Lipopolysaccharide induces retention of E-cadherin in the endoplasmic reticulum and promotes hybrid epithelial-to-mesenchymal transition of human embryonic stem cells-derived expandable lung epithelial cells

Inflammation Research, Год журнала: 2025, Номер 74(1)

Опубликована: Май 24, 2025

Abstract Background Lipopolysaccharide (LPS)-induced inflammation of lung tissues triggers irreversible alterations in the parenchyma, leading to fibrosis and pulmonary dysfunction. While molecular cellular responses immune connective tissue cells lungs are well characterized, specific epithelial response remains unclear due lack representative cell models. Recently, we introduced human embryonic stem cell-derived expandable (ELEP) as a novel model for studying injury regeneration. Methods ELEPs were derived from CCTL 14 line through activin A-mediated endoderm specification, followed by further induction toward epithelium using FGF2 EGF. exhibit high proliferation rate express key structural markers alveolar progenitors, such NKX2-1. The effects Escherichia coli LPS serotype O55:B5 on phenotype signaling analyzed viability migration assays, mRNA protein levels determined qRT-PCR, western blotting, immunofluorescent microscopy. Results We demonstrated that purified induces features hybrid epithelial-to-mesenchymal transition pluripotent ELEPs, unfolded response, upregulates intracellular β-catenin level retention E-cadherin within endoplasmic reticulum. Conclusions Human provide biologically relevant, non-cancerous investigate inflammatory stimuli address plasticity. This approach offers insights into fine processes underlying repair.

Язык: Английский

Bioactivity and antibiofilm activity of metal and non-metal nanoparticles in DES against resistant microorganisms

International Biodeterioration & Biodegradation, Год журнала: 2025, Номер 203, С. 106121 - 106121

Опубликована: Май 27, 2025

Язык: Английский