Temperature sensing and virulence regulation in pathogenic bacteria

Trends in Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Pathogenic bacteria can detect a variety of environmental signals, including temperature changes. While sudden and significant variations act as danger signals that trigger protective heat-shock response, minor fluctuations typically signal to the pathogen it has moved from one environment another, such entering specific niche within host during infection. These latter are utilized by pathogens coordinate expression crucial virulence factors. Here, we elucidate critical role in governing factors bacterial pathogens. Moreover, outline molecular mechanisms used fluctuations, focusing on systems employ proteins nucleic acids sensory devices. We also discuss potential implications extent risk climate change poses human pathogenic diseases.

Язык: Английский

Detection and co-occurrence of Acanthamoeba and Klebsiella pneumoniae in freshwater river systems of Taichung, Taiwan

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 15, 2025

This study focuses on the detection and co-occurrence of Acanthamoeba Klebsiella pneumoniae in freshwater river systems. Both microorganisms are known for their pathogenic potential, with capable causing infections such as amoebic keratitis, K. being a common cause community infections. Understanding presence distribution natural aquatic environments can provide insights into potential public health risks, especially regions significant human-water interactions. Water samples were collected from five major hydrophilic Taichung, Taiwan. Polymerase chain reaction assays employed to detect pneumoniae. In addition microbial detection, environmental parameters, including temperature, pH, reactive oxygen species, water hardness, measured assess influence presence. Data analysis focused identifying patterns between two microorganisms. The revealed variable rates both across sampling sites. Co-occurrence was observed at several locations, indicating interaction environment. While quality parameters measured, no strong correlation found these factors rates. widespread organisms highlights resilience diverse environments. However, this did not include virulence factor analysis, role conditions modulating bacterial pathogenicity remains speculative. research demonstrates that prevalent ecosystems Taiwan, notable instances co-occurrence. Although temperature pH significantly rates, frequently accessed bodies may pose risks. Further is necessary explore ecological interactions impact human health, particularly high activity near sources.

Язык: Английский

Global quantitative proteome analysis of a multi-resistant Klebsiella pneumoniae strain

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 19, 2025

Klebsiella pneumoniae is a critical nosocomial pathogen with rising incidence in antibiotic resistance worldwide. Multidrug-resistant strains of K. pose major health threat, particularly to immunosuppressed and elderly patients. To date, no effective vaccine formulations have been developed. Therefore, the creation novel therapeutic preventive treatments key importance. Proteins play central role host-pathogen interactions, identifying characterizing conserved, organism-specific proteins essential for formulations. Capsule-deficient ATCC BAA-2146Δwza was cultured minimal medium exposed two physiological stress factors profile its cellular proteome exoproteome adaptation infection mimicking conditions by mass spectrometry. More than 2,800 covering 54% entire annotated proteome, were profiled untreated controls after imposition heat or oxidative stress. The proteomic data revealed that some outer membrane (OMPs), including LPP other virulence involved iron acquisition cell wall remodeling, more abundant proteome. Approximately one-third assigned OMPs lipoproteins, i.e., represent important fitness might be immunogenic due their structure position bacterial membrane. In particular, lipoproteins Pal SlyB showed highest abundance among OM intracellular all tested conditions. These findings suggest these may promising candidates further immunoproteomic analyses assess immunogenicity pathogenicity.

Язык: Английский