towards addressing present constraints developing focused treatments reduce effect virus. emphasizes how these underscore combined plans handle changing MPXV epidemiology properly.

Язык: Английский

Процитировано

1
Mpox: Global epidemic situation and countermeasures DOI Creative Commons

Wen-Shuang Hou,

Nan Wu,

Yanzhi Liu

и другие.

Virulence, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 8, 2025

Mpox, is a zoonotic disease caused by the monkeypox virus and primarily endemic to Africa. As countries gradually stop smallpox vaccination, resistance declining, increasing risk of infection with mpox other viruses. On 14 August 2024, World Health Organization announced that spread constituted public health emergency international concern. Mpox's transmission routes symptoms are complex pose new challenges global health. Several vaccines (such as ACAM2000, JYNNEOS, LC16m8, genetically engineered vaccines) antiviral drugs tecovirimat, brincidofovir, cidofovir, varicella immunoglobulin intravenous injection) have been developed marketed prevent control this disease. This review aims introduce epidemic situation, epidemiological characteristics, physiological pathological preventive measures for in detail, provide scientific basis prevention viruses worldwide.

Язык: Английский

Процитировано

1