Metabolic Reprogramming in Gut Microbiota Exposed to Polystyrene Microplastics

Biomedicines, Год журнала: 2025, Номер 13(2), С. 446 - 446

Опубликована: Фев. 12, 2025

Background: Microplastics (MPs) are small plastic fragments with diameters less than 5 mm in size and prevalent everyday essentials consumables. Large global production has now led to a flooding of MPs our natural environment. Due their detrimental impacts on the planet's ecosystems potentially health, have emerged as significant public health concern. In this pilot study, we hypothesize that exposure will negatively affect gut microbiota composition function, which metabolic reprogramming plays an important role. Methods: Using vitro experiments, three bacterial strains (Escherichia coli MG1655, Nissle 1917, Lactobacillus rhamnosus) were selected investigate exposure. The individually cultured anaerobic chamber exposed 1 µm polystyrene at various concentrations (0, 10, 20, 50, 100, 500 µg/mL) culture medium. Results: reduced growth all dose-dependent manner. Liquid chromatography mass spectrometry (LC-MS)-based untargeted metabolomics revealed differences multiple pathways, such sulfur metabolism amino sugar nucleotide metabolism. addition, extracted from C57BL/6 mice, 16S rRNA sequencing results showed upregulation Lactobacillales reduction Erysipelotrichales due Furthermore, targeted corroborated alterations microbial tryptophan energy producing glycolysis/gluconeogenesis pentose phosphate pathway. Conclusions: These findings provide evidence causes comprehensive changes healthy microbiota, may also insights into mechanistic effects humans.

Язык: Английский

Microplastics in freshwater ecosystems: sources, research methods and environmental consequences. Experience of russian researchers and prospects for pollution control in khanty-mansiysk autonomous okrug – yugra

Bulletin of Nizhnevartovsk State University, Год журнала: 2025, Номер 1, С. 111 - 135

Опубликована: Март 22, 2025

This article presents an analytical review of scientific studies focusing on the issue microplastic pollution in freshwater bodies Russia. The study examines detail primary sources particles entering aquatic environments, including domestic and industrial wastewater, rainwater runoff, urban dust, diffuse such as decomposition plastic waste landfills natural environments. Special attention is given to methods collecting water sediment samples employed by various research groups. Contemporary approaches detection identification are described, visual using optical electron microscopy, Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy. data impact microplastics biological organisms ecosystems, disruptions trophic networks impairments functioning endocrine, reproductive, immune systems. Aspects mechanical toxicological effects considered, well processes contaminant sorption onto their surfaces. conclusion highlights need for further standardization methodologies additional investigations gain a deeper understanding extent distribution particularly regions with insufficient empirical data. importance developing comprehensive strategies minimize freshwaters emphasized.

Язык: Английский