Liver kinase B1 (LKB1) regulates the epigenetic landscape of mouse pancreatic beta cells DOI Creative Commons
Nejc Haberman, Rebecca Cheung,

Grazia Pizza

и другие.

The FASEB Journal, Год журнала: 2024, Номер 38(16)

Опубликована: Авг. 14, 2024

Liver kinase B1 (LKB1/STK11) is an important regulator of pancreatic β-cell identity and function. Elimination Lkb1 from the results in improved glucose-stimulated insulin secretion accompanied by profound changes gene expression, including upregulation several neuronal genes. The mechanisms through which LKB1 controls expression are, at present, poorly understood. Here, we explore impact β cell-selective deletion on chromatin accessibility mouse islets. To characterize role regulation transcriptional level, combine these data with a map islet active transcription start sites histone marks. We demonstrate that elimination β-cells widespread accessibility, correlating transcript levels. Changes occurred hundreds promoter enhancer regions, many were close to reveal dysregulated enhancers are enriched binding motifs for factors (TFs) identity, such as FOXA, MAFA or RFX6, identify microRNAs (miRNAs) regulated level. Overall, our study provides new insights into epigenetic regulates

Язык: Английский

Maturation of beta cells: lessons from in vivo and in vitro models DOI Creative Commons
Tom Barsby, Timo Otonkoski

Diabetologia, Год журнала: 2022, Номер 65(6), С. 917 - 930

Опубликована: Март 4, 2022

Abstract The ability to maintain normoglycaemia, through glucose-sensitive insulin release, is a key aspect of postnatal beta cell function. However, terminally differentiated identity does not necessarily imply functional maturity. Beta maturation therefore continuation development, albeit process that occurs postnatally in mammals. Although many important features have been identified the study maturation, as yet no unified mechanistic model maturity exists. Here, we review recent findings about underlying mechanisms maturation. These include systemic hormonal and nutritional triggers operate energy-sensing machinery shifts within cells, resulting primed metabolic states allow for appropriate glucose trafficking and, ultimately, release. We also draw attention expansive synergistic nature these pathways emphasise dependent on overlapping regulatory networks. Graphical abstract

Язык: Английский

Процитировано

37

miR-92a-3p and miR-182-3p as potential biomarkers for the differential diagnosis of gestational diabetes mellitus and its correlation with pregnancy outcomes DOI
Xin Du, Qi Li,

Zhenlan Wu

и другие.

Irish Journal of Medical Science (1971 -), Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Язык: Английский

Процитировано

0

Mechanisms Underlying the Expansion and Functional Maturation of β-Cells in Newborns: Impact of the Nutritional Environment DOI Open Access
Cécile Jacovetti, Romano Regazzi

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(4), С. 2096 - 2096

Опубликована: Фев. 14, 2022

The functional maturation of insulin-secreting β-cells is initiated before birth and completed in early postnatal life. This process has a critical impact on the acquisition an adequate β-cell mass capacity to meet adapt insulin needs later Many cellular pathways playing role development have already been identified. However, single-cell transcriptomic proteomic analyses continue reveal new players contributing identity. In this review, we provide updated picture mechanisms governing expansion transition cells from immature mature state. We then highlight contribution environment discuss adverse utero neonatal characterized by calorie fat overload or protein deficiency undernutrition. Inappropriate nutrition life constitutes risk factor for developing diabetes adulthood can affect offspring over two generations. A better understanding these events occurring period will help strategies produce design novel therapeutic approaches prevention treatment diabetes.

Язык: Английский

Процитировано

10

Effects of Phytochemicals on Type 2 Diabetes via MicroRNAs DOI Creative Commons
Dilek Sivri, Makbule Gezmen Karadağ

Current Nutrition Reports, Год журнала: 2024, Номер 13(3), С. 444 - 454

Опубликована: Май 28, 2024

Abstract Purpose of Review Type 2 diabetes, characterized by inadequate insulin secretion and resistance, is increasingly prevalent. To effectively manage type identifying new therapeutic targets crucial. MicroRNAs, short noncoding RNA molecules, play a pivotal role in regulating β-cell function, production, show promise as biomarkers for predicting diabetes onset. Phytochemicals, known their antioxidant activities, may influence microRNA expression, potentially improving sensitivity mitigating associated complications. This review aims to explore the significance potential biomarkers, how certain phytochemicals modulate expressions reduce or prevent its Recent Findings Current research suggests that microRNAs novel diagnosing monitoring diabetic Additionally, regulate control presenting strategy. Summary The multifactorial effects on complications through warrant further elucidate mechanisms. Comprehensive clinical trials are needed assess safety efficacy combinations. Given ability incorporating phytochemical-rich foods into diet be beneficial.

Язык: Английский

Процитировано

1

Unraveling diabetes complexity through natural products, miRNAs modulation, and future paradigms in precision medicine and global health DOI
Fahrul Nurkolis, Elvan Wiyarta, Nurpudji Astuti Taslim

и другие.

Clinical Nutrition ESPEN, Год журнала: 2024, Номер 63, С. 283 - 293

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

0

Liver kinase B1 (LKB1) regulates the epigenetic landscape of mouse pancreatic beta cells DOI Open Access
Nejc Haberman,

Rebecca Cheung,

Grazia Pizza

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 15, 2024

Liver kinase B1 (LKB1/STK11) is an important regulator of pancreatic β-cell identity and function. Elimination

Язык: Английский

Процитировано

0

Liver kinase B1 (LKB1) regulates the epigenetic landscape of mouse pancreatic beta cells DOI Creative Commons
Nejc Haberman, Rebecca Cheung,

Grazia Pizza

и другие.

The FASEB Journal, Год журнала: 2024, Номер 38(16)

Опубликована: Авг. 14, 2024

Liver kinase B1 (LKB1/STK11) is an important regulator of pancreatic β-cell identity and function. Elimination Lkb1 from the results in improved glucose-stimulated insulin secretion accompanied by profound changes gene expression, including upregulation several neuronal genes. The mechanisms through which LKB1 controls expression are, at present, poorly understood. Here, we explore impact β cell-selective deletion on chromatin accessibility mouse islets. To characterize role regulation transcriptional level, combine these data with a map islet active transcription start sites histone marks. We demonstrate that elimination β-cells widespread accessibility, correlating transcript levels. Changes occurred hundreds promoter enhancer regions, many were close to reveal dysregulated enhancers are enriched binding motifs for factors (TFs) identity, such as FOXA, MAFA or RFX6, identify microRNAs (miRNAs) regulated level. Overall, our study provides new insights into epigenetic regulates

Язык: Английский

Процитировано

0