Toward Mycobacterium tuberculosis Virulence Inhibition: Beyond Cell Wall DOI Creative Commons
М. С. Котлярова, Mikhail S. Shumkov, А. В. Гончаренко

и другие.

Microorganisms, Год журнала: 2024, Номер 13(1), С. 21 - 21

Опубликована: Дек. 26, 2024

Mycobacterium tuberculosis (Mtb) is one of the most successful bacterial pathogens in human history. Even antibiotic era, Mtb widespread and causes millions new cases each year. The ability to disrupt host's innate adaptive immunity, as well natural persistence, complicates disease control. Tuberculosis traditional therapy involves long-term use several antibiotics. Treatment failures are often associated with development resistance or more drugs. medicines that act on targets will expand treatment options for caused by multidrug-resistant extensively drug-resistant Mtb. Therefore, drugs target virulence factors an attractive strategy. Such do not have a direct bacteriostatic bactericidal effect, but can disarm pathogen so host immune system becomes able eliminate it. Although cell wall-associated being actively studied anti-TB drug development, other important adaptation interaction also worth comprehensive analysis. In this review, specific (such secreted phosphatases, regulatory systems, ESX-1 secretion system) identified promising novel anti-virulence development. Additionally, models search inhibitors discussed, such virtual screening silico, vitro enzyme inhibition assay, recombinant strains reporter constructs, phenotypic analysis using infection environments.

Язык: Английский

VFDB 2025: an integrated resource for exploring anti-virulence compounds DOI Creative Commons
Siyu Zhou, Bo Liu, Dandan Zheng

и другие.

Nucleic Acids Research, Год журнала: 2024, Номер 53(D1), С. D871 - D877

Опубликована: Окт. 29, 2024

Abstract With the escalating crisis of bacterial multidrug resistance, anti-virulence therapeutic strategies have emerged as a highly promising alternative to conventional antibiotic treatments. Anti-virulence compounds are specifically designed target virulence factors (VFs), disarming pathogens without affecting growth and thus reduce selective pressure for resistance development. However, due complexity pathogenesis, no small molecules been approved clinical use far, despite documentation hundreds potential candidates. To provide valuable reference resources drug design, repurposing, selection, factor database (VFDB, http://www.mgc.ac.cn/VFs/) has systematically collected public data on through extensive literature mining, further integrated this information with its existing knowledge VFs. date, VFDB curated comprehensive dataset 902 across 17 superclasses reported by 262 studies worldwide. By cross-linking current VFs relevant (e.g. classification, chemical structure, molecular targets mechanisms action), aims bridge gap between chemists microbiologists, providing crucial insights development innovative effective antibacterial therapies combat infections address resistance.

Язык: Английский

Процитировано

18

Chalcone derivative enhance poultry meat preservation through quorum sensing inhibition against Salmonella (Salmonella enterica serovar Typhi) contamination DOI
S.P. Ramya Ranjan Nayak,

Pratik Pohokar,

Anamika Das

и другие.

Food Control, Год журнала: 2025, Номер unknown, С. 111155 - 111155

Опубликована: Янв. 1, 2025

Процитировано

0

Nanoenabling MbtI Inhibitors for Next-Generation Tuberculosis Therapy DOI Creative Commons
Giulia Cazzaniga, Matteo Mori, Anna Griego

и другие.

Journal of Medicinal Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

The urgent need for safer and innovative antitubercular agents remains a priority the scientific community. In pursuit of this goal, we designed evaluated novel 5-phenylfuran-2-carboxylic acid derivatives targeting Mycobacterium tuberculosis (Mtb) salicylate synthase (MbtI), key enzyme, absent in humans, that plays crucial role Mtb virulence. Several potent MbtI inhibitors demonstrating significant activity favorable safety profile were identified. Structure-guided optimization yielded 5-(3-cyano-5-isobutoxyphenyl)furan-2-carboxylic (1e), which exhibited strong inhibition (IC50 = 11.2 μM) promising vitro (MIC99 32 μM against M. bovis BCG). Esters 1e effectively loaded into poly(2-methacryloyloxyethyl phosphorylcholine)-poly(2-(diisopropylamino)ethyl methacrylate) (PMPC-PDPA) polymersomes (POs) delivered to intracellular mycobacteria, resulting reduced viability. This study provides foundation use POs development future MbtI-targeted therapies tuberculosis.

Язык: Английский

Процитировано

0

Functionalization of cationic porphyrins with peripheral platinum(II) complexes to optimize photodynamic therapy against Candida- associated infections: a focus on denture stomatitis and burn wounds DOI Creative Commons
Maíra Terra Garcia, Lara Luise Castro Pedroso, Paulo Henrique Fonseca do Carmo

и другие.

Mycology&#58 An International Journal on Fungal Biology, Год журнала: 2025, Номер unknown, С. 1 - 20

Опубликована: Март 11, 2025

Язык: Английский

Процитировано

0

Therapeutic effect and concomitant toxicity of Hydrargyrum Chloratum Compositum on chronic difficult-to-heal wounds in rats DOI
Yang Yu, Ping Huang, Jingjing Yang

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119749 - 119749

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

New Methodologies as Opportunities in the Study of Bacterial Biofilms, Including Food-Related Applications DOI Creative Commons
Francesca Coppola, Florinda Fratianni, Vittorio Bianco

и другие.

Microorganisms, Год журнала: 2025, Номер 13(5), С. 1062 - 1062

Опубликована: Май 2, 2025

Traditional food technologies, while essential, often face limitations in sensitivity, real-time detection, and adaptability to complex biological systems such as microbial biofilms. These constraints have created a growing demand for more advanced, precise, non-invasive tools ensure safety quality. In response these challenges, cross-disciplinary technological integration has opened new opportunities the industry public health, leveraging methods originally developed other scientific fields. Although their industrial-scale implementation is still evolving, application research pilot settings already significantly improved our ability detect control biofilms, thereby strengthening protocols. Advanced analytical techniques, identification of pathogenic species virulence markers, screening “natural” antimicrobial compounds can now be conceptualized interconnected elements within virtual framework centered on “food” “biofilm”. this short review, starting from basic concepts biofilm associated microorganisms, we highlight selection emerging approaches—from optical methods, microfluidics, Atomic Force Microscopy (AFM), biospeckle techniques molecular strategies like CRISPR, qPCR, NGS, use organoids. Initially conceived biomedical biotechnological applications, recently demonstrated value science by enhancing understanding behavior supporting discovery novel anti-biofilm strategies.

Язык: Английский

Процитировано

0

A multi-computational and crystallographic investigation for the antibiotic mechanism of two 2,4-bis(4-methoxyphenamino)pyrimidines DOI
Goutam Biswas, Mohd Afzal, Umesh Chandra Halder

и другие.

Journal of Molecular Structure, Год журнала: 2024, Номер unknown, С. 140358 - 140358

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

2

Toward Mycobacterium tuberculosis Virulence Inhibition: Beyond Cell Wall DOI Creative Commons
М. С. Котлярова, Mikhail S. Shumkov, А. В. Гончаренко

и другие.

Microorganisms, Год журнала: 2024, Номер 13(1), С. 21 - 21

Опубликована: Дек. 26, 2024

Mycobacterium tuberculosis (Mtb) is one of the most successful bacterial pathogens in human history. Even antibiotic era, Mtb widespread and causes millions new cases each year. The ability to disrupt host's innate adaptive immunity, as well natural persistence, complicates disease control. Tuberculosis traditional therapy involves long-term use several antibiotics. Treatment failures are often associated with development resistance or more drugs. medicines that act on targets will expand treatment options for caused by multidrug-resistant extensively drug-resistant Mtb. Therefore, drugs target virulence factors an attractive strategy. Such do not have a direct bacteriostatic bactericidal effect, but can disarm pathogen so host immune system becomes able eliminate it. Although cell wall-associated being actively studied anti-TB drug development, other important adaptation interaction also worth comprehensive analysis. In this review, specific (such secreted phosphatases, regulatory systems, ESX-1 secretion system) identified promising novel anti-virulence development. Additionally, models search inhibitors discussed, such virtual screening silico, vitro enzyme inhibition assay, recombinant strains reporter constructs, phenotypic analysis using infection environments.

Язык: Английский

Процитировано

0