Antioxidants,
Год журнала:
2024,
Номер
13(12), С. 1500 - 1500
Опубликована: Дек. 9, 2024
Obesity
has
emerged
as
a
major
risk
factor
for
human
health,
exacerbated
by
aging
and
changes
in
dietary
habits.
It
represents
significant
health
challenge,
particularly
older
people.
While
numerous
studies
have
examined
the
effects
of
obesity
on
fat
metabolism
independently,
research
their
combined
is
limited.
In
present
study,
protective
action
against
white
accumulation
after
high-fat
diet
(HFD)
exerted
exogenous
melatonin,
circadian
hormone
endowed
with
antioxidant
properties
also
involved
metabolism,
was
investigated
mouse
model.
For
this
purpose,
battery
tests
applied
before
melatonin
treatments
animals,
including
epididymal
adipose
tissue
(eWAT)
histological
evaluations,
transcriptomic
lipidomic
analyses,
real-time
PCR
tests,
immunofluorescence
staining,
Western
blot,
appraisal
serum
levels,
transmission
electron
microscopy.
This
study
found
that
aged
mice
showed
increased
lipid
deposition,
inflammation,
reduced
glutathione
(GSH)
levels
compared
to
younger
mice.
Lipidomic
analyses
revealed
elevated
triglycerides,
diglycerides,
ceramides,
cholesterol,
along
decreased
sphingomyelin
fatty
acids
eWAT.
The
genes
linked
NF-κB
signaling,
autophagy,
pathways,
were
significantly
altered.
HFD
exhibited
Melatonin
supplementation
GSH
upregulated
AANAT
MTNR1A
expression
eWAT,
suggesting
alleviates
eWAT
damage
via
pathway.
suppressed
inflammatory
markers
(e.g.,
TNF-α,
NLRP3,
NF-κB,
IL-1β,
CEBPB)
preserved
mitochondrial
function
through
enhanced
mitophagy.
highlights
how
affect
gene
expression,
offering
potential
intervention
strategies.
These
findings
provide
important
insights
into
mechanisms
deposition
associated
diet,
Ageing Research Reviews,
Год журнала:
2024,
Номер
101, С. 102480 - 102480
Опубликована: Сен. 3, 2024
Mitochondria
functionally
degrade
as
neurons
age.
Degenerative
changes
cause
inefficient
oxidative
phosphorylation
(OXPHOS)
and
elevated
electron
leakage
from
the
transport
chain
(ETC)
promoting
increased
intramitochondrial
generation
of
damaging
reactive
oxygen
nitrogen
species
(ROS
RNS).
The
associated
progressive
accumulation
molecular
damage
causes
an
increasingly
rapid
decline
in
mitochondrial
physiology
contributing
to
aging.
Melatonin,
a
multifunctional
free
radical
scavenger
indirect
antioxidant,
is
synthesized
matrix
neurons.
Melatonin
reduces
ETC
elevates
ATP
production;
it
also
detoxifies
ROS/RNS
via
SIRT3/FOXO
pathway
upregulates
activities
superoxide
dismutase
2
glutathione
peroxidase.
influences
glucose
processing
by
In
neurogenerative
diseases,
often
adopt
Warburg-type
metabolism
which
excludes
pyruvate
mitochondria
causing
reduced
acetyl
coenzyme
A
production.
Acetyl
supports
citric
acid
cycle
OXPHOS.
Additionally,
required
co-substrate
for
arylalkylamine-N-acetyl
transferase,
rate
limits
melatonin
synthesis;
therefore,
production
diminished
cells
that
experience
making
more
vulnerable
stress.
Moreover,
endogenously
produced
diminishes
during
aging,
further
increasing
components.
More
normal
preserved
aging
with
supplementation.
Journal of Cellular Biochemistry,
Год журнала:
2025,
Номер
126(3)
Опубликована: Март 1, 2025
ABSTRACT
Breast
cancer
is
one
of
the
most
common
types
in
women,
and
metastasis
a
leading
cause
mortality
patients
with
this
disease.
This
study
investigated
effects
melatonin,
natural
hormone,
on
migration
cells
two
cell
lines,
MCF‐7
MDA‐MB‐231.
MDA‐MB‐231
were
cultured
their
respective
media.
The
effective
dose
melatonin
each
line
was
determined
using
MTT
assay.
IC50
assessed
wound‐healing
expression
invasion‐related
genes
(MMP2
MMP9),
as
well
receptors
MT1
MT2,
analyzed
Real‐Time
RT‐PCR.
assay
results
indicated
that
48
h
treatment
at
doses
2.5
3.5
M
significantly
reduced
cells.
In
addition,
decreased
markers
both
lines.
Melatonin
also
increased
MT2
MMP2
MMP9
by
(
p
<
0.05).
Our
indicate
naturally
occurring
compound,
possesses
potential
to
inhibit
movement
spread
breast
elevating
levels
receptors,
resulting
reduction
matrix
metalloproteinases
2
9
expression.
Journal of Biochemical and Molecular Toxicology,
Год журнала:
2025,
Номер
39(3)
Опубликована: Фев. 26, 2025
ABSTRACT
Melatonin
exhibits
various
biological
functions,
including
regulation
of
circadian
and
endocrine
rhythms,
anti‐inflammatory,
antioxidant
effects.
Aging
damaged
mitochondria
are
major
sources
oxidative
stress
(OS),
mitochondrial
quality
control
(MQC)
is
crucial
for
maintaining
normal
function.
Myocardial
ischemia‐reperfusion
injury
a
complication
that
can
arise
during
reperfusion
therapy
coronary
heart
disease.
However,
effective
intervention
strategies
currently
lacking.
Mitochondrial
dysfunction
OS
considered
central
mechanisms
myocardial
injury,
with
mitochondrial‐targeted
interventions
being
potential
treatment
direction.
Recent
studies
have
shown
melatonin
improves
structure
function
through
multiple
pathways.
This
review
discusses
the
by
which
ameliorates
focusing
on
MQC,
explores
its
applications
in
prevention
injury.
Biochemical Journal,
Год журнала:
2025,
Номер
482(06), С. 309 - 324
Опубликована: Март 1, 2025
Quinone
reductase
2
(NQO2)
is
a
FAD-linked
enzyme
that
cannot
use
the
common
reducing
cofactors,
NADH
and
NADPH,
for
efficient
catalysis.
This
unusual
an
oxidoreductase,
particularly
since
it
member
of
large
family
enzymes
all
NAD(P)H
efficiently
to
catalyse
two-electron
reduction
in
quinones
other
electrophiles.
The
inability
NQO2
raises
questions
about
its
cellular
function:
remains
unclear
whether
main
role
catalytic
or
pseudo-enzyme
with
roles
such
as
cell
signalling.
Intriguingly,
has
been
identified
off-target
interactor
over
30
kinase
inhibitors
drugs
natural
products.
interaction
between
kinase-targeted
intriguing
because
suggests
may
be
contributing
effects
these
drugs.
In
this
review,
we
will
discuss
enzymatic
properties
NQO2,
structure
complexes
various
small
molecules,
potential
roles,
some
enigmatic
findings
make
molecule
so
interesting
worthy
further
investigation.
