Multiplex genome editing for climate-resilient woody plants

Frontiers in Forests and Global Change, Год журнала: 2025, Номер 8

Опубликована: Фев. 5, 2025

Climate change is severely impacting global forest ecosystems, stressing woody plants due to rising temperatures, shifting precipitation patterns, and extreme weather events. These pressures threaten biodiversity disrupt the essential roles forests play in carbon sequestration, timber production, ecosystem stability. Traditional management strategies, such as selective breeding, cannot keep up with rapid pace of climate change, given long juvenile phase trees. Multiplex genome editing, particularly through CRISPR technologies, offers a promising solution accelerate development climate-resilient traits plants. By simultaneously targeting multiple genes, multiplex enables efficient modification polygenic that govern stress tolerance, disease resistance, other crucial resilience factors. This mini-review examines potential technologies management, agroecological practices, showing how they can improve tree support sustainable forestry response growing challenges change.

Язык: Английский

The Potential of Transgenic Hybrid Aspen Plants with a Recombinant Lac Gene from the Fungus Trametes hirsuta to Degrade Trichlorophenol

Genes, Год журнала: 2025, Номер 16(3), С. 298 - 298

Опубликована: Фев. 28, 2025

Objective: Laccases are known to be able degrade phenolic compounds simpler components. The main objective of our study was analyze this property in transgenic aspen plants carrying the laccase gene Lac from Trametes hirsuta which can potentially used soil phytoremediation. Methods: We created hirsute using agrobacterial transformation stem explants with pBI–Lac vector containing white rot fungus T. 072 (NCBI GenBank accession number KP027478). Transgenic were micropropagated and cultivated vitro lines. degradation 2,4,6-trichlorophenol (2,4,6-TCP) by plant roots analyzed mass-spectrometry electron ionization a gas chromatograph. Results: Although have their own laccases, those fungal origin more effective. All that expressed recombinant degraded 2,4,6-TCP effectively than non-transformed control (the efficiency ranged 92 98% versus 82% control). Line 47Lac8 demonstrated 16% higher control. There also an inverse relationship between viability line its level expression gene. Thus, 47Lac4 not viable under native conditions, probably due lignin synthesis disruptions during initiation secondary tissues. This is confirmed changes genes biosynthesis. rest lines did differ significantly wood growth biochemistry. shown preserve ability express ex vitro. Conclusions: Three (47Lac5, 47Lac8, 47Lac23) recommended for use

Язык: Английский

Forest Tree Breeding Under the Global Environmental Change: Challenges and Opportunities

Trees Forests and People, Год журнала: 2025, Номер unknown, С. 100867 - 100867

Опубликована: Апрель 1, 2025

Язык: Английский