Skin Disorders and Osteoporosis: Unraveling the Interplay Between Vitamin D, Microbiota, and Epigenetics Within the Skin–Bone Axis DOI Open Access

Vincenzo Papa,

Federica Li Pomi, Paola Lucia Minciullo

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 179 - 179

Опубликована: Дек. 28, 2024

Growing scientific evidence suggests a strong interconnection between inflammatory skin diseases and osteoporosis (OP), systemic condition characterized by decreased bone density structural fragility. These conditions seem to share common pathophysiological mechanisms, including immune dysregulation, chronic inflammation, vitamin D deficiency, which play crucial role in both health. Additionally, the roles of gut microbiota (GM) epigenetic regulation via microRNAs (miRNAs) emerge as key elements influencing progression conditions. This review aims examine skin–bone axis, exploring how factors such D, GM, miRNAs interact subtle interplay driving inflammation immune-metabolic alterations. Recent research that combined therapeutic approaches—including supplementation, targeted interventions, miRNA-based therapies—could be promising strategies for managing comorbid OP. perspective highlights need multidisciplinary approaches clinical management related skin-bone axis.

Язык: Английский

MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression DOI Creative Commons
Yingchao Shen, Yang Zhang, Qiang Wang

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2024, Номер 19(1)

Опубликована: Фев. 12, 2024

Abstract Stimulating bone formation potentially suggests therapeutics for orthopedic diseases including osteoporosis and osteoarthritis. Osteoblasts are key to remodeling because they act as the only bone-forming cells. miR-877-5p has a chondrocyte-improving function in osteoarthritis, but its effect on osteoblast differentiation is unknown. Here, miR-877-5p-mediated was studied. Real-time reverse transcriptase-polymerase chain reaction performed measure expression during osteogenic of MC3T3-E1 Osteoblast markers, alkaline phosphatase (ALP), collagen type I a1 chain, osteopontin, were measured detected by alizarin red staining ALP staining. Potential targets predicted from three different algorithms: starBase ( http://starbase.sysu.edu.cn/ ), PITA http://genie.weizmann.ac.il/pubs/mir07/mir07_data.html miRanda http://www.microrna.org/microrna/home.do ). It further verified dual luciferase reporter gene assay. The experimental results found that upregulated Overexpression promoted differentiation, which characterized increased cell mineralization, activity, osteogenesis-related expression. Knockdown produced opposite result. Dual assay showed directly targeted eukaryotic translation initiation factor 4γ2 (EIF4G2). EIF4G2 inhibited reversed promoting overexpression miR-135-5p differentiation. These indicate might have therapeutic application related promotion through targeting EIF4G2.

Язык: Английский

Процитировано

6
RUNX2 regulation in osteoblast differentiation: A possible therapeutic function of the lncRNA and miRNA-mediated network DOI

Pakkath Narayanan Arya,

I. Saranya,

N. Selvamurugan

и другие.

Differentiation, Год журнала: 2024, Номер 140, С. 100803 - 100803

Опубликована: Июль 23, 2024

Язык: Английский

Процитировано

4
Mir-381-3p aggravates ovariectomy-induced osteoporosis by inhibiting osteogenic differentiation through targeting KLF5/Wnt/β-catenin signaling pathway DOI Creative Commons

Yingwei Zhao,

Jingsong Liu, Yubo Zhang

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2024, Номер 19(1)

Опубликована: Авг. 17, 2024

Increasing evidence shows the pivotal significance of miRNAs in pathogenesis osteoporosis. miR-381-3p has been identified as an inhibitor osteogenesis. This study explored role and mechanism postmenopausal osteoporosis (PMOP), most common type Bilateral ovariectomy (OVX) rat model was established antagomir administrated through tail vein vivo. The pathological changes rats were assessed evaluation serum bone turnover markers (BALP, PINP, CTX-1), hematoxylin eosin (H&E) staining, well expression osteoblast differentiation biomarkers. Moreover, isolated marrow mesenchymal stem cells from OVX-induced (OVX-BMMSCs) utilized to explore impact on differentiation. In addition, target gene downstream pathway further investigated both vivo vitro. elevated, whereas KLF5 suppressed OVX rats. decreased levels markers, improved trabecular separation, promoted biomarker ALP activity mineralization suppressed, biomarkers impeded after overexpression during OVX-BMMSCs. While contrasting results found inhibition miR-381-3p. targets KLF5, negatively affecting its Wnt/β-catenin pathway, Silencing restored activation induced by antagomir. aggravates PMOP inhibiting osteogenic targeting KLF5/Wnt/β-catenin pathway. appears be a promising candidate for therapeutic intervention PMOP.

Язык: Английский

Процитировано

3
MicroRNAs and their Modulatory Effect on the Hallmarks of Osteosarcopenia DOI
William J. Silva, André Cruz, Gustavo Duque

и другие.

Current Osteoporosis Reports, Год журнала: 2024, Номер 22(5), С. 458 - 470

Опубликована: Авг. 20, 2024

Язык: Английский

Процитировано

3
Recent advances in the regulatory and non-coding RNA biology of osteogenic differentiation: biological functions and significance for bone healing DOI Creative Commons

Samaris Aranguren,

Heather Cole,

Lauren Jeannette Dargan

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 12

Опубликована: Янв. 6, 2025

Injuries associated with contemporary life, such as automobile crashes and sports injuries, can lead to large numbers of traumatic neuromuscular injuries that are intimately bone fractures. Regulatory non-coding RNAs play essential roles in multiple cellular processes, including osteogenic differentiation healing. In this review, we discuss the most recent advances our understanding regulatory RNA biology stem, stromal progenitor cells. We focused on circular RNAs, small nucleolar PIWI-interacting comprehensively summarized their biological functions well discussed significance for healing tissue regeneration.

Язык: Английский

Процитировано

0
Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection DOI Creative Commons

Shaqiu Zhang,

Jinku Li,

Mingshu Wang

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 5, 2025

Avian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis infected ducks. Since 2017, the morbidity mortality rates associated with ARV infection poultry have been on rise, progressively emerging significant viral disease impacting duck farming industry China. In our study, we collected embryo fibroblasts 18 h post-infection conducted transcriptome sequencing analysis. The analysis revealed that 3,818 mRNA expressions were up-regulated, 4,573 down-regulated, 472 long noncoding RNAs (LncRNAs) 345 lncRNAs down-regulated. We employed qRT-PCR validate results, confirming their accuracy. data indicated upregulation of PARP9 , TLR7 TRIM33 ATG5 genes, suggesting potential involvement infection. Notably, study identified novel functional lncRNA, MSTRG.9284.1 (It was named linc000889 present study), which inhibits replication at transcriptional, translational levels titer. Overall, this has numerous ARV-induced differentially expressed mRNAs lncRNAs, including lncRNA replication. This discovery provides new insights into mechanisms may contribute development prevention treatment strategies.

Язык: Английский

Процитировано

0
IGF2BP3 facilitates the osteogenic differentiation of bone marrow mesenchyml stem cells via upregulating KLK4 DOI

Jiazhu Tang,

Guoyang Zhao,

Zhao Jian-zhong

и другие.

Connective Tissue Research, Год журнала: 2025, Номер 66(1), С. 49 - 58

Опубликована: Янв. 2, 2025

Osteoporosis (OP) is a chronic metabolic bone disease marked by imbalance in osteoblast and osteoclast activity. This study was aimed to explore the molecular mechanism underlying osteogenic differentiation of marrow mesenchymal stem cells (BMSCs) discover novel target for OP. RT-qPCR used mRNA expression detection Kallikrein 4 (KLK4) Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3). Protein conducted western blot. The BMSCs evaluated through alkaline phosphatase (ALP) staining Alizarin Red (ARS). Interaction between IGF2BP3 KLK4 analyzed using RNA immunoprecipitation (RIP) assay actinomycin D assay. downregulated OP patients, upregulated osteogenically differentiated BMSCs. overexpression promoted enhanced KLK4. upregulation restored effect knockdown on Moreover, promoting These evidences suggested that contributed via mediating KLK4, providing potential treatment

Язык: Английский

Процитировано

0
Long Non-Coding RNAs in Stem Cell Regulation and Regenerative Medicine: Stemness, Differentiation, and Therapeutic Innovation DOI Creative Commons

Melika Emarati,

Hossein Azizi, Nima Ghasemi

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Long non-coding RNAs (lncRNAs) have emerged as critical regulators in stem cell biology, influencing cellular functions such pluripotency, differentiation, and self-renewal. Their unique ability to modulate gene expression at multiple levels—epigenetic, transcriptional, post-transcriptional—makes lncRNAs powerful tools for controlling fate. In regenerative medicine, understanding the roles of specific can enhance therapeutic approaches, particularly cell-based tissue repair engineering. By modulating lncRNA activity, researchers potentially direct differentiation toward desired lineages, facilitating development functional tissues clinical applications. This chapter explores how influence states, highlights current research therapies, discusses potential future applications where lncRNA-based interventions could drive advancements engineering medicine.

Язык: Английский

Процитировано

0
MIR155HG suppresses the osteogenic differentiation of bone marrow mesenchymal stem cells through regulating miR-155-5p and DKK1 expression DOI Creative Commons
Weimin Li, Cheng‐Han Yang,

Junji Xu

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 19, 2025

Increasing evidence has demonstrated that non-coding RNAs, including the lncRNA MIR155HG, are involved in pathogenesis of postmenopausal osteoporosis (PMOP). In current study, we studied MIR155HG function regulation osteogenic differentiation and tried to reveal underlying mechanisms. Forty blood samples taken from 20 PMOP patients (PMOP group) individuals without (control were used compare contents miR-155-5p via RT-PCR. Alizarin red S staining ALP evaluate potential bone marrow mesenchymal stem cells (BMSCs). Elevated levels observed group. Upregulation resulted decreased expression OPN, OSX, ALP, RUNX2 β-catenin but increased DKK1 expression, together with + areas. However, downregulation did not obviously change above indices induced by upregulation. Further experiments revealed caused an increase miR-155-5p, which also serves as inhibitor BMSCs through binding β-catenin. Consistent knockdown, only reverse repressive effect on osteoblastic differentiation, synchronously restored ability suppressed overexpression. regulating expression. Either inhibition or direct suppression may be effective approaches for treating PMOP.

Язык: Английский

Процитировано

0
LncRNA CRNDE ameliorates bone fracture by regulating cell viability and apoptosis of osteoblasts DOI Creative Commons
Yuanfeng Li, Shiqi Ye, Zhen Han

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Май 27, 2025

Delayed healing is a common postoperative complication among fractured patients, imposing an additional financial burden. This research examined the clinical relationship between CRNDE and delayed fracture (DFH) potential regulatory mechanisms underlying improvement. qRT-PCR was utilized to assess expression of miR-29a-3p in serum cellular samples, evaluate genes associated with osteogenic differentiation. The diagnostic predictive significance analyzed using ROC analysis logistic regression. Additionally, hFOB 1.19 differentiation model established. CCK-8 assay flow cytometry techniques were used investigate effects silencing CRNDE, as well concurrent inhibition both miR-29a-3p, on proliferation apoptosis cells. down-regulated, while up-regulated DFH patients. could effectively identify patients predict occurrence. In model, led significant decrease markers, reduction activity cells, increase apoptosis. There negative interaction miR-29a-3p. Concurrently inhibiting restore functional Serum holds biomarker for diagnosis prediction DFH. sponging effect ameliorate healing.

Язык: Английский

Процитировано

0