Homogeneity Between Cervical and Vaginal Microbiomes and the Diagnostic Limitations of 16S Sequencing for STI Pathogens at Higher Ct Values DOI Open Access
Claudio Neidhöfer, Mateja Condic,

Nynke E. Hahn

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1983 - 1983

Опубликована: Фев. 25, 2025

Understanding the interactions between cervico-vaginal microbiome, immune responses, and sexually transmitted infections (STIs) is crucial for developing targeted diagnostic therapeutic strategies. Although microbiome analyses are not yet standard practice, integrating them into routine diagnostics could enhance personalized medicine therapies. We investigated extent to which partial 16S short-read amplicon inform on presence of six commonly encountered STI-causing pathogens in a patient cohort referred colposcopy, whether relevant taxonomic or discrepancies occur when using vaginal rather than cervical swabs. The study included samples collected from women colposcopy at University Hospital Bonn November 2021 February 2022, due an abnormal PAP smear positive hrHPV results. rRNA gene sequencing libraries were prepared targeting V1-V2 V4 regions RNA sequenced Illumina MiSeq. PCR common conducted Allplex STI Essential Assay Kit (Seegene, Seoul, Republic Korea). Concerning bacterial no significant differences found terms prevalence taxa present diversity. A total 95 patients 171 tested least one among Ureaplasma parvum, urealyticum, Mycoplasma hominis, genitalium, Chlamydophila trachomatis Neisseria gonorrhoeae. Sequencing region enabled detection one-third half PCR-positive samples, with likelihood increasing lower cycle threshold (Ct) values. In contrast, was less effective overall, yielding fewer species-level identifications higher proportion undetermined taxa. demonstrate that closely mirrors relationship has been explored previously, but broadens possibilities analysis pathogen establishes swabs as reliable method detecting pathogens, sensitivities comparable superior endocervical On other hand, sensitivity appears insufficient diagnostics, it fails reliably identify even detect Ct

Язык: Английский

Homogeneity Between Cervical and Vaginal Microbiomes and the Diagnostic Limitations of 16S Sequencing for STI Pathogens at Higher Ct Values DOI Open Access
Claudio Neidhöfer, Mateja Condic,

Nynke E. Hahn

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1983 - 1983

Опубликована: Фев. 25, 2025

Understanding the interactions between cervico-vaginal microbiome, immune responses, and sexually transmitted infections (STIs) is crucial for developing targeted diagnostic therapeutic strategies. Although microbiome analyses are not yet standard practice, integrating them into routine diagnostics could enhance personalized medicine therapies. We investigated extent to which partial 16S short-read amplicon inform on presence of six commonly encountered STI-causing pathogens in a patient cohort referred colposcopy, whether relevant taxonomic or discrepancies occur when using vaginal rather than cervical swabs. The study included samples collected from women colposcopy at University Hospital Bonn November 2021 February 2022, due an abnormal PAP smear positive hrHPV results. rRNA gene sequencing libraries were prepared targeting V1-V2 V4 regions RNA sequenced Illumina MiSeq. PCR common conducted Allplex STI Essential Assay Kit (Seegene, Seoul, Republic Korea). Concerning bacterial no significant differences found terms prevalence taxa present diversity. A total 95 patients 171 tested least one among Ureaplasma parvum, urealyticum, Mycoplasma hominis, genitalium, Chlamydophila trachomatis Neisseria gonorrhoeae. Sequencing region enabled detection one-third half PCR-positive samples, with likelihood increasing lower cycle threshold (Ct) values. In contrast, was less effective overall, yielding fewer species-level identifications higher proportion undetermined taxa. demonstrate that closely mirrors relationship has been explored previously, but broadens possibilities analysis pathogen establishes swabs as reliable method detecting pathogens, sensitivities comparable superior endocervical On other hand, sensitivity appears insufficient diagnostics, it fails reliably identify even detect Ct

Язык: Английский

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